Hand-held syringe as a portable plastic pump for on-chip continuous-flow PCR: miniaturization of sample injection device

The Analyst ◽  
2012 ◽  
Vol 137 (4) ◽  
pp. 983-990 ◽  
Author(s):  
Wenming Wu ◽  
Kieu The Loan Trinh ◽  
Nae Yoon Lee
Keyword(s):  
Continuous Flow ◽  
Injection Device ◽  
Sample Injection ◽  
On Chip

Flow stabilizer on a syringe tip for hand-powered microfluidic sample injection

Lab on a Chip ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 214-222 ◽  
Author(s):  
Nan Xiang ◽  
Yu Han ◽  
Yuan Jia ◽  
Zhiguo Shi ◽  
Hong Yi ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Sample Injection ◽  
Resource Poor ◽  
Flow Stabilizer

We proposed a novel syringe flow-stabilizer for hand-powered, precise, continuous-flow microfluidic sample injection in resource-poor settings.

The Design of a Continuous Flow Combinatorial Screening Micro Reactor System with On-Chip Detection

2000 ◽  
pp. 59-62
Author(s):  
Victoria Skelton ◽  
Gillian Greenway ◽  
Stephen Haswell ◽  
Peter Styring ◽  
David Morgan ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Reactor System ◽  
Combinatorial Screening ◽  
On Chip ◽  
Micro Reactor

scholarly journals Microfluidic System for Observation of Bacterial Culture and Effects on Biofilm Formation at Microscale

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 606 ◽  
Author(s):  
Xiao-Yan Zhang ◽  
Kai Sun ◽  
Aliya Abulimiti ◽  
Pian-Pian Xu ◽  
Zhe-Yu Li
Keyword(s):  
Membrane Fouling ◽  
Continuous Flow ◽  
Biofilm Formation ◽  
Natural World ◽  
Microfluidic System ◽  
Fluorescence Labeling ◽  
In Situ Observation ◽  
Time Observation ◽  
On Chip ◽  
Bacteria Culture

Biofilms exist in the natural world and applied to many industries. However, due to the variety of characteristics caused by their complex components, biofilms can also lead to membrane fouling and recurrent infections which pose threats to human health. So, to make the best use of their advantages and avoid their disadvantages, knowing the best time and methods for improving or preventing biofilm formation is important. In situ observation without fluorescence labeling in microscale and according to a time scale is useful to research biofilm and confine its formation. In this study, we developed a microfluidic system for real-time observation of bacteria culture and biofilms development at microscale. We cultured E. coli ATCC 25922 on a chip at continuous flow of the velocity, which could promote bacterial formation. Biofilms formation under the condition of adding amoxicillin at different times is also discussed. In addition, the mixed strains from sludge were also cultured on chip, and possible factors in biofilm formation are discussed. Our results show that a microfluidic device could culture microorganisms in continuous flow and accelerate them to adhere to the surface, thereby promoting biofilm formation. Overall, this platform is a useful tool in research on initial biofilm formation, which can contribute to preventing biofouling and infections.

Pressure-Driven Sample Injection with Quantitative Liquid Dispensing for On-Chip Electrophoresis

2004 ◽  
Vol 20 (3) ◽  
pp. 483-487 ◽  
Author(s):  
Nae Yoon LEE ◽  
Masumi YAMADA ◽  
Minoru SEKI
Keyword(s):  
Sample Injection ◽  
On Chip ◽  
Pressure Driven

Photonic lab-on-chip (PhLOC) for enzyme-catalyzed reactions in continuous flow

2014 ◽  
Vol 18 (5-6) ◽  
pp. 1277-1286 ◽  
Author(s):  
Isaac Rodríguez-Ruiz ◽  
Eduard Masvidal-Codina ◽  
Tobias N. Ackermann ◽  
Andreu Llobera
Keyword(s):  
Continuous Flow ◽  
Lab On Chip ◽  
Catalyzed Reactions ◽  
On Chip ◽  
Enzyme Catalyzed Reactions

Continuous-Flow Chemical Processing in Three-Dimensional Microchannel Network for On-Chip Integration of Multiple Reactions in a Combinatorial Mode

2005 ◽  
Vol 24 (6) ◽  
pp. 742-757 ◽  
Author(s):  
Yoshikuni Kikutani ◽  
Masaharu Ueno ◽  
Hideaki Hisamoto ◽  
Manabu Tokeshi ◽  
Takehiko Kitamori
Keyword(s):  
Continuous Flow ◽  
Three Dimensional ◽  
Chemical Processing ◽  
Multiple Reactions ◽  
On Chip
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