Highly Sensitive Multiple microRNA Detection Based on Fluorescence Quenching of Graphene Oxide and Isothermal Strand-Displacement Polymerase Reaction

2012 ◽  
Vol 84 (10) ◽  
pp. 4587-4593 ◽  
Author(s):  
Haifeng Dong ◽  
Jing Zhang ◽  
Huangxian Ju ◽  
Huiting Lu ◽  
Shiyan Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive

HIGHLY SENSITIVE METHODS FOR MICRORNA DETECTION USING ISOTHERMAL METHODS FOR NUCLEIC ACIDS AMPLIFICATION

2021 ◽  
Vol 1 (19) ◽  
pp. 244-246
Author(s):  
O.L. Bodulev ◽  
A.M. Soloviev
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly

This work will present the results of the development of two new highly sensitive heterogeneous methods for the determination of miRNA-141 and miRNA-39 using the isothermal circular strand-displacement amplification and the catalytic hairpin assembly methods for analyte amplification.

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

2014 ◽  
Vol 50 (73) ◽  
pp. 10691-10694 ◽  
Author(s):  
Lijuan Ji ◽  
Zhewei Cai ◽  
Yingdan Qian ◽  
Ping Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Restriction Endonuclease ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Site Specific ◽  
Inhibitor Screening ◽  
Selective Approach ◽  
Highly Sensitive

A sensitive and selective approach for the DNA methyltransferase activity assay and MTase inhibitor screening is reported.

An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

2018 ◽  
Vol 54 (77) ◽  
pp. 10897-10900 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

A simple and rapid dual-cycle amplification strategy for microRNA based on graphene oxide and exonuclease III-assisted fluorescence recovery

2018 ◽  
Vol 10 (30) ◽  
pp. 3777-3782 ◽  
Author(s):  
Yafang Tang ◽  
Mingxiu Liu ◽  
Lingcao Xu ◽  
Jianniao Tian ◽  
Xiulin Yang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Detection Method ◽  
Fluorescence Recovery ◽  
Exonuclease Iii ◽  
Microrna Detection ◽  
Dual Cycle ◽  
Exo Iii ◽  
Amplification Strategy

A simple microRNA detection method by combining Graphene Oxide (GO) fluorescence quenching with exonuclease III (Exo-III) aided cycling amplification was developed.

A novel graphene oxide based fluorescent nanosensing strategy with hybridization chain reaction signal amplification for highly sensitive biothiol detection

2014 ◽  
Vol 50 (80) ◽  
pp. 11879-11882 ◽  
Author(s):  
Jia Ge ◽  
Zhi-Mei Huang ◽  
Qiang Xi ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Signal Amplification ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive

A novel fluorescent nanosensor was developed for detecting biothiols via coupling graphene based fluorescence quenching with T–Hg(ii)–T controlled hybridization chain reaction.

scholarly journals Nonenzymatic DNA-Based Fluorescence Biosensor Combining Carbon Dots and Graphene Oxide with Target-Induced DNA Strand Displacement for microRNA Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2608
Author(s):  
Yuanyuan Gao ◽  
Hong Yu ◽  
Jingjing Tian ◽  
Botao Xiao
Keyword(s):  
Graphene Oxide ◽  
Carbon Dots ◽  
Signal Amplification ◽  
Strand Displacement ◽  
Fluorescence Sensing ◽  
Microrna Detection ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand ◽  
Fluorescence Biosensor

Based on a fluorescence “on-off-on” strategy, we fabricated a simple and highly sensitive DNA-based fluorescence biosensor for the detection of micro (mi)RNA from carbon dots (CDs) and graphene oxide (GO) without complicated and time-consuming operations. CDs were successfully synthesized and conjugated to the end of a single-stranded fuel DNA that was adsorbed onto the surface of GO through π-π stacking, resulting in fluorescence quenching. In the presence of the target miRNA let-7a, the fuel DNA was desorbed from the GO surface, and fluorescence was restored through two successive toehold-mediated strand displacement reactions on double-stranded DNA-modified gold nanoparticles. The target miRNA let-7a was recycled, leading to signal amplification. The concentration of let-7a was proportional to the degree of fluorescence recovery. Under optimal conditions, there was a good linear relationship between the relative fluorescence intensity and let-7a concentration in the range of 0.01–1 nM, with a detection limit of 7.8 pM. With its advantages of signal amplification and high biocompatibility, this fluorescence sensing strategy can be applied to the detection of a variety of target miRNAs and can guide the design of novel biosensors with improved properties.

Characterization of Graphene Oxide Suspension for Fluorescence Quenching in DNA-Diagnostics

2016 ◽  
Vol 26 (1) ◽  
pp. 1-7 ◽  
Author(s):  
A.N. Kapitonov ◽  
G.N. Alexandrov ◽  
F.D. Vasileva ◽  
S.A. Smagulova ◽  
V.B. Timofeev ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Dna Diagnostics
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