Simultaneous Imaging and Chemical Attack of a Single Living Cell within a Confluent Cell Monolayer by Means of Scanning Electrochemical Microscopy

2011 ◽  
Vol 83 (1) ◽  
pp. 169-174 ◽  
Author(s):  
Stefan Bergner ◽  
Joachim Wegener ◽  
Frank-Michael Matysik
Keyword(s):  
Living Cell ◽  
Cell Monolayer ◽  
Scanning Electrochemical Microscopy ◽  
Simultaneous Imaging ◽  
Chemical Attack ◽  
Single Living Cell ◽  
Confluent Cell ◽  
Single Living ◽  
Electrochemical Microscopy

scholarly journals Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies

2021 ◽  
Author(s):  
Yuan Qiu ◽  
Chen-Chi Chien ◽  
Basilis Maroulis ◽  
Angelo Gaitas ◽  
Bin Gong
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Living Cell ◽  
Cell Monolayer ◽  
Extracellular Vesicle ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Living Cell ◽  
Single Living

Abstract In this article, a review of the application of atomic force microscopy (AFM) for the analyses of extracellular vesicles is presented. This information is then extended to include fluidic Atomic Force Microscopy (fluidic AFM) applications. Fluidic AFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. AFM applications involving single cell and extracellular vesicle studies, colloidal force spectroscopy, and single cell adhesion measurements are discussed. In this review, new results are offered, using fluidic AFM, to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces (LBFs) of endothelial or epithelial cells in a confluent cell monolayer in appropriate physiological environment, and (3) the ease of measurement of vertical binding force (VBFs) of intercellular adhesion between heterogeneous cells. Finally, key applications are discussed that include extracellular vesicle absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

An Integrated Electrochemical Nanodevice for Intracellular RNA Collection and Detection in Single Living Cell

2020 ◽  
Author(s):  
Hai-Yan Wang ◽  
Yi-Fan Ruan ◽  
Li-Bang Zhu ◽  
Xiao-Mei Shi ◽  
Wei-Wei Zhao ◽  
...  
Keyword(s):  
Living Cell ◽  
Single Living Cell ◽  
Single Living

scholarly journals Frontispiz: An Integrated Electrochemical Nanodevice for Intracellular RNA Collection and Detection in Single Living Cell

2021 ◽  
Vol 133 (24) ◽  
Author(s):  
Hai‐Yan Wang ◽  
Yi‐Fan Ruan ◽  
Li‐Bang Zhu ◽  
Xiao‐Mei Shi ◽  
Wei‐Wei Zhao ◽  
...  
Keyword(s):  
Living Cell ◽  
Single Living Cell ◽  
Single Living

Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell

2021 ◽  
Vol 175 ◽  
pp. 112915
Author(s):  
Yaran Chang ◽  
Yongjia Chen ◽  
Yunlong Shao ◽  
Boye Li ◽  
Yuanyuan Wu ◽  
...  
Keyword(s):  
Living Cell ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Single Living Cell ◽  
Single Living

scholarly journals Nanoelectrochemical analysis inside a single living cell

2020 ◽  
Vol 22 ◽  
pp. 94-101 ◽  
Author(s):  
Xinwei Zhang ◽  
Amir Hatamie ◽  
Andrew G. Ewing
Keyword(s):  
Living Cell ◽  
Single Living Cell ◽  
Single Living
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