Probing Cd2+-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy

2016 ◽  
Vol 120 (11) ◽  
pp. 6094-6103 ◽  
Author(s):  
Michelle S. M. Li ◽  
Fraser P. Filice ◽  
Jeffrey D. Henderson ◽  
Zhifeng Ding
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Scanning Electrochemical Microscopy ◽  
Live Cell ◽  
Cell Membrane Permeability ◽  
Redox Mediators ◽  
Electrochemical Microscopy

Concentrations dominated membrane permeability variation by fullerol nanoparticles on a single living HeLa cell

2016 ◽  
Vol 4 (34) ◽  
pp. 5755-5760
Author(s):  
Naiyun Liu ◽  
Mumei Han ◽  
Yalin Zhang ◽  
Xiuqin Wu ◽  
Juan Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Hela Cell ◽  
Membrane Permeability ◽  
Scanning Electrochemical Microscopy ◽  
Cell Membrane Permeability ◽  
Permeability Variation ◽  
Single Living ◽  
Electrochemical Microscopy

The effects in HeLa cell membrane permeability caused by the fullerenols C60(OH)n with different concentrations were studied by scanning electrochemical microscopy (SECM).

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

scholarly journals Biological Scanning Electrochemical Microscopy and Its Application to Live Cell Studies

2011 ◽  
Vol 83 (5) ◽  
pp. 1485-1492 ◽  
Author(s):  
Isabelle Beaulieu ◽  
Sabine Kuss ◽  
Janine Mauzeroll ◽  
Matthias Geissler
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Live Cell ◽  
Electrochemical Microscopy

Synthesis of novel cationic spermine-conjugated phosphotriester oligonucleotide for improvement of cell membrane permeability

2015 ◽  
Vol 25 (17) ◽  
pp. 3610-3615 ◽  
Author(s):  
Junsuke Hayashi ◽  
Tomoko Hamada ◽  
Ikumi Sasaki ◽  
Osamu Nakagawa ◽  
Shun-ichi Wada ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Cell Membrane Permeability

scholarly journals Red Cell Membrane Permeability Deduced from Bulk Diffusion Coefficients

1974 ◽  
Vol 64 (6) ◽  
pp. 706-729 ◽  
Author(s):  
W. R. Redwood ◽  
E. Rall ◽  
W. Perl
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Diffusion Coefficients ◽  
Bulk Diffusion ◽  
Red Cells ◽  
Cell Membrane Permeability ◽  
Red Cell ◽  
Cell Column ◽  
Red Cell Membrane ◽  
One Dimensional

The permeability coefficients of dog red cell membrane to tritiated water and to a series of[14C]amides have been deduced from bulk diffusion measurements through a "tissue" composed of packed red cells. Red cells were packed by centrifugation inside polyethylene tubing. The red cell column was pulsed at one end with radiolabeled solute and diffusion was allowed to proceed for several hours. The distribution of radioactivity along the red cell column was measured by sequential slicing and counting, and the diffusion coefficient was determined by a simple plotting technique, assuming a one-dimensional diffusional model. In order to derive the red cell membrane permeability coefficient from the bulk diffusion coefficient, the red cells were assumed to be packed in a regular manner approximating closely spaced parallelopipeds. The local steady-state diffusional flux was idealized as a one-dimensional intracellular pathway in parallel with a one-dimensional extracellular pathway with solute exchange occurring within the series pathway and between the pathways. The diffusion coefficients in the intracellular and extracellular pathways were estimated from bulk diffusion measurements through concentrated hemoglobin solutions and plasma, respectively; while the volume of the extracellular pathway was determined using radiolabeled sucrose. The membrane permeability coefficients were in satisfactory agreement with the data of Sha'afi, R. I., C. M. Gary-Bobo, and A. K. Solomon (1971. J. Gen. Physiol. 58:238) obtained by a rapid-reaction technique. The method is simple and particularly well suited for rapidly permeating solutes.

Non-contact-based determination of membrane permeability to water and dimethyl sulfoxide of cells virtually trapped in a self-induced micro-vortex

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Hsiu-Yang Tseng ◽  
Chiu-Jen Chen ◽  
Zong-Lin Wu ◽  
Yong-Ming Ye ◽  
Guo-Zhen Huang
Keyword(s):  
Cell Membrane ◽  
Dimethyl Sulfoxide ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Cell Type ◽  
Cryoprotective Agents ◽  
A Cell

Cell-membrane permeability to water (Lp) and cryoprotective agents (Ps) of a cell type is a crucial cellular information for achieving optimal cryopreservation in the biobanking industry. In this work, a...

scholarly journals Aluminum and Temperature Alteration of Cell Membrane Permeability of Quercus rubra

1991 ◽  
Vol 96 (2) ◽  
pp. 644-649 ◽  
Author(s):  
Junping Chen ◽  
Edward I. Sucoff ◽  
Eduard J. Stadelmann
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Quercus Rubra ◽  
Cell Membrane Permeability
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