Diamond nanowires for highly sensitive matrix-free mass spectrometry analysis of small molecules

Nanoscale ◽  
2012 ◽  
Vol 4 (1) ◽  
pp. 231-238 ◽  
Author(s):  
Yannick Coffinier ◽  
Sabine Szunerits ◽  
Hervé Drobecq ◽  
Oleg Melnyk ◽  
Rabah Boukherroub
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Highly Sensitive ◽  
Matrix Free

Highly sensitive protein detection by combination of atomic force microscopy fishing with charge generation and mass spectrometry analysis

FEBS Journal ◽  
2014 ◽  
Vol 281 (20) ◽  
pp. 4705-4717 ◽  
Author(s):  
Yuri D. Ivanov ◽  
Tatyana Pleshakova ◽  
Krystina Malsagova ◽  
Andrey Kozlov ◽  
Anna Kaysheva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Protein Detection ◽  
Mass Spectrometry Analysis ◽  
Charge Generation ◽  
Spectrometry Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Highly Sensitive

High sensitive matrix-free mass spectrometry analysis of peptides using silicon nanowires-based digital microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (9) ◽  
pp. 1620 ◽  
Author(s):  
Florian Lapierre ◽  
Gaëlle Piret ◽  
Hervé Drobecq ◽  
Oleg Melnyk ◽  
Yannick Coffinier ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Microfluidic Device ◽  
Silicon Nanowires ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Digital Microfluidic ◽  
Matrix Free

Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression

2014 ◽  
Vol 112 (12) ◽  
pp. 1230-1243 ◽  
Author(s):  
Alzbeta Davidova ◽  
Matthias Mörgelin ◽  
Adam Linder ◽  
Michael Larsen ◽  
Klaus Qvortrup ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Infectious Diseases ◽  
Polymorphonuclear Neutrophils ◽  
Mass Spectrometry Analysis ◽  
Selected Reaction Monitoring ◽  
Abundance Pattern ◽  
Spectrometry Analysis ◽  
Sepsis Diagnosis ◽  
Highly Sensitive ◽  
Novel Biomarkers

SummaryEarly diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation, an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil- derived protein abundance pattern in blood plasma consisting of 20 proteins that can be used as a protein signature for severe infectious diseases. Our results also show that SRM is highly sensitive, specific, and reproducible and, thus, a promising technology to study a complex, dynamic and multifactorial disease such as sepsis.

3D printing of graphene-doped target for “matrix-free” laser desorption/ionization mass spectrometry

2018 ◽  
Vol 54 (22) ◽  
pp. 2723-2726 ◽  
Author(s):  
Dingyi Wang ◽  
Xiu Huang ◽  
Jie Li ◽  
Bin He ◽  
Qian Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
3D Printing ◽  
Laser Desorption ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Spectrometry Analysis ◽  
Printing Technique ◽  
Matrix Free ◽  
3D Printing Technique

3D printing technique was used to easily fabricate graphene-doped target that permitted the LDI-TOF mass spectrometry analysis without adding matrix.

Simple Synthesis of Fluorinated Ene-Ynes via In Situ Generation of Allenes

Synthesis ◽  
2021 ◽  
Author(s):  
Ellen M. Sletten ◽  
Joseph A. Jaye
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Biologically Active ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Synthetic Route ◽  
Spectrometry Analysis ◽  
Complex Products ◽  
In Situ Generation

AbstractFluorination of small molecules is a key route toward modulating reactivity and bioactivity. The 1,3 ene-yne functionality is an important synthon towards complex products, as well as a common functionality in biologically active molecules. Here, we present a new synthetic route towards fluorinated ene-ynes from simple starting materials. We employ gas chromatography-mass spectrometry analysis to probe the sequential eliminations necessary for this transformation and observe an allene intermediate. The ene-yne products are sufficiently fluorous to enable purification via fluorous extraction. This methodology will allow facile access to functional, fluorous ene-ynes.

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