scholarly journals Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function

2014 ◽  
Vol 1 (2) ◽  
pp. 123 ◽  
Author(s):  
Jennifer Borcherding ◽  
Jonas Baltrusaitis ◽  
Haihan Chen ◽  
Larissa Stebounova ◽  
Chia-Ming Wu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Iron Oxide ◽  
Antimicrobial Peptide ◽  
Iron Oxide Nanoparticles ◽  
Biofilm Formation ◽  
Oxide Nanoparticles

scholarly journals The Effect of Gold and Iron-Oxide Nanoparticles on Biofilm-Forming Pathogens

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Madhu Bala Sathyanarayanan ◽  
Reneta Balachandranath ◽  
Yuvasri Genji Srinivasulu ◽  
Sathish Kumar Kannaiyan ◽  
Guruprakash Subbiahdoss
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Immune Cells ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Medical Applications ◽  
Oxide Nanoparticles ◽  
Biofilm Growth ◽  
Microbial Biofilms ◽  
Exopolymeric Substances

Microbial biofilms on biomaterial implants or devices are hard to eliminate by antibiotics due to their protection by exopolymeric substances that embed the organisms in a matrix, impenetrable for most antibiotics and immune-cells. Application of metals in their nanoparticulated form is currently considered to resolve bacterial infections. Gold and iron-oxide nanoparticles are widely used in different medical applications, but their utilisation to eradicate biofilms on biomaterials implants is novel. Here, we studied the effect of gold and iron oxide nanoparticles on Staphylococcus aureus and Pseudomonas aeruginosa biofilms. We report that biofilm growth was reduced at higher concentrations of gold and iron-oxide nanoparticles compared to absence of nanoparticles. Thus nanoparticles with appropriate concentration could show significant reduction in biofilm formation.

scholarly journals Reduced Staphylococcus aureus biofilm formation in the presence of chitosan-coated iron oxide nanoparticles

2016 ◽  
Vol Volume 11 ◽  
pp. 6499-6506 ◽  
Author(s):  
Sifeng Shi ◽  
Jingfu Jia ◽  
XiaoKui Guo ◽  
Yaping Zhao ◽  
Desheng Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Biofilm Formation ◽  
Oxide Nanoparticles

scholarly journals Antibacterial Efficacy of Iron-Oxide Nanoparticles against Biofilms on Different Biomaterial Surfaces

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Monica Thukkaram ◽  
Soundarya Sitaram ◽  
Sathish kumar Kannaiyan ◽  
Guruprakash Subbiahdoss
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Polymer Brush ◽  
Oxide Nanoparticles ◽  
Implant Surface ◽  
Biofilm Growth ◽  
Biomaterial Surfaces ◽  
Coated Surfaces

Biofilm growth on the implant surface is the number one cause of the failure of the implants. Biofilms on implant surfaces are hard to eliminate by antibiotics due to the protection offered by the exopolymeric substances that embed the organisms in a matrix, impenetrable for most antibiotics and immune cells. Application of metals in nanoscale is considered to resolve biofilm formation. Here we studied the effect of iron-oxide nanoparticles over biofilm formation on different biomaterial surfaces and pluronic coated surfaces. Bacterial adhesion for 30 min showed significant reduction in bacterial adhesion on pluronic coated surfaces compared to other surfaces. Subsequently, bacteria were allowed to grow for 24 h in the presence of different concentrations of iron-oxide nanoparticles. A significant reduction in biofilm growth was observed in the presence of the highest concentration of iron-oxide nanoparticles on pluronic coated surfaces compared to other surfaces. Therefore, combination of polymer brush coating and iron-oxide nanoparticles could show a significant reduction in biofilm formation.

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

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