On-Chip Free-Flow Magnetophoresis:  Continuous Flow Separation of Magnetic Particles and Agglomerates

2004 ◽  
Vol 76 (24) ◽  
pp. 7250-7256 ◽  
Author(s):  
Nicole Pamme ◽  
Andreas Manz
Keyword(s):  
Flow Separation ◽  
Continuous Flow ◽  
Magnetic Particles ◽  
Free Flow ◽  
On Chip

Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles

2010 ◽  
Vol 25 (9) ◽  
pp. 2172-2176 ◽  
Author(s):  
Martin Vojtíšek ◽  
Alexander Iles ◽  
Nicole Pamme
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
Dna Hybridisation ◽  
On Chip

scholarly journals On-Chip Determination of C-Reactive Protein Using Magnetic Particles in Continuous Flow

2014 ◽  
Vol 86 (21) ◽  
pp. 10552-10559 ◽  
Author(s):  
Chayakom Phurimsak ◽  
Mark D. Tarn ◽  
Sally A. Peyman ◽  
John Greenman ◽  
Nicole Pamme
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
On Chip

On-chip tryptic digest with direct coupling to ESI-MS using magnetic particles

2008 ◽  
Vol 29 (24) ◽  
pp. 4944-4947 ◽  
Author(s):  
Anne Le Nel ◽  
Jana Krenkova ◽  
Karel Kleparnik ◽  
Claire Smadja ◽  
Myriam Taverna ◽  
...  
Keyword(s):  
Magnetic Particles ◽  
Direct Coupling ◽  
Esi Ms ◽  
Tryptic Digest ◽  
On Chip

scholarly journals Non-Aqueous Continuous-Flow Electrophoresis (NACFE): Separation Complement for Continuous-Flow Organic Synthesis

2019 ◽  
Author(s):  
Nikita A. Ivanov ◽  
Yimo Liu ◽  
Sven Kochmann ◽  
Sergey N. Krylov
Keyword(s):  
Steady State ◽  
Organic Synthesis ◽  
Flow Separation ◽  
Continuous Flow ◽  
Organic Molecules ◽  
Water Electrolysis ◽  
Organic Salt ◽  
Aqueous Electrolytes ◽  
Differential Distribution ◽  
Steady State Operation

<div>Continuous-flow organic synthesis naturally requires continuous-flow separation of reaction components. The most common continuous-flow separation approach is liquid-liquid extraction based on differential distribution of molecules between organic and aqueous phases. This approach has limited selectivity; it can hardly separate different hydrophobic organic molecules from each other. Continuous-flow electrophoresis can facilitate much more selective separation in a single phase, but it is currently limited to aqueous electrolytes which are incompatible with many hydrophobic organic molecules. Further, water electrolysis in aqueous electrolytes results in generation of large volumes of gas making steady-state operation a major technical challenge. Here, we introduce non-aqueous continuous-flow electrophoresis (NACFE) in which the electrolyte is a solution of an organic salt in an aprotic organic solvent. We demonstrate that NACFE can maintain stable separation of multiple species during 10 hours. The non-aqueous nature of NACFE and its ability to support steady-state operation make it suitable for its incorporation into continuous-flow organic synthesis.</div>

On-chip separation of magnetic particles with different magnetophoretic mobilities

2007 ◽  
Vol 101 (2) ◽  
pp. 024913 ◽  
Author(s):  
Chengxun Liu ◽  
Liesbet Lagae ◽  
Roel Wirix-Speetjens ◽  
Gustaaf Borghs
Keyword(s):  
Magnetic Particles ◽  
On Chip ◽  
Chip Separation

scholarly journals Continuous-Flow Separation of Magnetic Particles from Biofluids: How Does the Microdevice Geometry Determine the Separation Performance?

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3030 ◽  
Author(s):  
Cristina González Fernández ◽  
Jenifer Gómez Pastora ◽  
Arantza Basauri ◽  
Marcos Fallanza ◽  
Eugenio Bringas ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Rational Design ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Permanent Magnets ◽  
System Throughput ◽  
Separation Performance ◽  
High Particle ◽  
Section Shape ◽  
Geometrical Features

The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture; however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation.

The Design of a Continuous Flow Combinatorial Screening Micro Reactor System with On-Chip Detection

2000 ◽  
pp. 59-62
Author(s):  
Victoria Skelton ◽  
Gillian Greenway ◽  
Stephen Haswell ◽  
Peter Styring ◽  
David Morgan ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Reactor System ◽  
Combinatorial Screening ◽  
On Chip ◽  
Micro Reactor

Continuous Dielectrophoretic Separation of Multiple Particles in a Microfluidic Chip

2008 ◽  
Author(s):  
Christian Davidson ◽  
Junjie Zhu ◽  
Xiangchun Xuan
Keyword(s):  
Particle Size ◽  
Electric Field ◽  
Flow Separation ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Uniform Electric Field ◽  
Dielectrophoretic Force ◽  
Size Dependent ◽  
Multiple Particles ◽  
Dc Dielectrophoresis

We successfully demonstrate that DC dielectrophoresis can be utilized to separate particles of three dissimilar sizes simultaneously in a microfluidic chip. This continuous-flow separation is attributed to the particle size dependent dielectrophoretic force that is generated by the non-uniform electric field around a single insulating hurdle on the channel sidewall.

scholarly journals Microfluidic System for Observation of Bacterial Culture and Effects on Biofilm Formation at Microscale

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 606 ◽  
Author(s):  
Xiao-Yan Zhang ◽  
Kai Sun ◽  
Aliya Abulimiti ◽  
Pian-Pian Xu ◽  
Zhe-Yu Li
Keyword(s):  
Membrane Fouling ◽  
Continuous Flow ◽  
Biofilm Formation ◽  
Natural World ◽  
Microfluidic System ◽  
Fluorescence Labeling ◽  
In Situ Observation ◽  
Time Observation ◽  
On Chip ◽  
Bacteria Culture

Biofilms exist in the natural world and applied to many industries. However, due to the variety of characteristics caused by their complex components, biofilms can also lead to membrane fouling and recurrent infections which pose threats to human health. So, to make the best use of their advantages and avoid their disadvantages, knowing the best time and methods for improving or preventing biofilm formation is important. In situ observation without fluorescence labeling in microscale and according to a time scale is useful to research biofilm and confine its formation. In this study, we developed a microfluidic system for real-time observation of bacteria culture and biofilms development at microscale. We cultured E. coli ATCC 25922 on a chip at continuous flow of the velocity, which could promote bacterial formation. Biofilms formation under the condition of adding amoxicillin at different times is also discussed. In addition, the mixed strains from sludge were also cultured on chip, and possible factors in biofilm formation are discussed. Our results show that a microfluidic device could culture microorganisms in continuous flow and accelerate them to adhere to the surface, thereby promoting biofilm formation. Overall, this platform is a useful tool in research on initial biofilm formation, which can contribute to preventing biofouling and infections.

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