Rapid and selective extraction, isolation, preconcentration, and quantitation of small RNAs from cell lysate using on-chip isotachophoresis

Lab on a Chip ◽  
2009 ◽  
Vol 9 (15) ◽  
pp. 2145 ◽  
Author(s):  
Reto B. Schoch ◽  
Mostafa Ronaghi ◽  
Juan G. Santiago
Keyword(s):  
Small Rnas ◽  
Selective Extraction ◽  
Cell Lysate ◽  
On Chip

scholarly journals Label-Free On-Chip Selective Extraction of Cell-Aggregate-Laden Microcapsules from Oil into Aqueous Solution with Optical Sensor and Dielectrophoresis

ACS Sensors ◽  
2018 ◽  
Vol 3 (2) ◽  
pp. 410-417 ◽  
Author(s):  
Mingrui Sun ◽  
Patrick Durkin ◽  
Jianrong Li ◽  
Thomas L. Toth ◽  
Xiaoming He
Keyword(s):  
Aqueous Solution ◽  
Optical Sensor ◽  
Selective Extraction ◽  
Cell Aggregate ◽  
Label Free ◽  
On Chip

scholarly journals Deep Learning‐Enabled Label‐Free On‐Chip Detection and Selective Extraction of Cell Aggregate‐Laden Hydrogel Microcapsules

Small ◽  
2021 ◽  
Vol 17 (24) ◽  
pp. 2102868
Author(s):  
Alisa M. White ◽  
Yuntian Zhang ◽  
James G. Shamul ◽  
Jiangsheng Xu ◽  
Elyahb A. Kwizera ◽  
...  
Keyword(s):  
Deep Learning ◽  
Selective Extraction ◽  
Cell Aggregate ◽  
Label Free ◽  
On Chip

On-chip isotachophoresis separation of functional DNA origami capture nanoarrays from cell lysate

Nano Research ◽  
2013 ◽  
Vol 6 (10) ◽  
pp. 712-719 ◽  
Author(s):  
Qian Mei ◽  
Roger H. Johnson ◽  
Xixi Wei ◽  
Fengyu Su ◽  
Yan Liu ◽  
...  
Keyword(s):  
Dna Origami ◽  
Cell Lysate ◽  
Functional Dna ◽  
On Chip

Isolation of enriched small RNA from cell‐lysate using on‐chip isotachophoresis

2019 ◽  
Vol 40 (23-24) ◽  
pp. 3140-3147
Author(s):  
Ruba Khnouf ◽  
Crystal M. Han ◽  
Sarah A. Munro
Keyword(s):  
Small Rna ◽  
Cell Lysate ◽  
On Chip

Deep Learning‐Enabled Label‐Free On‐Chip Detection and Selective Extraction of Cell Aggregate‐Laden Hydrogel Microcapsules

Small ◽  
2021 ◽  
pp. 2100491
Author(s):  
Alisa M. White ◽  
Yuntian Zhang ◽  
James G. Shamul ◽  
Jiangsheng Xu ◽  
Elyahb A. Kwizera ◽  
...  
Keyword(s):  
Deep Learning ◽  
Selective Extraction ◽  
Cell Aggregate ◽  
Label Free ◽  
On Chip

Gut microbiome a promising target for management of respiratory diseases

2020 ◽  
Vol 477 (14) ◽  
pp. 2679-2696
Author(s):  
Riddhi Trivedi ◽  
Kalyani Barve
Keyword(s):  
Respiratory Diseases ◽  
New Technology ◽  
Bacterial Flora ◽  
Systemic Circulation ◽  
The Body ◽  
Lung Pathology ◽  
Promising Target ◽  
Current Therapies ◽  
Intestinal Microbial Flora ◽  
On Chip

The intestinal microbial flora has risen to be one of the important etiological factors in the development of diseases like colorectal cancer, obesity, diabetes, inflammatory bowel disease, anxiety and Parkinson's. The emergence of the association between bacterial flora and lungs led to the discovery of the gut–lung axis. Dysbiosis of several species of colonic bacteria such as Firmicutes and Bacteroidetes and transfer of these bacteria from gut to lungs via lymphatic and systemic circulation are associated with several respiratory diseases such as lung cancer, asthma, tuberculosis, cystic fibrosis, etc. Current therapies for dysbiosis include use of probiotics, prebiotics and synbiotics to restore the balance between various species of beneficial bacteria. Various approaches like nanotechnology and microencapsulation have been explored to increase the permeability and viability of probiotics in the body. The need of the day is comprehensive study of mechanisms behind dysbiosis, translocation of microbiota from gut to lung through various channels and new technology for evaluating treatment to correct this dysbiosis which in turn can be used to manage various respiratory diseases. Microfluidics and organ on chip model are emerging technologies that can satisfy these needs. This review gives an overview of colonic commensals in lung pathology and novel systems that help in alleviating symptoms of lung diseases. We have also hypothesized new models to help in understanding bacterial pathways involved in the gut–lung axis as well as act as a futuristic approach in finding treatment of respiratory diseases caused by dysbiosis.

A possibility of using nickel concentrate, obtained as a result of hydrometallurgical enrichment of manganese-containing raw materials, at steel alloying

2020 ◽  
Vol 76 (7) ◽  
pp. 709-715
Author(s):  
O. Yu. Kichigina
Keyword(s):  
Raw Materials ◽  
Experimental Studies ◽  
Selective Extraction ◽  
Technological Parameters ◽  
Nickel Iron ◽  
Nickel Recovery ◽  
Nickel Concentrate ◽  
Steel Alloying ◽  
High Degree ◽  
Comprehensive Processing

At production of stainless steel expensive alloying elements, containing nickel, are used. To decrease the steel cost, substitution of nickel during steel alloying process by its oxides is an actual task. Results of analysis of thermodynamic and experimental studies of nickel reducing from its oxide presented, as well as methods of nickel oxide obtaining at manganese bearing complex raw materials enrichment and practice of its application during steel alloying. Technology of comprehensive processing of complex manganese-containing raw materials considered, including leaching and selective extraction out of the solution valuable components: manganese, nickel, iron, cobalt and copper. Based on theoretical and experiment studies, a possibility of substitution of metal nickel by concentrates, obtained as a result of hydrometallurgical enrichment, was confirmed. Optimal technological parameters, ensuring high degree of nickel recovery out of the initial raw materials were determined. It was established, that for direct steel alloying it is reasonable to add into the charge pellets, consisting of nickel concentrate and coke fines, that enables to reach the through nickel recovery at a level of 90%. The proposed method of alloying steel by nickel gives a possibility to decrease considerably steel cost at the expense of application of nickel concentrate, obtained out of tails of hydrometallurgical enrichment of manganese-bearing raw materials, which is much cheaper comparing with the metal nickel.

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