Direct high-pressure NMR observation of dipicolinic acid leaking from bacterial spore: A crucial step for thermal inactivation

2017 ◽  
Vol 231 ◽  
pp. 10-14 ◽  
Author(s):  
Kazuyuki Akasaka ◽  
Akihiro Maeno ◽  
Akira Yamazaki
Keyword(s):  
High Pressure ◽  
Thermal Inactivation ◽  
Dipicolinic Acid ◽  
Bacterial Spore ◽  
Crucial Step

scholarly journals Analysis for bacterial spore-specific dipicolinic acid by high-pressure liquid chromatography.

1988 ◽  
Vol 43 (5) ◽  
pp. 927-930 ◽  
Author(s):  
Kazuhito WATABE ◽  
Kyoko MAEKAWA ◽  
Atsumi YAMADA ◽  
Tomihiko KOSHIKAWA ◽  
Setsuko OGAWA ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Dipicolinic Acid ◽  
Bacterial Spore

scholarly journals Bacillus spores: a review of their properties and inactivation processing technologies

2020 ◽  
Vol 29 (11) ◽  
pp. 1447-1461
Author(s):  
Won-Il Cho ◽  
Myong-Soo Chung
Keyword(s):  
High Pressure ◽  
Electric Fields ◽  
Antimicrobial Agents ◽  
Thermal Inactivation ◽  
Dipicolinic Acid ◽  
Divalent Cations ◽  
Combined Treatment ◽  
Processing Technologies ◽  
Cold Plasmas ◽  
Bacillus Spores

Abstract Many factors determine the resistance properties of a Bacillus spore to heat, chemical and physical processing, including thick proteinaceous coats, peptidoglycan cortex and low water content, high levels of dipicolinic acid (DPA), and divalent cations in the spore core. Recently, attention has been focused on non-thermal inactivation methods based on high pressure, ultrasonic, high voltage electric fields and cold plasmas for inactivating Bacillus spores associated with deterioration in quality and safety. The important chemical sporicides are glutaraldehyde, chorine-releasing agents, peroxygens, and ethylene oxide. Some food-grade antimicrobial agents exhibit sporostatic and sporicidal activities, such as protamine, polylysine, sodium lactate, essential oils. Surfactants with hydrophilic and hydrophobic properties have been reported to have inactivation activity against spores. The combined treatment of physical and chemical treatment such as heating, UHP (ultra high pressure), PEF (pulsed electric field), UV (ultraviolet), IPL (intense pulsed light) and natural antimicrobial agents can act synergistically and effectively to kill Bacillus spores in the food industry.

In Situ Surface-Etched Bacterial Spore Detection Using Dipicolinic Acid–Europium–Silica Nanoparticle Bioreporters

2011 ◽  
Vol 65 (8) ◽  
pp. 866-875 ◽  
Author(s):  
Clint B. Smith ◽  
John E. Anderson ◽  
Jarrod D. Edwards ◽  
Kinson C. Kam
Keyword(s):  
Dipicolinic Acid ◽  
Silica Nanoparticle ◽  
Bacterial Spore ◽  
Spore Detection

scholarly journals Studies on a Biosynthethic Site in Dipicolinic Acid Formation by Bacterial Spore Formers

1967 ◽  
Vol 22 (9) ◽  
pp. 505-509
Author(s):  
Chikataro KAWASAKI ◽  
Masaomi KONDO ◽  
Jun SAKURAI
Keyword(s):  
Dipicolinic Acid ◽  
Bacterial Spore ◽  
Acid Formation

scholarly journals High-pressure stabilization of α-chymotrypsin entrapped in reversed micelles of aerosol OT in octane against thermal inactivation

FEBS Letters ◽  
1995 ◽  
Vol 364 (1) ◽  
pp. 98-100 ◽  
Author(s):  
Roman V. Rariy ◽  
Nicole Bec ◽  
J.-L. Saldana ◽  
Sergey N. Nametkin ◽  
Vadim V. Mozhaev ◽  
...  
Keyword(s):  
High Pressure ◽  
Thermal Inactivation ◽  
Reversed Micelles ◽  
Aerosol Ot ◽  
Pressure Stabilization

Bacterial spore inactivation by ultra-high pressure homogenization

2014 ◽  
Vol 26 ◽  
pp. 116-123 ◽  
Author(s):  
E. Georget ◽  
B. Miller ◽  
K. Aganovic ◽  
M. Callanan ◽  
V. Heinz ◽  
...  
Keyword(s):  
High Pressure ◽  
Bacterial Spore ◽  
High Pressure Homogenization ◽  
Ultra High Pressure

scholarly journals Mechanisms of Induction of Germination of Bacillus subtilis Spores by High Pressure

2002 ◽  
Vol 68 (6) ◽  
pp. 3172-3175 ◽  
Author(s):  
Madan Paidhungat ◽  
Barbara Setlow ◽  
William B. Daniels ◽  
Dallas Hoover ◽  
Efstathia Papafragkou ◽  
...  
Keyword(s):  
High Pressure ◽  
Bacillus Subtilis ◽  
Spore Germination ◽  
Dipicolinic Acid ◽  
Wild Type ◽  
Lytic Enzymes

ABSTRACT Spores of Bacillus subtilis lacking all germinant receptors germinate >500-fold slower than wild-type spores in nutrients and were not induced to germinate by a pressure of 100 MPa. However, a pressure of 550 MPa induced germination of spores lacking all germinant receptors as well as of receptorless spores lacking either of the two lytic enzymes essential for cortex hydrolysis during germination. Complete germination of spores either lacking both cortex-lytic enzymes or with a cortex not attacked by these enzymes was not induced by a pressure of 550 MPa, but treatment of these mutant spores with this pressure caused the release of dipicolinic acid. These data suggest the following conclusions: (i) a pressure of 100 MPa induces spore germination by activating the germinant receptors; and (ii) a pressure of 550 MPa opens channels for release of dipicolinic acid from the spore core, which leads to the later steps in spore germination.

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