Self-referencing a single waveguide grating sensor in a micron-sized deep flow chamber for label-free biomolecular binding assays

Lab on a Chip ◽  
2005 ◽  
Vol 5 (9) ◽  
pp. 959 ◽  
Author(s):  
Po Ki Yuen ◽  
Norman H. Fontaine ◽  
Mark A. Quesada ◽  
Prantik Mazumder ◽  
Richard Bergman ◽  
...  
Keyword(s):  
Flow Chamber ◽  
Label Free ◽  
Binding Assays ◽  
Waveguide Grating ◽  
Biomolecular Binding

scholarly journals How to copy and paste DNA microarrays

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stefan D. Krämer ◽  
Johannes Wöhrle ◽  
Philipp A. Meyer ◽  
Gerald A. Urban ◽  
Günter Roth
Keyword(s):  
Real Time ◽  
Dna Microarray ◽  
Dna Microarrays ◽  
Label Free ◽  
Binding Assays ◽  
Single Stranded Dna ◽  
Whole Process ◽  
Double Stranded Dna ◽  
Cost Efficient ◽  
Copy And Paste

Abstract Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.

scholarly journals Combining Label‐free and Flourometric Functional Assays with Biophysical Binding Assays in Drug Discovery: A Comparison of Positives from an HTS Campaign

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Matthew Wayne Olson ◽  
Jukka Kervinen ◽  
Carsten Schubert ◽  
Jennifer Kirkpatrick ◽  
James Kranz ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Label Free ◽  
Binding Assays ◽  
Functional Assays

scholarly journals Label-Free Plasmonic Detection of Biomolecular Binding by a Single Gold Nanorod

2008 ◽  
Vol 80 (4) ◽  
pp. 984-989 ◽  
Author(s):  
Greg J. Nusz ◽  
Stella M. Marinakos ◽  
Adam C. Curry ◽  
Andreas Dahlin ◽  
Fredrik Höök ◽  
...  
Keyword(s):  
Gold Nanorod ◽  
Label Free ◽  
Biomolecular Binding

scholarly journals Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array

2002 ◽  
Vol 99 (15) ◽  
pp. 9783-9788 ◽  
Author(s):  
R. McKendry ◽  
J. Zhang ◽  
Y. Arntz ◽  
T. Strunz ◽  
M. Hegner ◽  
...  
Keyword(s):  
Dna Binding ◽  
Label Free ◽  
Binding Assays ◽  
Cantilever Array

scholarly journals Detection of Myoglobin with an Open-Cavity-Based Label-Free Photonic Crystal Biosensor

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Bailin Zhang ◽  
Juan Manuel Tamez-Vela ◽  
Steven Solis ◽  
Gilbert Bustamante ◽  
Ralph Peterson ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Point Of Care ◽  
Microfluidic System ◽  
Cardiac Biomarkers ◽  
Detection Sensitivity ◽  
Label Free ◽  
Binding Assays ◽  
Biochemical Modification ◽  
Label Free Detection ◽  
Biomolecular Assays

The label-free detection of one of the cardiac biomarkers, myoglobin, using a photonic-crystal-based biosensor in a total-internal-reflection configuration (PC-TIR) is presented in this paper. The PC-TIR sensor possesses a unique open optical microcavity that allows for several key advantages in biomolecular assays. In contrast to a conventional closed microcavity, the open configuration allows easy functionalization of the sensing surface for rapid biomolecular binding assays. Moreover, the properties of PC structures make it easy to be designed and engineered for operating at any optical wavelength. Through fine design of the photonic crystal structure, biochemical modification of the sensor surface, and integration with a microfluidic system, we have demonstrated that the detection sensitivity of the sensor for myoglobin has reached the clinically significant concentration range, enabling potential usage of this biosensor for diagnosis of acute myocardial infarction. The real-time response of the sensor to the myoglobin binding may potentially provide point-of-care monitoring of patients and treatment effects.

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