Development of new self-assembled cationic amino liposomes for efficient gene delivery

2020 ◽  
Vol 8 (11) ◽  
pp. 3021-3025 ◽  
Author(s):  
Yihang Wu ◽  
Yue Xiong ◽  
Ling Wang ◽  
Quanming Zhou ◽  
Linxian Li ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Self Assembling ◽  
Efficient Gene ◽  
Self Assembled ◽  
Low Cytotoxicity

A library of lipidoids self-assembling to liposomes exhibits excellent transfection efficiency in HEK 293T cells and mESCs with low cytotoxicity.

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

2016 ◽  
Vol 4 (39) ◽  
pp. 6462-6467 ◽  
Author(s):  
Sheng-Gang Ding ◽  
Lei Yu ◽  
Long-Hai Wang ◽  
Lin-Ding Wang ◽  
Zhi-Qiang Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Dendritic Polymer ◽  
Self Assembling ◽  
High Gene ◽  
Low Cytotoxicity

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery.

scholarly journals Aromatic Thioacetal-Bridged ROS-Responsive Nanoparticles as Novel Gene Delivery Vehicles

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2061 ◽  
Author(s):  
Guo-Qing Lin ◽  
Wen-Jing Yi ◽  
Qiang Liu ◽  
Xue-Jun Yang ◽  
Zhi-Gang Zhao
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Zeta Potentials ◽  
Sar Studies ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Delivery Vehicles ◽  
Low Cytotoxicity

In this report, a series of polycations are designed and synthesized by conjugating reactive oxygen species (ROS)-responsive thioacetal-linkers to low molecular weight (LMW) polyethylenimine (PEI) via ring-opening polymerization. Their structure–activity relationships (SARs) as gene delivery vectors are systematically studied. Although the MWs of the target polymers are only ~9 KDa, they show good DNA binding ability. The formed polyplexes, which are stable toward serum but decomposed under ROS-conditions, have appropriate sizes (180~300 nm) and positive zeta-potentials (+35~50 mV). In vitro experiments reveal that these materials have low cytotoxicity, and higher transfection efficiency (TE) than controls. Furthermore, the title polymers exhibit excellent serum tolerance. With the present of 10% serum, the TE of the polymers even increases up to 10 times higher than 25 KDa PEI and 9 times higher than Lipofectamine 2000. The SAR studies also reveal that electron-withdrawing groups on the aromatic ring in 4a may benefit to balance between the DNA condensation and release for efficient gene transfection.

Guanidine-rich helical polypeptides bearing hydrophobic amino acid pendants for efficient gene delivery

2021 ◽  
Author(s):  
Zikun Yu ◽  
Zhimin Zhang ◽  
Jing Yan ◽  
Ziyin Zhao ◽  
Chenglong Ge ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Amino Acid ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Hydrophobic Amino Acid ◽  
High Transfection Efficiency ◽  
Efficient Gene ◽  
Low Cytotoxicity

Guanidine-rich helical polypeptides bearing hydrophobic amino acid pendants displayed high transfection efficiency both in vitro and in vivo and low cytotoxicity toward applications in gene therapy.

Design and synthesis of a fluorescent amino poly(glycidyl methacrylate) for efficient gene delivery

2019 ◽  
Vol 7 (11) ◽  
pp. 1875-1881 ◽  
Author(s):  
Hongtao Chen ◽  
Shusen You ◽  
Qing Cai ◽  
Yang Zheng ◽  
Liqun Zhang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Glycidyl Methacrylate ◽  
Dna Delivery ◽  
Atom Transfer ◽  
Design And Synthesis ◽  
Efficient Gene ◽  
Low Cytotoxicity ◽  
Delivery Efficiency

A fluorescent amino poly(glycidyl methacrylate) (PGOHMA) was synthesized by atom transfer radical polymerization (ATRP) and post-polymerization. PGOHMA has low cytotoxicity and high DNA delivery efficiency.

A dendritic catiomer with an MOF motif for the construction of safe and efficient gene delivery systems

2017 ◽  
Vol 5 (42) ◽  
pp. 8322-8329 ◽  
Author(s):  
Shuqi Dong ◽  
Qixian Chen ◽  
Wei Li ◽  
Zhu Jiang ◽  
Jianbiao Ma ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gold Standard ◽  
Transfection Efficiency ◽  
A549 Cells ◽  
Delivery Systems ◽  
The Core ◽  
Efficient Gene

The dendritic catiomer using biocompatible Zr-MOFs as the core exhibited a markedly higher transfection efficiency and lower cytotoxicity than the commercial gold standard branched PEI25k in A549 cells.

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

Tuning the Self-Assembling of Pyridinium Cationic Lipids for Efficient Gene Delivery into Neuronal Cells

2013 ◽  
Vol 14 (8) ◽  
pp. 2750-2764 ◽  
Author(s):  
Sushma Savarala ◽  
Eugen Brailoiu ◽  
Stephanie L. Wunder ◽  
Marc A. Ilies
Keyword(s):  
Gene Delivery ◽  
Neuronal Cells ◽  
Cationic Lipids ◽  
The Self ◽  
Self Assembling ◽  
Efficient Gene

Peptide amphiphiles with multifunctional fragments promoting cellular uptake and endosomal escape as efficient gene vectors

2015 ◽  
Vol 3 (6) ◽  
pp. 1068-1078 ◽  
Author(s):  
Liang Luan ◽  
Qingbin Meng ◽  
Liang Xu ◽  
Zhao Meng ◽  
Husheng Yan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cellular Uptake ◽  
Transfection Efficiency ◽  
Endosomal Escape ◽  
Peptide Amphiphiles ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Gene Vectors ◽  
Lipofectamine 2000

A series of peptides containing multiple functional fragments were designed as gene-delivery vectors with transfection efficiency comparable to Lipofectamine 2000.

PEGylated Plier-Like Cationic Niosomes on Gene Delivery in HeLa Cells

2019 ◽  
Vol 819 ◽  
pp. 151-156
Author(s):  
Supusson Pengnam ◽  
Samarwadee Plianwong ◽  
Kanokwan Singpanna ◽  
Nattisa Ni-yomtham ◽  
Widchaya Radchatawedchakoon ◽  
...  
Keyword(s):  
Particle Size ◽  
Gene Delivery ◽  
Hela Cells ◽  
Transfection Efficiency ◽  
Cationic Lipid ◽  
Dna Delivery ◽  
Molar Ratio ◽  
The Novel ◽  
Low Cytotoxicity ◽  
Cationic Niosomes

Lipid-based formulations have been used as a widespread carrier to improve gene delivery. Niosomes, one type of lipid-based vesicular systems are produced from non-ionic surfactants which are generally inexpensive and potentially more stable than phospholipids. This article was to develop PEGylated cationic niosomes for DNA delivery. Thin film hydration and sonication method were applied for cationic niosomes. The niosome formulations were composed of Span 20, cholesterol (Chol) and plier-like cationic lipid B (PCL-B) with or without cholesterol-polyethylene glycol 2000 (Chol-PEG). The physicochemical properties of cationic niosomes and nioplexes were evaluated including particle size, zeta potential, DNA condensation and serum protection. The transfection efficiency and cell viability were examined in HeLa cells. The particle size and surface charge of PEGylated cationic niosome containing Span 20: Chol: PCL-B: Chol-PEG at the molar ratio of 2.5: 2.5: 1.5: 0.14 (N-PEG2) were 129.47 ± 2.15 nm and 25.93 ± 4.18 mV, respectively. These PEGylated cationic niosomes could condense pDNA into the nanosize particles and also enhance the serum protection ability for at least 6 h. Moreover, N-PEG2 exhibited high transfection efficiency in comparison with lipofectamine® 2000 and low cytotoxicity. Therefore, the novel PEGylated cationic niosomes have the capability to develop as a promising potential carrier for DNA delivery.

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