In Situ Surface-Etched Bacterial Spore Detection Using Dipicolinic Acid–Europium–Silica Nanoparticle Bioreporters

2011 ◽  
Vol 65 (8) ◽  
pp. 866-875 ◽  
Author(s):  
Clint B. Smith ◽  
John E. Anderson ◽  
Jarrod D. Edwards ◽  
Kinson C. Kam
Keyword(s):  
Dipicolinic Acid ◽  
Silica Nanoparticle ◽  
Bacterial Spore ◽  
Spore Detection

Bacterial Spore Detection and Determination by Use of Terbium Dipicolinate Photoluminescence

1997 ◽  
Vol 69 (6) ◽  
pp. 1082-1085 ◽  
Author(s):  
David L. Rosen ◽  
Charles Sharpless ◽  
Linda B. McGown
Keyword(s):  
Bacterial Spore ◽  
Spore Detection

In-situ silica nanoparticle assembly technique to develop an omniphobic membrane for durable membrane distillation

Desalination ◽  
2021 ◽  
Vol 499 ◽  
pp. 114832
Author(s):  
Yuanlu Xu ◽  
Yi Yang ◽  
Xinfei Fan ◽  
Zhijian Liu ◽  
Yongxin Song ◽  
...  
Keyword(s):  
Silica Nanoparticle ◽  
Membrane Distillation ◽  
Nanoparticle Assembly ◽  
In Situ Silica ◽  
Assembly Technique

In Situ Silica Nanoparticle Formation in a Rubber Matrix Monitored via Real-Time SAXS and Solid-State NMR Spectroscopy

2014 ◽  
Vol 47 (15) ◽  
pp. 5174-5185 ◽  
Author(s):  
Elena Miloskovska ◽  
Michael Ryan Hansen ◽  
Cornelius Friedrich ◽  
Denka Hristova-Bogaerds ◽  
Martin van Duin ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Real Time ◽  
Solid State Nmr ◽  
Silica Nanoparticle ◽  
Rubber Matrix ◽  
Nanoparticle Formation ◽  
Solid State Nmr Spectroscopy ◽  
In Situ Silica

scholarly journals Functional Tomographic Fluorescence Imaging of pH Microenvironments in Microbial Biofilms by Use of Silica Nanoparticle Sensors

2009 ◽  
Vol 75 (23) ◽  
pp. 7426-7435 ◽  
Author(s):  
Gabriela Hidalgo ◽  
Andrew Burns ◽  
Erik Herz ◽  
Anthony G. Hay ◽  
Paul L. Houston ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Tricarboxylic Acid Cycle ◽  
Three Dimensional ◽  
Silica Nanoparticle ◽  
In Situ Characterization ◽  
Local Conditions ◽  
Glucose Addition ◽  
Microbial Biofilms

ABSTRACT Attached bacterial communities can generate three-dimensional (3D) physicochemical gradients that create microenvironments where local conditions are substantially different from those in the surrounding solution. Given their ubiquity in nature and their impacts on issues ranging from water quality to human health, better tools for understanding biofilms and the gradients they create are needed. Here we demonstrate the use of functional tomographic imaging via confocal fluorescence microscopy of ratiometric core-shell silica nanoparticle sensors (C dot sensors) to study the morphology and temporal evolution of pH microenvironments in axenic Escherichia coli PHL628 and mixed-culture wastewater biofilms. Testing of 70-, 30-, and 10-nm-diameter sensor particles reveals a critical size for homogeneous biofilm staining, with only the 10-nm-diameter particles capable of successfully generating high-resolution maps of biofilm pH and distinct local heterogeneities. Our measurements revealed pH values that ranged from 5 to >7, confirming the heterogeneity of the pH profiles within these biofilms. pH was also analyzed following glucose addition to both suspended and attached cultures. In both cases, the pH became more acidic, likely due to glucose metabolism causing the release of tricarboxylic acid cycle acids and CO2. These studies demonstrate that the combination of 3D functional fluorescence imaging with well-designed nanoparticle sensors provides a powerful tool for in situ characterization of chemical microenvironments in complex biofilms.

scholarly journals In Situ Grafting of Silica Nanoparticle Precursors with Covalently Attached Bioactive Agents to Form PVA-Based Materials for Sustainable Active Packaging

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2889
Author(s):  
Miri Klein ◽  
Anat Molad Filossof ◽  
Idan Ashur ◽  
Sefi Vernick ◽  
Michal Natan-Warhaftig ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silica Nanoparticle ◽  
Listeria Innocua ◽  
Packaging Materials ◽  
Biodegradable Films ◽  
Log Reduction ◽  
High Antioxidant Activity ◽  
Bioactive Agents ◽  
Antibacterial And Antioxidant Activity

Sustainable antibacterial–antioxidant films were prepared using in situ graftings of silica nanoparticle (SNP) precursors with covalently attached bioactive agents benzoic acid (ba) or curcumin (cur) on polyvinyl alcohol (PVA). The modified PVA-SNP, PVA-SNP-ba and PVA-SNP-cur films were characterized using spectroscopic, physicochemical and microscopic methods. The prepared films showed excellent antibacterial and antioxidant activity, and increased hydrophobicity providing protection from undesired moisture. The PVA-SNP-ba films completely prevented the growth of the foodborne human pathogen Listeria innocua, whereas PVA-SNP-cur resulted in a 2.5 log reduction of this bacteria. The PVA-SNP-cur and PVA-SNP-ba films showed high antioxidant activity of 15.9 and 14.7 Mm/g TEAC, respectively. The described approach can serve as a generic platform for the formation of PVA-based packaging materials with tailor-made activity tuned by active substituents on silica precursors. Application of such biodegradable films bearing safe bioactive agents can be particularly valuable for advanced sustainable packaging materials in food and medicine.

scholarly journals Studies on a Biosynthethic Site in Dipicolinic Acid Formation by Bacterial Spore Formers

1967 ◽  
Vol 22 (9) ◽  
pp. 505-509
Author(s):  
Chikataro KAWASAKI ◽  
Masaomi KONDO ◽  
Jun SAKURAI
Keyword(s):  
Dipicolinic Acid ◽  
Bacterial Spore ◽  
Acid Formation

scholarly journals Printing spongy all-in-liquid materials

2021 ◽  
Author(s):  
Parisa Bazazi ◽  
Howard Stone ◽  
S. Hossein Hejazi
Keyword(s):  
3D Structure ◽  
Silica Nanoparticle ◽  
Simple Approach ◽  
Scaling Analysis ◽  
Nanoparticle Dispersions ◽  
Spongy Texture ◽  
Liquid Systems ◽  
Flexible Walls ◽  
Oil Water

Abstract Printing structured networks of functionalized droplets in a liquid medium enables engineering collectives of living cells for functional purposes [1, 2], bacterial ecology [3], and promises enormous applications in processes ranging from energy storage [4, 5] to drug delivery [6, 7]and tissue engineering [8]. Current approaches are limited to drop-by-drop printing [1, 2] or face limitations in reproducing the sophisticated internal features of a structured material and its interactions with the surrounding media [6, 9–11]. Here, we report on a simple approach for creating stable liquid filaments of silica nanoparticle dispersions and use them as inks to print all-in-liquid materials that consist of a network of droplets. Silica nanoparticles stabilize liquid filaments at Weber numbers two orders of magnitude smaller than previously reported in liquid-liquid systems by rapidly producing a concentrated microemulsion zone at the oil-water interface. We experimentally demonstrate that the printed aqueous phase is emulsified in-situ; consequently, a 3D structure is achieved with flexible walls consisting of layered microemulsions. The tube-like printed features have a spongy texture resembling miniaturized versions of “tube sponges” found in the oceans. A scaling analysis based on the interplay between hydro-dynamics and emulsification kinetics reveals that liquid filaments are formed when emulsions are generated and remain at the interface during the printing period. We demonstrate the utilization of filaments of the nanoparticle dispersions for printing fluidic channels and propose to use them as lab-on-a-chip devices.

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