scholarly journals Simple and Convenient G-Quadruplex-Based Turn-On Fluorescence Assay for 3′ → 5′ Exonuclease Activity

2011 ◽  
Vol 83 (2) ◽  
pp. 463-466 ◽  
Author(s):  
Chung-Hang Leung ◽  
Daniel Shiu-Hin Chan ◽  
Bradley Yat-Wah Man ◽  
Chuan-Jen Wang ◽  
Wing Lam ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Exonuclease Activity ◽  
Turn On ◽  
G Quadruplex

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

Comparison of turn-on and ratiometric fluorescent G-quadruplex aptasensor approaches for the detection of ATP

2019 ◽  
Vol 411 (7) ◽  
pp. 1319-1330 ◽  
Author(s):  
Sathya Srinivasan ◽  
Velu Ranganathan ◽  
Maria C. DeRosa ◽  
Bhaskar Mohan Murari
Keyword(s):  
Turn On ◽  
G Quadruplex

Imaging nucleus viscosity and G-quadruplex DNA in living cells using a nucleus-targeting two-photon fluorescent probe

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5799-5804 ◽  
Author(s):  
Wan Sun ◽  
Jing-Xue Cui ◽  
Le-Le Ma ◽  
Zhong-Lin Lu ◽  
Bing Gong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Dna Detection ◽  
Living Cells ◽  
Cell Nuclei ◽  
Quadruplex Dna ◽  
Two Photon ◽  
Turn On ◽  
G Quadruplex

TP-2Bz, as a nucleus targeting “D–π–A” molecule, was developed as a two-photon turn-on fluorescent probe for the viscosity and GQ DNA detection in cell nuclei.

scholarly journals Correction to: Comparison of turn-on and ratiometric fluorescent G-quadruplex aptasensor approaches for the detection of ATP

2019 ◽  
Vol 411 (7) ◽  
pp. 1491-1491
Author(s):  
Sathya Srinivasan ◽  
Velu Ranganathan ◽  
Maria C. DeRosa ◽  
Bhaskar Mohan Murari
Keyword(s):  
Turn On ◽  
G Quadruplex

scholarly journals Design of a Fluorescence Turn-on and Label-free Aptasensor Using the Intrinsic Quenching Power of G-Quadruplex to AMT

2020 ◽  
Vol 36 (8) ◽  
pp. 965-970
Author(s):  
Dandan WANG ◽  
Fenghua GENG ◽  
Yongxiang WANG ◽  
Yu MA ◽  
Guixin LI ◽  
...  
Keyword(s):  
Label Free ◽  
Turn On ◽  
G Quadruplex ◽  
Fluorescence Turn On

A “turn-on” and label-free fluorescent assay for the rapid detection of exonuclease III activity based on Tb3+-induced G-quadruplex conjugates

2014 ◽  
Vol 406 (18) ◽  
pp. 4535-4540 ◽  
Author(s):  
WeiJuan Yang ◽  
YaJuan Ruan ◽  
WeiHua Wu ◽  
PingPing Chen ◽  
LiangJun Xu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Label Free ◽  
Fluorescent Assay ◽  
Exonuclease Iii ◽  
Turn On ◽  
G Quadruplex
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