Selection of a DNA aptamer for cadmium detection based on cationic polymer mediated aggregation of gold nanoparticles

The Analyst ◽  
2014 ◽  
Vol 139 (6) ◽  
pp. 1550-1561 ◽  
Author(s):  
Yuangen Wu ◽  
Shenshan Zhan ◽  
Lumei Wang ◽  
Pei Zhou
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Cationic Polymer ◽  
Dna Aptamer ◽  
Recognition Element ◽  
Cadmium Detection ◽  
Selection Of

The DNA aptamer toward cadmium was selected by a novel SELEX strategy and further considered as a recognition element for the colorimetric detection of Cd(ii).

Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles

2017 ◽  
Vol 41 (19) ◽  
pp. 10592-10597 ◽  
Author(s):  
Fei Qu ◽  
Yanqun Liu ◽  
Haili Lao ◽  
Yaping Wang ◽  
Jinmao You
Keyword(s):  
Gold Nanoparticles ◽  
Au Nanoparticles ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Cationic Polymer ◽  
Polymer Nanoparticles

The negatively charged heparin hinders the aggregation of Au nanoparticles induced by the cationic polymer nanodots.

Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles

2016 ◽  
Vol 499 ◽  
pp. 51-56 ◽  
Author(s):  
Dongwei Zhang ◽  
Jiayi Yang ◽  
Jing Ye ◽  
Lurong Xu ◽  
Hanchu Xu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bisphenol A ◽  
Colorimetric Detection ◽  
Cationic Polymer

Colorimetric aptasensor for detecting Salmonella spp., Listeria monocytogenes, and Escherichia coli in meat samples

2020 ◽  
Vol 26 (5) ◽  
pp. 430-443 ◽  
Author(s):  
Sudarat Ledlod ◽  
Supatra Areekit ◽  
Somchai Santiwatanakul ◽  
Kosum Chansiri
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Listeria Monocytogenes ◽  
Colorimetric Detection ◽  
Simultaneous Detection ◽  
Dna Aptamer ◽  
Local Market ◽  
Salmonella Spp ◽  
Meat Samples ◽  
Colorimetric Aptasensor

In this study, we successfully developed a simple and rapid method for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli using gold nanoparticles and the aptamer aptasensor. We screened 25 specific DNA aptamer candidates against these pathogens using whole-cell Systematic Evolution of Ligands by EXponential enrichment. Among them, Ap6 was selected due to its low energy minimization values of −12.25 and −27.67 kcal/mol derived from MFold and RNAFold analysis, respectively. The assay presented in this study allowed the visual colorimetric detection of labeled colloidal gold nanoparticles as well as determination of UV absorbance at 625 and 525 nm under optimized conditions. The detection limit of this aptasensor was as less as 105 CFU/ml. A random investigation of 50 meat samples, including ham and chicken sausages, collected from the local market revealed 96% accuracy, 96% specificity, and 100% sensitivity of the assay. The colorimetric aptasensor can accomplish one-step detection without pre-culture, DNA extraction, and amplification. Hence, it is an easy, rapid, specific, and qualitative assay that can be used as a point-of-care testing to directly detect multiplex foodborne pathogens.

scholarly journals Target-Induced Aggregation of Gold Nanoparticles for Colorimetric Detection of Bisphenol A

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Sung Hyun Hwang ◽  
Sehan Jeong ◽  
Hyung Joo Choi ◽  
Hyunmin Eun ◽  
Min Geun Jo ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Bisphenol A ◽  
Tap Water ◽  
Colorimetric Detection ◽  
Daily Intake ◽  
Consumer Products ◽  
Dna Aptamer ◽  
Site Quality ◽  
Local Market ◽  
Induced Aggregation

Bisphenol A (BPA) is used in a wide variety of consumer products owing to its beneficial properties of optical clarity, shatter resistance, and heat resistance. However, leached BPA has been shown to disturb the endocrine system and could cause cancer even at low concentrations, which has led to public concern. To reduce the toxic effects caused by BPA, it is important to monitor the BPA levels and its presence in products in a simple, rapid, and on-site manner. Here, we propose a new colorimetric strategy for the simple and rapid detection of BPA employing a DNA aptamer, a cationic surfactant, and gold nanoparticles (AuNPs). Using the developed system, the presence of BPA can be successfully determined based simply on a visually detectable color change from red to blue, triggered by aggregate formation of the AuNPs, which can be monitored even with the naked eye. Under the optimized conditions, this system could detect BPA with excellent selectivity and sensitivity, and its high performance was validated in the receipt obtained from local market and BPA-spiked tap water samples, ensuring its practical applicability. Moreover, the limit of the detection of the system was determined to be 97 nM, which is below the current tolerable daily intake level, demonstrating its suitability for toxicity assessment and on-site quality control in a more economical manner when compared with conventional methods.

A Label-Free Colorimetric Biosensor for 17β-Estradiol Detection Using Nanoparticles Assembled by Aptamer and Cationic Polymer

2016 ◽  
Vol 69 (1) ◽  
pp. 12 ◽  
Author(s):  
Dongwei Zhang ◽  
Weilin Zhang ◽  
Jiayun Ye ◽  
Shenshan Zhan ◽  
Bing Xia ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Colorimetric Detection ◽  
Cationic Polymer ◽  
Label Free ◽  
Human Beings ◽  
Remarkable Change ◽  
Environment Effects ◽  
Diallyldimethylammonium Chloride ◽  
17Β Estradiol

Concern is mounting regarding the human health and environment effects of 17β-estradiol (E2), a natural oestrogen excreted by human beings and animals. In this paper, a sensitive and selective biosensor for the detection of E2 using gold nanoparticles (AuNPs), label-free E2-specific aptamer, and poly(diallyldimethylammonium chloride) (PDDA) was developed. In the absence of E2, PDDA can electrostatically interact with E2-specific aptamer, and the charge responsible for inducing AuNPs aggregation was destroyed. However, the introduction of E2 can specifically interact with the aptamer to form E2–aptamer complex so that PDDA can aggregate AuNPs and cause a remarkable change in colour from wine red to blue, which enables colorimetric detection of E2 with selectivity and a detection limit of 1.57 nM.

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

Naked-eye colorimetric detection of HCV RNA mediated by a 5′ UTR-targeted antisense oligonucleotide and plasmonic gold nanoparticles

The Analyst ◽  
2021 ◽  
Author(s):  
Almas Shamaila Mohammed ◽  
Aniket Balapure ◽  
Mahammad Nanne Khaja ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta
Keyword(s):  
Gold Nanoparticles ◽  
Antisense Oligonucleotide ◽  
Early Stage ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Clinical Samples ◽  
Hcv Rna ◽  
Naked Eye

An Au NP based facile strategy for the rapid, early-stage, and sensitive detection of HCV RNA in clinical samples which avoids thiol tagging to the antisense oligonucleotide and expensive infrastructure is presented.

scholarly journals Colorimetric detection of Cr3+ based on gold nanoparticles functionalized with 4-mercaptobenzoic acid

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhikun Zhang ◽  
Xiaojie Ye ◽  
Qingqing Liu ◽  
Yumin Liu ◽  
Runjing Liu
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection
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