Analysis of the Raman spectra of Ca2+-dipicolinic acid alone and in the bacterial spore core in both aqueous and dehydrated environments

The Analyst ◽  
2012 ◽  
Vol 137 (16) ◽  
pp. 3683 ◽  
Author(s):  
Lingbo Kong ◽  
Peter Setlow ◽  
Yong-qing Li
Keyword(s):  
Raman Spectra ◽  
Dipicolinic Acid ◽  
Bacterial Spore

In Situ Surface-Etched Bacterial Spore Detection Using Dipicolinic Acid–Europium–Silica Nanoparticle Bioreporters

2011 ◽  
Vol 65 (8) ◽  
pp. 866-875 ◽  
Author(s):  
Clint B. Smith ◽  
John E. Anderson ◽  
Jarrod D. Edwards ◽  
Kinson C. Kam
Keyword(s):  
Dipicolinic Acid ◽  
Silica Nanoparticle ◽  
Bacterial Spore ◽  
Spore Detection

UV Resonance Raman Spectra of Bacillus Spores

1992 ◽  
Vol 46 (2) ◽  
pp. 357-364 ◽  
Author(s):  
E. Ghiamati ◽  
R. Manoharan ◽  
W. H. Nelson ◽  
J. F. Sperry
Keyword(s):  
Aqueous Solutions ◽  
Bacillus Cereus ◽  
Raman Spectra ◽  
Dipicolinic Acid ◽  
Resonance Raman ◽  
Vegetative Cells ◽  
Resonance Enhancement ◽  
Uv Resonance Raman ◽  
Bacillus Spores ◽  
Resonance Raman Spectra

UV resonance Raman spectra of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis endospores have been excited at 222.7,230.7,242.5, and 251.1 nm, and spectra have been compared with those of vegetative cells. The resonance Raman spectra of aqueous solutions of dipicolinic acid and calcium dipicolinate have been measured at the same wavelengths. Spectra of endospores and their corresponding germinated spores show only modest differences when excited at 222, 231, and 251 nm. However, very substantial differences appear when excitation occurs at 242 nm. Difference spectra obtained at 242 nm by subtracting spectra of germinated spores of Bacillus cereus from spectra of their corresponding endospores are attributed almost entirely to dipicolinate. Vegetative cells and endospores show large spectral dissimilarities at all exciting wavelengths. These spectral differences, which vary strongly with exciting wavelength, appear to be the result of large differences in the amounts and composition of proteins and nucleic acids, especially ribosomal RNA. The very substantial resonance enhancement of Raman spectra has been obtained from aqueous solutions of pure dipicolinic acid and of sodium and calcium dipicolinate salts, as well as spores at the various exciting wavelengths. The strong enhancement of dipicolinate spectra in spores, however, was noted only with 242-nm excitation. Consequently, only with 242-nm light was it possible to selectively and sensitively excite and study calcium dipicolinate in spores. Resonance enhancement of the dipicolinate spectra with 242-nm excitation appears due primarily to resonance interactions with n-π* electronic transitions associated with the pyridine ring and/or the carboxylate group.

scholarly journals Studies on a Biosynthethic Site in Dipicolinic Acid Formation by Bacterial Spore Formers

1967 ◽  
Vol 22 (9) ◽  
pp. 505-509
Author(s):  
Chikataro KAWASAKI ◽  
Masaomi KONDO ◽  
Jun SAKURAI
Keyword(s):  
Dipicolinic Acid ◽  
Bacterial Spore ◽  
Acid Formation

scholarly journals Mode of Action of Disinfection Chemicals on the Bacterial Spore Structure and Their Raman Spectra

2021 ◽  
Vol 93 (6) ◽  
pp. 3146-3153
Author(s):  
Dmitry Malyshev ◽  
Tobias Dahlberg ◽  
Krister Wiklund ◽  
Per Ola Andersson ◽  
Sara Henriksson ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Mode Of Action ◽  
Bacterial Spore

scholarly journals Spontaneous Raman spectra of dipicolinic acid in microcrystalline form

2003 ◽  
Vol 50 (15-17) ◽  
pp. 2369-2374
Author(s):  
Sergei N. Jerebtsov ◽  
Tomas Opatrny ◽  
Hans A. Schuessler ◽  
Marlan O. Scully ◽  
Alexandre A. Kolomenskii
Keyword(s):  
Raman Spectra ◽  
Dipicolinic Acid

scholarly journals Electro-Mechanical Stresses on Bacillus Atrophaeus Bacterial Spore Germination Induced by Pulsed Electric Fields

2020 ◽  
Author(s):  
Xing Qiu ◽  
Wen Jie WU
Keyword(s):  
Electric Fields ◽  
Spore Germination ◽  
Dipicolinic Acid ◽  
Deep Understanding ◽  
Pulsed Electric Fields ◽  
Mechanical Stresses ◽  
Food Preservation ◽  
Bacterial Spore ◽  
Bacillus Atrophaeus ◽  
Thickness Change

Abstract In this work, the electro-mechanical stresses induced by pulsed electric fields (PEF) on bacterial spore germination was modeled and empirically tested using Bacillus atrophaeus spores. Specifically, a new model, termed as Qiu-Wu’s electro-mechanical (QW’s EM) spore model, was derived to analyze the effect of electro-mechanical stresses on spores subjected under electric fields. A non-linear inverse relationship was found between electric fields and thickness change of spore coat. SEM, pH, hydrophobicity and electrochemical measurements were implemented for verification of the model. PEF-treated spores germinated with a faster rate and a higher degree of homogeneity. The longer the treatment time, the better the homogeneity. The speed of dipicolinic acid (DPA) release was around 20% faster in PEF-treated samples, while the peak intensity of terbium-DPA from PEF-treated samples was up to 80% lower. Theoretical analysis and empirical results were consistent to show that PEF introduces electro-mechanical stresses to expedite spore germination. The significance and impact of this study is obvious: bacterial spore is implicated in food spoilage and foodborne diseases primarily via the process of germination, and PEF technology has been introduced to inactivate microorganisms in food. Understanding the mechanism of germination under PEF can provide deep understanding for inactivation of foodborne pathogens and better food preservation methods.

scholarly journals Studies on a Biosynthetic Site in Dipicolinic Acid Formation by Bacterial Spore Formers

1967 ◽  
Vol 22 (8) ◽  
pp. 463-467 ◽  
Author(s):  
Chikataro KAWASAKI ◽  
Masaomi KONDO ◽  
Tsutomu NISHIHARA
Keyword(s):  
Dipicolinic Acid ◽  
Bacterial Spore ◽  
Acid Formation

scholarly journals Analysis for bacterial spore-specific dipicolinic acid by high-pressure liquid chromatography.

1988 ◽  
Vol 43 (5) ◽  
pp. 927-930 ◽  
Author(s):  
Kazuhito WATABE ◽  
Kyoko MAEKAWA ◽  
Atsumi YAMADA ◽  
Tomihiko KOSHIKAWA ◽  
Setsuko OGAWA ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Dipicolinic Acid ◽  
Bacterial Spore
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