A microfluidic system for performing fast, sequential biochemical procedures on the surface of mobile magnetic particles in continuous flow

2009 ◽  
Vol 45 (3) ◽  
pp. 361-370 ◽  
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
Microfluidic System

scholarly journals Pseudo-Continuous Flow FTIR System for Glucose, Fructose and Sucrose Identification in Mid-IR Range

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 517 ◽  
Author(s):  
Hamza Landari ◽  
Mourad Roudjane ◽  
Younès Messaddeq ◽  
Amine Miled
Keyword(s):  
Absorption Spectrum ◽  
Measurement Error ◽  
Wavelength Range ◽  
Continuous Flow ◽  
Flow System ◽  
Microfluidic System ◽  
Sample Distribution ◽  
Peak Intensity ◽  
Conventional Systems

In this paper, we present a new FTIR-based microfluidic system for Glucose, Fructose and Sucrose detection. The proposed microfluidic system is based on a pseudo-continuous flow coupled to a microscope-FTIR instrument. The detection and characterization of sugar samples were performed by recording their absorption spectrum in the wavelength range 700–1000 cm − 1 of the Mid-IR region. The proposed pseudo-continuous flow system is designed to improve the uniformity of the sample distribution in the analyzed area versus conventional systems. The obtained results for different sugars concentrations, show a very low measurement error of 4.35% in the absorption peak intensity, which is ten times lower than the error obtained using the conventional measurements.

scholarly journals Continuous-Flow Separation of Magnetic Particles from Biofluids: How Does the Microdevice Geometry Determine the Separation Performance?

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3030 ◽  
Author(s):  
Cristina González Fernández ◽  
Jenifer Gómez Pastora ◽  
Arantza Basauri ◽  
Marcos Fallanza ◽  
Eugenio Bringas ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Rational Design ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Permanent Magnets ◽  
System Throughput ◽  
Separation Performance ◽  
High Particle ◽  
Section Shape ◽  
Geometrical Features

The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture; however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation.

scholarly journals Microfluidic System for Observation of Bacterial Culture and Effects on Biofilm Formation at Microscale

Micromachines ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 606 ◽  
Author(s):  
Xiao-Yan Zhang ◽  
Kai Sun ◽  
Aliya Abulimiti ◽  
Pian-Pian Xu ◽  
Zhe-Yu Li
Keyword(s):  
Membrane Fouling ◽  
Continuous Flow ◽  
Biofilm Formation ◽  
Natural World ◽  
Microfluidic System ◽  
Fluorescence Labeling ◽  
In Situ Observation ◽  
Time Observation ◽  
On Chip ◽  
Bacteria Culture

Biofilms exist in the natural world and applied to many industries. However, due to the variety of characteristics caused by their complex components, biofilms can also lead to membrane fouling and recurrent infections which pose threats to human health. So, to make the best use of their advantages and avoid their disadvantages, knowing the best time and methods for improving or preventing biofilm formation is important. In situ observation without fluorescence labeling in microscale and according to a time scale is useful to research biofilm and confine its formation. In this study, we developed a microfluidic system for real-time observation of bacteria culture and biofilms development at microscale. We cultured E. coli ATCC 25922 on a chip at continuous flow of the velocity, which could promote bacterial formation. Biofilms formation under the condition of adding amoxicillin at different times is also discussed. In addition, the mixed strains from sludge were also cultured on chip, and possible factors in biofilm formation are discussed. Our results show that a microfluidic device could culture microorganisms in continuous flow and accelerate them to adhere to the surface, thereby promoting biofilm formation. Overall, this platform is a useful tool in research on initial biofilm formation, which can contribute to preventing biofouling and infections.

Dielectrophoresis-Induced Separation of Metallic and Semiconducting Single-Wall Carbon Nanotubes in a Continuous Flow Microfluidic System

2007 ◽  
Vol 7 (10) ◽  
pp. 3431-3435 ◽  
Author(s):  
Mattias Mattsson ◽  
Andrei Gromov ◽  
Staffan Dittmer ◽  
Emma Eriksson ◽  
Oleg A. Nerushev ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Continuous Flow ◽  
Microfluidic System ◽  
Single Wall Carbon Nanotubes ◽  
Single Wall Carbon

Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

Lab on a Chip ◽  
2009 ◽  
Vol 9 (21) ◽  
pp. 3110 ◽  
Author(s):  
Sally A. Peyman ◽  
Alexander Iles ◽  
Nicole Pamme
Keyword(s):  
Continuous Flow ◽  
Magnetic Particles ◽  
Solid Supports

scholarly journals Magnetic Microreactors with Immobilized Enzymes—From Assemblage to Contemporary Applications

Catalysts ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 282 ◽  
Author(s):  
Elena Gkantzou ◽  
Michaela Patila ◽  
Haralambos Stamatis
Keyword(s):  
Magnetic Particles ◽  
Catalytic Properties ◽  
Immobilized Enzymes ◽  
Microfluidic System ◽  
System Configuration ◽  
Reaction Parameters ◽  
Magnetic Forces ◽  
Research Fields ◽  
Enzymatic Microreactors ◽  
Novel Applications

Microfluidics, as the technology for continuous flow processing in microscale, is being increasingly elaborated on in enzyme biotechnology and biocatalysis. Enzymatic microreactors are a precious tool for the investigation of catalytic properties and optimization of reaction parameters in a thriving and high-yielding way. The utilization of magnetic forces in the overall microfluidic system has reinforced enzymatic processes, paving the way for novel applications in a variety of research fields. In this review, we hold a discussion on how different magnetic particles combined with the appropriate biocatalyst under the proper system configuration may constitute a powerful microsystem and provide a highly explorable scope.

scholarly journals Microfluidic conformal coating of non-spherical magnetic particles

2021 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Navid Hakimi ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Thin Film ◽  
Interfacial Tension ◽  
Magnetic Particles ◽  
Magnetic Field Gradient ◽  
Interfacial Area ◽  
Aqueous Fluid ◽  
Microfluidic System ◽  
Immiscible Fluids ◽  
Spherical Particles ◽  
Conformal Coating

We present the conformal coating of non-spherical magnetic particles in a co-laminar flow microfluidic system. Whereas in the previous reports spherical particles had been coated with thin films that formed spheres around the particles; in this article, we show the coating of non-spherical particles with coating layers that are approximately uniform in thickness. The novelty of our work is that while liquid-liquid interfacial tension tends to minimize the surface area of interfaces—for example, to form spherical droplets that encapsulate spherical particles—in our experiments, the thin film that coats non-spherical particles has a non-minimal interfacial area. We first make bullet-shaped magnetic microparticles using a stop-flow lithography method that was previously demonstrated. We then suspend the bullet-shaped microparticles in an aqueous solution and flow the particle suspension with a co-flow of a non-aqueous mixture. A magnetic field gradient from a permanent magnet pulls the microparticles in the transverse direction to the fluid flow, until the particles reach the interface between the immiscible fluids. We observe that upon crossing the oil-water interface, the microparticles become coated by a thin film of the aqueous fluid. When we increase the two-fluid interfacial tension by reducing surfactant concentration, we observe that the particles become trapped at the interface, and we use this observation to extract an approximate magnetic susceptibility of the manufactured non-spherical microparticles. Finally, using fluorescence imaging, we confirm the uniformity of the thin film coating along the entire curved surface of the bullet-shaped particles. To the best of our knowledge, this is the first demonstration of conformal coating of non-spherical particles using microfluidics.

scholarly journals Dynamic Halbach array magnet integrated microfluidic system for the continuous-flow separation of rare tumor cells

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38496-38504 ◽  
Author(s):  
Mei Xue ◽  
An Xiang ◽  
Yanhai Guo ◽  
Li Wang ◽  
Rou Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Flow Separation ◽  
Whole Blood ◽  
Continuous Flow ◽  
Microfluidic System ◽  
Rare Tumor ◽  
Halbach Array

We develop a dynamic Halbach array magnet integrated microfluidic system for continuous-flow separation of circulating tumor cells from whole blood.

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