Three-Dimensional Hierarchical Composite Scaffolds Consisting of Polycaprolactone, β-Tricalcium Phosphate, and Collagen Nanofibers: Fabrication, Physical Properties, and In Vitro Cell Activity for Bone Tissue Regeneration

2011 ◽  
Vol 12 (2) ◽  
pp. 502-510 ◽  
Author(s):  
MyungGu Yeo ◽  
Hyeongjin Lee ◽  
GeunHyung Kim
Keyword(s):  
Physical Properties ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Cell Activity ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
Composite Scaffolds

scholarly journals Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 61 ◽  
Author(s):  
Yannan Liu ◽  
Juan Gu ◽  
Daidi Fan
Keyword(s):  
Mechanical Properties ◽  
Enzymatic Hydrolysis ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Repair ◽  
Three Dimensional ◽  
High Strength ◽  
Biological Properties ◽  
Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

In vitro evaluation of simvastatin acid (SVA) coated beta-tricalcium phosphate (β-TCP) particle on bone tissue regeneration

2012 ◽  
Vol 20 (7) ◽  
pp. 754-761
Author(s):  
Dae Hyeok Yang ◽  
Min Soo Bae ◽  
Lingjuan Qiao ◽  
Dong Nyoung Heo ◽  
Jung Bok Lee ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Bone Tissue Regeneration ◽  
In Vitro Evaluation ◽  
Beta Tricalcium Phosphate ◽  
Simvastatin Acid

scholarly journals Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Acta Naturae ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 96-101 ◽  
Author(s):  
M. M. Moisenovich ◽  
A. Yu. Arkhipova ◽  
A. A. Orlova ◽  
M. S. Drutskaya ◽  
S. V. Volkova ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Experimental Models ◽  
Bone Tissue Regeneration ◽  
Artificial Organ ◽  
Composite Scaffolds ◽  
Complex Processes ◽  
Healing Bone

Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.

scholarly journals Effect of Morphological Characteristics and Biomineralization of 3D-Printed Gelatin/Hyaluronic Acid/Hydroxyapatite Composite Scaffolds on Bone Tissue Regeneration

2021 ◽  
Vol 22 (13) ◽  
pp. 6794
Author(s):  
Jae-Woo Kim ◽  
Yoon-Soo Han ◽  
Hyun-Mee Lee ◽  
Jin-Kyung Kim ◽  
Young-Jin Kim
Keyword(s):  
Hyaluronic Acid ◽  
Bone Tissue ◽  
Double Layer ◽  
Tissue Regeneration ◽  
Great Influence ◽  
Morphological Characteristics ◽  
Bone Tissue Regeneration ◽  
Hierarchical Architecture ◽  
Uniform Structure ◽  
Composite Scaffolds

The use of porous three-dimensional (3D) composite scaffolds has attracted great attention in bone tissue engineering applications because they closely simulate the major features of the natural extracellular matrix (ECM) of bone. This study aimed to prepare biomimetic composite scaffolds via a simple 3D printing of gelatin/hyaluronic acid (HA)/hydroxyapatite (HAp) and subsequent biomineralization for improved bone tissue regeneration. The resulting scaffolds exhibited uniform structure and homogeneous pore distribution. In addition, the microstructures of the composite scaffolds showed an ECM-mimetic structure with a wrinkled internal surface and a porous hierarchical architecture. The results of bioactivity assays proved that the morphological characteristics and biomineralization of the composite scaffolds influenced cell proliferation and osteogenic differentiation. In particular, the biomineralized gelatin/HA/HAp composite scaffolds with double-layer staggered orthogonal (GEHA20-ZZS) and double-layer alternative structure (GEHA20-45S) showed higher bioactivity than other scaffolds. According to these results, biomineralization has a great influence on the biological activity of cells. Hence, the biomineralized composite scaffolds can be used as new bone scaffolds in bone regeneration.

Engineering 3D printed bioactive composite scaffolds based on the combination of aliphatic polyester and calcium phosphates for bone tissue regeneration

2021 ◽  
Vol 122 ◽  
pp. 111928
Author(s):  
Eduardo H. Backes ◽  
Emanuel M. Fernandes ◽  
Gabriela S. Diogo ◽  
Catarina F. Marques ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Calcium Phosphates ◽  
Bone Tissue Regeneration ◽  
Aliphatic Polyester ◽  
Composite Scaffolds ◽  
3D Printed
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