Three-dimensional plotted PCL/β-TCP scaffolds coated with a collagen layer: preparation, physical properties and in vitro evaluation for bone tissue regeneration

2011 ◽  
Vol 21 (17) ◽  
pp. 6305 ◽  
Author(s):  
Hyeongjin Lee ◽  
GeunHyung Kim
Keyword(s):  
Physical Properties ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
In Vitro Evaluation ◽  
Collagen Layer

scholarly journals Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 61 ◽  
Author(s):  
Yannan Liu ◽  
Juan Gu ◽  
Daidi Fan
Keyword(s):  
Mechanical Properties ◽  
Enzymatic Hydrolysis ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Repair ◽  
Three Dimensional ◽  
High Strength ◽  
Biological Properties ◽  
Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

In vitro evaluation of simvastatin acid (SVA) coated beta-tricalcium phosphate (β-TCP) particle on bone tissue regeneration

2012 ◽  
Vol 20 (7) ◽  
pp. 754-761
Author(s):  
Dae Hyeok Yang ◽  
Min Soo Bae ◽  
Lingjuan Qiao ◽  
Dong Nyoung Heo ◽  
Jung Bok Lee ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Bone Tissue Regeneration ◽  
In Vitro Evaluation ◽  
Beta Tricalcium Phosphate ◽  
Simvastatin Acid

scholarly journals Synthesis of hybrid materials based polyester urethanes/ TiO2 and in vitro evaluation as potential biomaterial for bone tissue regeneration

2016 ◽  
Vol 4 ◽  
Author(s):  
Rodr�guez-Lorenzo Luis ◽  
Gonz�lez �Garc�a Dulce ◽  
Jim�nez-Gallegos Rodrigo ◽  
T�llez- Jurado Lucia ◽  
Mart�nez-Morales. Carmen
Keyword(s):  
Bone Tissue ◽  
Hybrid Materials ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
In Vitro Evaluation

scholarly journals Osteogenic Properties of 3D-Printed Silica-Carbon-Calcite Composite Scaffolds: Novel Approach for Personalized Bone Tissue Regeneration

2021 ◽  
Vol 22 (2) ◽  
pp. 475
Author(s):  
Parastoo Memarian ◽  
Francesco Sartor ◽  
Enrico Bernardo ◽  
Hamada Elsayed ◽  
Batur Ercan ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
Hemolysis Assay ◽  
Novel Approach ◽  
Related Transcription Factor ◽  
Diphenyl Tetrazolium Bromide

Carbon enriched bioceramic (C-Bio) scaffolds have recently shown exceptional results in terms of their biological and mechanical properties. The present study aims at assessing the ability of the C-Bio scaffolds to affect the commitment of canine adipose-derived mesenchymal stem cells (cAD-MSCs) and investigating the influence of carbon on cell proliferation and osteogenic differentiation of cAD-MSCs in vitro. The commitment of cAD-MSCs to an osteoblastic phenotype has been evaluated by expression of several osteogenic markers using real-time PCR. Biocompatibility analyses through 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lactate dehydrogenase (LDH) activity, hemolysis assay, and Ames test demonstrated excellent biocompatibility of both materials. A significant increase in the extracellular alkaline phosphatase (ALP) activity and expression of runt-related transcription factor (RUNX), ALP, osterix (OSX), and receptor activator of nuclear factor kappa-Β ligand (RANKL) genes was observed in C-Bio scaffolds compared to those without carbon (Bio). Scanning electron microscopy (SEM) demonstrated excellent cell attachment on both material surfaces; however, the cellular layer on C-Bio fibers exhibited an apparent secretome activity. Based on our findings, graphene can improve cell adhesion, growth, and osteogenic differentiation of cAD-MSCs in vitro. This study proposed carbon as an additive for a novel three-dimensional (3D)-printable biocompatible scaffold which could become the key structural material for bone tissue reconstruction.

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