Highly sensitive protein detection by combination of atomic force microscopy fishing with charge generation and mass spectrometry analysis

FEBS Journal ◽  
2014 ◽  
Vol 281 (20) ◽  
pp. 4705-4717 ◽  
Author(s):  
Yuri D. Ivanov ◽  
Tatyana Pleshakova ◽  
Krystina Malsagova ◽  
Andrey Kozlov ◽  
Anna Kaysheva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Protein Detection ◽  
Mass Spectrometry Analysis ◽  
Charge Generation ◽  
Spectrometry Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Highly Sensitive

scholarly journals Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification

2015 ◽  
Vol 61 (3) ◽  
pp. 363-372 ◽  
Author(s):  
N.S. Bukharina ◽  
Yu.D. Ivanov ◽  
T.O. Pleshakova ◽  
P.A. Frantsuzov ◽  
E.Yu. Andreeva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Protein Detection ◽  
Target Protein ◽  
Mass Spectrometry Analysis ◽  
Molecular Probe ◽  
Bulk Solution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mass Spectrometry Identification

A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.

scholarly journals Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker

Minerals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 464
Author(s):  
Anastasia A. Valueva ◽  
Ivan D. Shumov ◽  
Anna L. Kaysheva ◽  
Irina A. Ivanova ◽  
Vadim S. Ziborov ◽  
...  
Keyword(s):  
Protein Immobilization ◽  
Mass Spectrometry Analysis ◽  
Biological Macromolecules ◽  
Spectrometry Analysis ◽  
Force Microscopy ◽  
Amino Silane ◽  
Research Areas ◽  
Atomic Force ◽  
Muscovite Mica ◽  
Peroxidase Enzyme

Muscovite mica with an amino silane-modified surface is commonly used as a substrate in atomic force microscopy (AFM) studies of biological macromolecules. Herein, the efficiency of two different protein immobilization strategies employing either (N-hydroxysuccinimide ester)-based crosslinker (DSP) or benzophenone-based photoactivatable crosslinker (SuccBB) has been compared using AFM and mass spectrometry analysis. Two proteins with different physicochemical properties—human serum albumin (HSA) and horseradish peroxidase enzyme protein (HRP)—have been used as model objects in the study. In the case of HRP, both crosslinkers exhibited high immobilization efficiency—as opposed to the case with HSA, when sufficient capturing efficiency has only been observed with SuccBB photocrosslinker. The results obtained herein can find their application in commonly employed bioanalytical systems and in the development of novel highly sensitive chip-based diagnostic platforms employing immobilized proteins. The obtained data can also be of interest for other research areas in medicine and biotechnology employing immobilized biomolecules.

Diamond nanowires for highly sensitive matrix-free mass spectrometry analysis of small molecules

Nanoscale ◽  
2012 ◽  
Vol 4 (1) ◽  
pp. 231-238 ◽  
Author(s):  
Yannick Coffinier ◽  
Sabine Szunerits ◽  
Hervé Drobecq ◽  
Oleg Melnyk ◽  
Rabah Boukherroub
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Highly Sensitive ◽  
Matrix Free

scholarly journals Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

2015 ◽  
pp. 1597 ◽  
Author(s):  
Ivan Shumov ◽  
Yuri Ivanov ◽  
Anna Kaysheva ◽  
Pavel Frantsuzov ◽  
Tatyana Pleshakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

scholarly journals Salmon Muscle Adherence to Polymer Coatings and Determination of Antibiotic Residues by Reversed-Phase High-Performance Liquid Chromatography Coupled to Selected Reaction Monitoring Mass Spectrometry, Atomic Force Microscopy, and Fourier Transform Infrared Spectroscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
E. Zumelzu ◽  
M. J. Wehrhahn ◽  
O. Muñoz ◽  
F. Rull
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Urea Solution ◽  
Antibiotic Residues ◽  
Force Microscopy ◽  
Atomic Force

The persistent adhesion of salmon muscle to food container walls after treatment with urea solution was observed. This work evaluated the diffusion of antibiotics from the salmon muscle to the polyethylene terephthalate (PET) coating protecting the electrolytic chromium coated steel (ECCS) plates. New aquaculture production systems employ antibiotics such as florfenicol, florfenicol amine, oxytetracycline, and erythromycin to control diseases. The introduction of antibiotics is a matter of concern regarding the effects on human health and biodiversity. It is important to determine their impact on the adhesion of postmortem salmon muscle to can walls and the surface and structural changes affecting the functionality of multilayers. This work characterized the changes occurring in the multilayer PET polymer and steel of containers by electron microscopy, 3D atomic force microscopy (3D-AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. A robust mass spectrometry methodology was employed to determine the presence of antibiotic residues. No evidence of antibiotics was observed on the protective coating in the range between 0.001 and 2.0 ng/mL; however, the presence of proteins, cholesterol, and alpha-carotene was detected. This in-depth profiling of the matrix-level elements is relevant for the use of adequate materials in the canning export industry.

scholarly journals Sub-micron proximal probe thermal desorption and laser mass spectrometry on painting cross-sections

2014 ◽  
Vol 6 (22) ◽  
pp. 8940-8945 ◽  
Author(s):  
Shawn C. Owens ◽  
Jacob A. Berenbeim ◽  
Catherine Schmidt Patterson ◽  
Eoghan P. Dillon ◽  
M. S. de Vries
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Thermal Desorption ◽  
Cross Sections ◽  
Organic Dyes ◽  
Laser Mass Spectrometry ◽  
Force Microscopy ◽  
Atomic Force

Cross-sections containing organic dyes are used to demonstrate sub-micron atomic force microscopy thermal desorption (AFM-TD), followed by laser mass spectrometry.

scholarly journals Influence of a Thiolate Chemical Layer on GaAs (100) Biofunctionalization: An Original Approach Coupling Atomic Force Microscopy and Mass Spectrometry Methods

Materials ◽  
2013 ◽  
Vol 6 (11) ◽  
pp. 4946-4966 ◽  
Author(s):  
Alex Bienaime ◽  
Therese Leblois ◽  
Nicolas Gremaud ◽  
Maxime-Jean Chaudon ◽  
Marven Osta ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Atomic Force ◽  
Original Approach
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