Simultaneous detection of duplex DNA oligonucleotides using a SERS-based micro-network gradient chip

Lab on a Chip ◽  
2012 ◽  
Vol 12 (24) ◽  
pp. 5160 ◽  
Author(s):  
Namhyun Choi ◽  
Kangsun Lee ◽  
Dong Woo Lim ◽  
Eun Kyu Lee ◽  
Soo-Ik Chang ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Duplex Dna ◽  
Dna Oligonucleotides

Mechanism for Radical Cation Transport in Duplex DNA Oligonucleotides

2004 ◽  
Vol 126 (9) ◽  
pp. 2877-2884 ◽  
Author(s):  
Chu-Sheng Liu ◽  
Rigoberto Hernandez ◽  
Gary B. Schuster
Keyword(s):  
Radical Cation ◽  
Cation Transport ◽  
Duplex Dna ◽  
Dna Oligonucleotides

Modeling Duplex DNA Oligonucleotides with Modified Pyrimidine Bases

1997 ◽  
pp. 312-328
Author(s):  
John Miller ◽  
Michael Cooney ◽  
Karol Miaskiewicz ◽  
Roman Osman
Keyword(s):  
Duplex Dna ◽  
Dna Oligonucleotides ◽  
Pyrimidine Bases

Species Specific Immunoassays to Measure Blood Platelet and Coagulation Activation in the Rat

1996 ◽  
Vol 76 (06) ◽  
pp. 1090-1095 ◽  
Author(s):  
C Ravanat ◽  
M Freund ◽  
S Schuhler ◽  
P Grunert ◽  
L Meyer ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Plasma Concentrations ◽  
Simultaneous Detection ◽  
Blood Platelet ◽  
Coagulation Activation ◽  
Platelet Factor ◽  
Prethrombotic State ◽  
Low Levels ◽  
Species Specific ◽  
Higher Sensitivity

SummaryThe purpose of this study was to develop specific and sensitive immunoassays to detect early indices of hypercoagulability in the rat. Rat platelet factor 4 (rPF4) and rat fibrinopeptide A (rFPA) assays, tools for the detection of activation of platelets and coagulation respectively, were designed using antibodies raised against purified rPF4 and against synthetic rFPA. The relevance of these new assays and of the commercially available ELISA kit for thrombin-antithrombin III (TAT) complexes was demonstrated in a rat model of a prethrombotic state induced by intravenous infusion of varying doses of thromboplastin (90 to 2400 μl/kg/h). In this model, the immunoassays allowed simultaneous detection of low levels of rFPA and rPF4 which were correlated with fibrinogen and platelet consumption and TAT generation and further proved to be of higher sensitivity than the classical methods of platelet count or measurement of fibrinogen levels. Plasma concentrations of rFPA, rPF4 and TAT were dependent on infusion time and thromboplastin dose, while hirudin (1 mg/kg) prevented their appearance. Thus the new specific immunoassays for rPF4 and rFPA and the commercial human TAT assay represent useful tools for pathophysiological studies or the screening of antithrombotic drugs in rats.

Effect of Netropsin on One-electron Oxidation of Duplex DNA¶†

2004 ◽  
Vol 80 (3) ◽  
pp. 456 ◽  
Author(s):  
Lezah W. Roberts ◽  
Gary B. Schuster
Keyword(s):  
Duplex Dna
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