Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

Lab on a Chip ◽  
2009 ◽  
Vol 9 (21) ◽  
pp. 3110 ◽  
Author(s):  
Sally A. Peyman ◽  
Alexander Iles ◽  
Nicole Pamme
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
Solid Supports

scholarly journals Continuous-Flow Separation of Magnetic Particles from Biofluids: How Does the Microdevice Geometry Determine the Separation Performance?

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3030 ◽  
Author(s):  
Cristina González Fernández ◽  
Jenifer Gómez Pastora ◽  
Arantza Basauri ◽  
Marcos Fallanza ◽  
Eugenio Bringas ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Rational Design ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Permanent Magnets ◽  
System Throughput ◽  
Separation Performance ◽  
High Particle ◽  
Section Shape ◽  
Geometrical Features

The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture; however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation.

Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles

2010 ◽  
Vol 25 (9) ◽  
pp. 2172-2176 ◽  
Author(s):  
Martin Vojtíšek ◽  
Alexander Iles ◽  
Nicole Pamme
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
Dna Hybridisation ◽  
On Chip

scholarly journals Cytochrome c adsorption in a continuous flow system by using Cu(II)-chelated magnetic affinity particles

2021 ◽  
Vol 20 (1) ◽  
pp. 159-163
Author(s):  
Recep AKKAYA ◽  
Birnur AKKAYA
Keyword(s):  
Cytochrome C ◽  
Adsorption Capacity ◽  
Continuous Flow ◽  
Magnetic Particles ◽  
Flow System ◽  
Medical Science ◽  
Hill Coefficient ◽  
Continuous Flow System ◽  
Cyt C ◽  
Poly Ethylene

Background and methodology: In the current study magnetic poly(ethylene glycoldimethacrylate-N-methacryloyl-(L)-histidine methyl ester) poly(Egdma–Mah)) was used as ametal chelated affinity particles. Cu2+ ions loaded directly to MAH ligand of magnetic particlesfor the adsorption of cytochrome c (Cyt c) in a continuous flow system. Results: The maximumCyt c adsorption capacity on the magnetic particles and Cu2+-chelated magnetic particles were42 mg/g and 197 mg/g in phosphate buffer (pH 8.0), respectively. Cu2+ loading increased theCyt c adsorption capacity, significantly. Cyt c adsorption capacity decreased with increasedtemperature and with increasing magnetic field. According to reusability studies Cyt c moleculescould be reversibly adsorbed and desorbed five times. The binding isotherm was determined byscatchard analysis followed by application of Hill equation to the data obtained, then bindingconstant and nH Hill coefficient were calculated. Bangladesh Journal of Medical Science Vol.20(1) 2021 p.159-163

scholarly journals Ceramic-Based Microfluidic Device for Separation of Magnetic Particles in Continuous Flow

2014 ◽  
Vol 26 (6) ◽  
pp. 1571-1574
Author(s):  
Ji-Yun Seon ◽  
Young Joon Yoon ◽  
Kwang Yeon Cho ◽  
Chang-Yeol Kim ◽  
Jong-Hee Kim ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Magnetic Particles

scholarly journals On-Chip Determination of C-Reactive Protein Using Magnetic Particles in Continuous Flow

2014 ◽  
Vol 86 (21) ◽  
pp. 10552-10559 ◽  
Author(s):  
Chayakom Phurimsak ◽  
Mark D. Tarn ◽  
Sally A. Peyman ◽  
John Greenman ◽  
Nicole Pamme
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
On Chip

A magnetic super lattice

1987 ◽  
Vol 45 ◽  
pp. 360-361 ◽  
Author(s):  
M.D. Bentzon ◽  
J. v. Wonterghem ◽  
A. Thölén
Keyword(s):  
Thermal Decomposition ◽  
Oleic Acid ◽  
Magnetic Fluid ◽  
Magnetic Particles ◽  
Carbon Film ◽  
Thick Layer ◽  
Amorphous Iron ◽  
Super Lattice ◽  
Carrier Liquid ◽  
Median Diameter

We report on the oxidation of a magnetic fluid. The oxidation results in magnetic super lattice crystals. The “atoms” are hematite (α-Fe2O3) particles with a diameter ø = 6.9 nm and they are covered with a 1-2 nm thick layer of surfactant molecules.Magnetic fluids are homogeneous suspensions of small magnetic particles in a carrier liquid. To prevent agglomeration, the particles are coated with surfactant molecules. The magnetic fluid studied in this work was produced by thermal decomposition of Fe(CO)5 in Declin (carrier liquid) in the presence of oleic acid (surfactant). The magnetic particles consist of an amorphous iron-carbon alloy. For TEM investigation a droplet of the fluid was added to benzine and a carbon film on a copper net was immersed. When exposed to air the sample starts burning. The oxidation and electron irradiation transform the magnetic particles into hematite (α-Fe2O3) particles with a median diameter ø = 6.9 nm.

Sign in / Sign up

Export Citation Format

Share Document