High sensitive matrix-free mass spectrometry analysis of peptides using silicon nanowires-based digital microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (9) ◽  
pp. 1620 ◽  
Author(s):  
Florian Lapierre ◽  
Gaëlle Piret ◽  
Hervé Drobecq ◽  
Oleg Melnyk ◽  
Yannick Coffinier ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Microfluidic Device ◽  
Silicon Nanowires ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Digital Microfluidic ◽  
Matrix Free

Diamond nanowires for highly sensitive matrix-free mass spectrometry analysis of small molecules

Nanoscale ◽  
2012 ◽  
Vol 4 (1) ◽  
pp. 231-238 ◽  
Author(s):  
Yannick Coffinier ◽  
Sabine Szunerits ◽  
Hervé Drobecq ◽  
Oleg Melnyk ◽  
Rabah Boukherroub
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Highly Sensitive ◽  
Matrix Free

3D printing of graphene-doped target for “matrix-free” laser desorption/ionization mass spectrometry

2018 ◽  
Vol 54 (22) ◽  
pp. 2723-2726 ◽  
Author(s):  
Dingyi Wang ◽  
Xiu Huang ◽  
Jie Li ◽  
Bin He ◽  
Qian Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
3D Printing ◽  
Laser Desorption ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Spectrometry Analysis ◽  
Printing Technique ◽  
Matrix Free ◽  
3D Printing Technique

3D printing technique was used to easily fabricate graphene-doped target that permitted the LDI-TOF mass spectrometry analysis without adding matrix.

scholarly journals Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Takashi Kanamoto ◽  
Takashi Tachibana ◽  
Yasushi Kitaoka ◽  
Toshio Hisatomi ◽  
Yasuhiro Ikeda ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ocular Hypertension ◽  
Aspartic Acid ◽  
Atp Synthase ◽  
Wistar Rats ◽  
Protein Band ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Sds Page

Purpose. To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins. Methods. Adult Wistar rats with ocular hypertension were used as an experimental model. D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA. Results. D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats. Conclusion. Ocular hypertension affected the expression of proteins containing D-β-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.

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