Amino poly(glycerol methacrylate)s for oligonucleic acid delivery with enhanced transfection efficiency and low cytotoxicity

Soft Matter ◽  
2011 ◽  
Vol 7 (19) ◽  
pp. 9239 ◽  
Author(s):  
Hui Gao ◽  
Xueyou Lu ◽  
Yanan Ma ◽  
Yingwei Yang ◽  
Jianfa Li ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Low Cytotoxicity

Bio-inspired gene carriers with low cytotoxicity constructed via the assembly of dextran nanogels and nano-coacervates

Nanomedicine ◽  
2020 ◽  
Vol 15 (13) ◽  
pp. 1285-1296 ◽  
Author(s):  
Chenglong Wang ◽  
Jiayi You ◽  
Miaomiao Gao ◽  
Peipei Zhang ◽  
Guoxiong Xu ◽  
...  
Keyword(s):  
Positive Charge ◽  
Transfection Efficiency ◽  
Gene Carrier ◽  
Gene Carriers ◽  
Environmentally Responsive ◽  
Potential Applications ◽  
Internal Peptide ◽  
The Common ◽  
Low Cytotoxicity ◽  
Peptide Gene

Aim: To achieve safe and biocompatible gene carriers. Materials & methods: A core/shell-structured hierarchical carrier with an internal peptide/gene coacervate ‘core’ and a dextran nanogel ‘shell’ on the surface has been designed. Results: The dextran nanogels shield coacervate (DNSC) can effectively condense genes and release them in reducing environments. The dextran nanogel-based ‘shell’ can effectively shield the positive charge of the peptide/gene coacervate ‘core’, thus reducing the side effects of cationic gene carriers. In contrast with the common nonviral gene carriers that had high cytotoxicities, the DNSC showed a high transfection efficiency while maintaining a low cytotoxicity. Conclusion: The DNSC provides an effective environmentally responsive gene carrier with potential applications in the fields of gene therapy and gene carrier development.

scholarly journals The Development of Functional Non-Viral Vectors for Gene Delivery

2019 ◽  
Vol 20 (21) ◽  
pp. 5491 ◽  
Author(s):  
Patil ◽  
Gao ◽  
Lin ◽  
Li ◽  
Dang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Gene Vectors ◽  
Potential Applications ◽  
Low Toxicity ◽  
Low Cytotoxicity

Gene therapy is manipulation in/of gene expression in specific cells/tissue to treat diseases. This manipulation is carried out by introducing exogenous nucleic acids, such as DNA or RNA, into the cell. Because of their negative charge and considerable larger size, the delivery of these molecules, in general, should be mediated by gene vectors. Non-viral vectors, as promising delivery systems, have received considerable attention due to their low cytotoxicity and non-immunogenicity. As research continued, more and more functional non-viral vectors have emerged. They not only have the ability to deliver a gene into the cells but also have other functions, such as the performance of fluorescence imaging, which aids in monitoring their progress, targeted delivery, and biodegradation. Recently, many reviews related to non-viral vectors, such as polymers and cationic lipids, have been reported. However, there are few reviews regarding functional non-viral vectors. This review summarizes the common functional non-viral vectors developed in the last ten years and their potential applications in the future. The transfection efficiency and the transport mechanism of these materials were also discussed in detail. We hope that this review can help researchers design more new high-efficiency and low-toxicity multifunctional non-viral vectors, and further accelerate the progress of gene therapy.

Black phosphorus quantum dot based novel siRNA delivery systems in human pluripotent teratoma PA-1 cells

2017 ◽  
Vol 5 (27) ◽  
pp. 5433-5440 ◽  
Author(s):  
Feng Yin ◽  
Kuan Hu ◽  
Si Chen ◽  
Dongyuan Wang ◽  
Jianing Zhang ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Quantum Dot ◽  
Transfection Efficiency ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Black Phosphorus ◽  
Semiconducting Materials ◽  
Photothermal Effects ◽  
Low Cytotoxicity

As a novel semiconducting materials, BP-QDs possess superior transfection efficiency, excellent biocompatibility and low cytotoxicity, which shows promising potential for siRNA delivery and photothermal effects in cancer therapy.

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

2016 ◽  
Vol 4 (39) ◽  
pp. 6462-6467 ◽  
Author(s):  
Sheng-Gang Ding ◽  
Lei Yu ◽  
Long-Hai Wang ◽  
Lin-Ding Wang ◽  
Zhi-Qiang Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Dendritic Polymer ◽  
Self Assembling ◽  
High Gene ◽  
Low Cytotoxicity

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery.

PEGylated Plier-Like Cationic Niosomes on Gene Delivery in HeLa Cells

2019 ◽  
Vol 819 ◽  
pp. 151-156
Author(s):  
Supusson Pengnam ◽  
Samarwadee Plianwong ◽  
Kanokwan Singpanna ◽  
Nattisa Ni-yomtham ◽  
Widchaya Radchatawedchakoon ◽  
...  
Keyword(s):  
Particle Size ◽  
Gene Delivery ◽  
Hela Cells ◽  
Transfection Efficiency ◽  
Cationic Lipid ◽  
Dna Delivery ◽  
Molar Ratio ◽  
The Novel ◽  
Low Cytotoxicity ◽  
Cationic Niosomes

Lipid-based formulations have been used as a widespread carrier to improve gene delivery. Niosomes, one type of lipid-based vesicular systems are produced from non-ionic surfactants which are generally inexpensive and potentially more stable than phospholipids. This article was to develop PEGylated cationic niosomes for DNA delivery. Thin film hydration and sonication method were applied for cationic niosomes. The niosome formulations were composed of Span 20, cholesterol (Chol) and plier-like cationic lipid B (PCL-B) with or without cholesterol-polyethylene glycol 2000 (Chol-PEG). The physicochemical properties of cationic niosomes and nioplexes were evaluated including particle size, zeta potential, DNA condensation and serum protection. The transfection efficiency and cell viability were examined in HeLa cells. The particle size and surface charge of PEGylated cationic niosome containing Span 20: Chol: PCL-B: Chol-PEG at the molar ratio of 2.5: 2.5: 1.5: 0.14 (N-PEG2) were 129.47 ± 2.15 nm and 25.93 ± 4.18 mV, respectively. These PEGylated cationic niosomes could condense pDNA into the nanosize particles and also enhance the serum protection ability for at least 6 h. Moreover, N-PEG2 exhibited high transfection efficiency in comparison with lipofectamine® 2000 and low cytotoxicity. Therefore, the novel PEGylated cationic niosomes have the capability to develop as a promising potential carrier for DNA delivery.

Low molecular weight chitosan–protamine conjugate for siRNA delivery with enhanced stability and transfection efficiency

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110951-110963 ◽  
Author(s):  
Sushilkumar Patil ◽  
Priyanka Bhatt ◽  
Rohan Lalani ◽  
Jitendra Amrutiya ◽  
Imran Vhora ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Sirna Delivery ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan ◽  
Low Cytotoxicity ◽  
Enhanced Stability

Chitosan is among the few polymers with high biocompatibility and low cytotoxicity.

Development of new self-assembled cationic amino liposomes for efficient gene delivery

2020 ◽  
Vol 8 (11) ◽  
pp. 3021-3025 ◽  
Author(s):  
Yihang Wu ◽  
Yue Xiong ◽  
Ling Wang ◽  
Quanming Zhou ◽  
Linxian Li ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Self Assembling ◽  
Efficient Gene ◽  
Self Assembled ◽  
Low Cytotoxicity

A library of lipidoids self-assembling to liposomes exhibits excellent transfection efficiency in HEK 293T cells and mESCs with low cytotoxicity.

Acid-sensitive poly(β-cyclodextrin)-based multifunctional supramolecular gene vector

2018 ◽  
Vol 9 (4) ◽  
pp. 450-462 ◽  
Author(s):  
Yunti Zhang ◽  
Qimin Jiang ◽  
Marcin Wojnilowicz ◽  
Shuaijun Pan ◽  
Yi Ju ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Vector ◽  
Low Cytotoxicity

Multifunctional host–guest supramolecular PCD-acetal-PGEA/Ad-PEG-FA polyplexes showing FA-targeting and acid-triggered intracellular gene release resulted in good transfection efficiency and low cytotoxicity.

Hyaluronic acid–siRNA conjugates complexed with cationic solid lipid nanoparticles for target specific gene silencing

RSC Advances ◽  
2014 ◽  
Vol 4 (37) ◽  
pp. 19338-19344 ◽  
Author(s):  
Min-Young Lee ◽  
Won Ho Kong ◽  
Ho Sang Jung ◽  
Sei Kwang Hahn
Keyword(s):  
Hyaluronic Acid ◽  
Gene Silencing ◽  
Solid Lipid Nanoparticles ◽  
Transfection Efficiency ◽  
Lipid Nanoparticles ◽  
Specific Gene ◽  
Solid Lipid ◽  
Dual Targeting ◽  
Low Cytotoxicity ◽  
Targeting Effect

The reducible hyaluronic acid (HA)–siRNA conjugates complexed with cationic solid lipid nanoparticles (CSLNs) with low cytotoxicity and high transfection efficiency was successfully developed for effective liver-specific gene silencing by the dual targeting effect of HA and CSLN.

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