Graphene oxide for rapid microRNA detection

Nanoscale ◽  
2012 ◽  
Vol 4 (19) ◽  
pp. 5840 ◽  
Author(s):  
Zhuoxuan Lu ◽  
Liming Zhang ◽  
Yan Deng ◽  
Song Li ◽  
Nongyue He
Keyword(s):  
Graphene Oxide ◽  
Microrna Detection

scholarly journals A flexible smart membrane consisting of GO composite fibres and upconversion MSNs for microRNA detection

2019 ◽  
Vol 55 (62) ◽  
pp. 9104-9107 ◽  
Author(s):  
Tongxu Gu ◽  
Zhaohui Ren ◽  
Xiang Li ◽  
Jie Huang ◽  
Gaorong Han
Keyword(s):  
Graphene Oxide ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Dna Probes ◽  
Single Stranded Dna ◽  
Microrna Detection ◽  
Flexible Polyurethane

We have developed a photoluminescent membrane for microRNA detection, consisting of mesoporous silica nanoparticles (CaF2:Yb/Ho@MSNs) attached, via single stranded DNA probes, to flexible polyurethane fibres coated with graphene oxide (GO).

scholarly journals Molecular mechanism of helicase on graphene-based hybridization reaction platform for microRNA detection

RSC Advances ◽  
2017 ◽  
Vol 7 (58) ◽  
pp. 36444-36449 ◽  
Author(s):  
Xiaojun Fan ◽  
Yi Qi ◽  
Zhaoyi Shi ◽  
Yongkang Lv ◽  
Yujing Guo
Keyword(s):  
Escherichia Coli ◽  
Graphene Oxide ◽  
Molecular Mechanism ◽  
Recq Helicase ◽  
Microrna Detection ◽  
First Time ◽  
Hybridization Reaction

The Escherichia coli RecQ helicase was introduced for the first time to demonstrate its molecular mechanism on hybridization reaction for the detection of microRNA, using graphene oxide (GO) as nano-quencher.

Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction

2012 ◽  
Vol 84 (10) ◽  
pp. 4587-4593 ◽  
Author(s):  
Haifeng Dong ◽  
Jing Zhang ◽  
Huangxian Ju ◽  
Huiting Lu ◽  
Shiyan Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive

A simple and rapid dual-cycle amplification strategy for microRNA based on graphene oxide and exonuclease III-assisted fluorescence recovery

2018 ◽  
Vol 10 (30) ◽  
pp. 3777-3782 ◽  
Author(s):  
Yafang Tang ◽  
Mingxiu Liu ◽  
Lingcao Xu ◽  
Jianniao Tian ◽  
Xiulin Yang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Detection Method ◽  
Fluorescence Recovery ◽  
Exonuclease Iii ◽  
Microrna Detection ◽  
Dual Cycle ◽  
Exo Iii ◽  
Amplification Strategy

A simple microRNA detection method by combining Graphene Oxide (GO) fluorescence quenching with exonuclease III (Exo-III) aided cycling amplification was developed.

scholarly journals A new graphene oxide enhanced fluorescence anisotropy strategy for microRNA detection

2017 ◽  
Vol 48 (1) ◽  
pp. 85-92
Author(s):  
Jing Tao ◽  
Xue Xiao ◽  
Chunhong Li ◽  
Chen Men ◽  
Shujun Zhen
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Anisotropy ◽  
Enhanced Fluorescence ◽  
Microrna Detection

scholarly journals Nonenzymatic DNA-Based Fluorescence Biosensor Combining Carbon Dots and Graphene Oxide with Target-Induced DNA Strand Displacement for microRNA Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2608
Author(s):  
Yuanyuan Gao ◽  
Hong Yu ◽  
Jingjing Tian ◽  
Botao Xiao
Keyword(s):  
Graphene Oxide ◽  
Carbon Dots ◽  
Signal Amplification ◽  
Strand Displacement ◽  
Fluorescence Sensing ◽  
Microrna Detection ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand ◽  
Fluorescence Biosensor

Based on a fluorescence “on-off-on” strategy, we fabricated a simple and highly sensitive DNA-based fluorescence biosensor for the detection of micro (mi)RNA from carbon dots (CDs) and graphene oxide (GO) without complicated and time-consuming operations. CDs were successfully synthesized and conjugated to the end of a single-stranded fuel DNA that was adsorbed onto the surface of GO through π-π stacking, resulting in fluorescence quenching. In the presence of the target miRNA let-7a, the fuel DNA was desorbed from the GO surface, and fluorescence was restored through two successive toehold-mediated strand displacement reactions on double-stranded DNA-modified gold nanoparticles. The target miRNA let-7a was recycled, leading to signal amplification. The concentration of let-7a was proportional to the degree of fluorescence recovery. Under optimal conditions, there was a good linear relationship between the relative fluorescence intensity and let-7a concentration in the range of 0.01–1 nM, with a detection limit of 7.8 pM. With its advantages of signal amplification and high biocompatibility, this fluorescence sensing strategy can be applied to the detection of a variety of target miRNAs and can guide the design of novel biosensors with improved properties.

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