scholarly journals Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Norbert Orgovan ◽  
Beatrix Peter ◽  
Szilvia Bősze ◽  
Jeremy J. Ramsden ◽  
Bálint Szabó ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Cell ◽  
High Throughput ◽  
Surface Density ◽  
Label Free ◽  
Waveguide Grating ◽  
Cancer Cell Adhesion ◽  
Resonant Waveguide Grating ◽  
Integrin Ligand

scholarly journals Glycocalyx regulates the strength and kinetics of cancer cell adhesion revealed by biophysical models based on high resolution label-free optical data

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicolett Kanyo ◽  
Kinga Dora Kovacs ◽  
Andras Saftics ◽  
Inna Szekacs ◽  
Beatrix Peter ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
High Resolution ◽  
Cancer Cell ◽  
Kinetic Data ◽  
Dermatan Sulfate ◽  
Cellular Adhesion ◽  
Label Free ◽  
Cancer Cell Adhesion ◽  
Kinetics Of ◽  
Adhesion Process

AbstractThe glycocalyx is thought to perform a potent, but not yet defined function in cellular adhesion and signaling. Since 95% of cancer cells have altered glycocalyx structure, this role can be especially important in cancer development and metastasis. The glycocalyx layer of cancer cells directly influences cancer progression, involving the complicated kinetic process of cellular adhesion at various levels. In the present work, we investigated the effect of enzymatic digestion of specific glycocalyx components on cancer cell adhesion to RGD (arginine–glycine–aspartic acid) peptide motif displaying surfaces. High resolution kinetic data of cell adhesion was recorded by the surface sensitive label-free resonant waveguide grating (RWG) biosensor, supported by fluorescent staining of the cells and cell surface charge measurements. We found that intense removal of chondroitin sulfate (CS) and dermatan sulfate chains by chondroitinase ABC reduced the speed and decreased the strength of adhesion of HeLa cells. In contrast, mild digestion of glycocalyx resulted in faster and stronger adhesion. Control experiments on a healthy and another cancer cell line were also conducted, and the discrepancies were analysed. We developed a biophysical model which was fitted to the kinetic data of HeLa cells. Our analysis suggests that the rate of integrin receptor transport to the adhesion zone and integrin-RGD binding is strongly influenced by the presence of glycocalyx components, but the integrin-RGD dissociation is not. Moreover, based on the kinetic data we calculated the dependence of the dissociation constant of integrin-RGD binding on the enzyme concentration. We also determined the dissociation constant using a 2D receptor binding model based on saturation level static data recorded at surfaces with tuned RGD densities. We analyzed the discrepancies of the kinetic and static dissociation constants, further illuminating the role of cancer cell glycocalyx during the adhesion process. Altogether, our experimental results and modelling demonstrated that the chondroitin sulfate and dermatan sulfate chains of glycocalyx have an important regulatory function during the cellular adhesion process, mainly controlling the kinetics of integrin transport and integrin assembly into mature adhesion sites. Our results potentially open the way for novel type of cancer treatments affecting these regulatory mechanisms of cellular glycocalyx.

scholarly journals Label-Free High-Throughput Functional Lytic Assays

2006 ◽  
Vol 12 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Shawn M. O'Malley ◽  
Xinying Xie ◽  
Anthony G. Frutos
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Label Free ◽  
Waveguide Grating ◽  
Protein Substrates ◽  
Enzyme Substrate ◽  
Multiple Protein ◽  
Ic50 Values ◽  
Amine Groups ◽  
Resonant Waveguide Grating

Refractive index-sensitive resonant waveguide grating biosensors are used to assay the label-free enzymatic degradation of biomolecules. These assays provide a robust means of screening for functional lytic modulators. The biomolecular substrates in this study were covalently immobilized through amine groups. Using the Corning® Epic™ System, the digestion signatures for multiple protein substrates on the biosensors are measured. Label-free digestion profiles for these proteins were substrate specific. Similarly, the authors find that the label-free digestion is protease specific. Enzyme-substrate pairs were used to evaluate high- throughput biosensors as tools for screening functional modulators. The lytic inhibitor properties for several proteases and dextranase are determined. The authors find that the IC50 values for the protease inhibitors agree with the reported values for several known inhibitors. The Ź values, using biosensor-based functional lytic screens, were routinely greater than 0.5, making this label-free application feasible for high-throughput screening.

scholarly journals Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion

2013 ◽  
Vol 188 ◽  
pp. 1064-1072 ◽  
Author(s):  
Natalya Zaytseva ◽  
Jeffery G. Lynn ◽  
Qi Wu ◽  
Deepti J. Mudaliar ◽  
Haiyan Sun ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Fluorescence Detection ◽  
Label Free ◽  
Waveguide Grating ◽  
Resonant Waveguide Grating

scholarly journals Lipogenic signalling modulates prostate cancer cell adhesion and migration via modification of Rho GTPases

Oncogene ◽  
2020 ◽  
Vol 39 (18) ◽  
pp. 3666-3679 ◽  
Author(s):  
Mario De Piano ◽  
Valeria Manuelli ◽  
Giorgia Zadra ◽  
Jonathan Otte ◽  
Per-Henrik D. Edqvist ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Rho Gtpases ◽  
Prostate Cancer Cell ◽  
Cancer Cell Adhesion ◽  
Cell Adhesion And Migration ◽  
And Migration

scholarly journals CXCL2-CXCR2 axis mediates αV integrin-dependent peritoneal metastasis of colon cancer cells

2021 ◽  
Author(s):  
Mattias Lepsenyi ◽  
Nader Algethami ◽  
Amr A. Al-Haidari ◽  
Anwar Algaber ◽  
Ingvar Syk ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Adhesion ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Colon Cancer Cell ◽  
Colon Cancer Cells ◽  
Cancer Cell Adhesion ◽  
And Migration ◽  
Cxcr2 Antagonist

AbstractPeritoneal metastasis is an insidious aspect of colorectal cancer. The aim of the present study was to define mechanisms regulating colon cancer cell adhesion and spread to peritoneal wounds after abdominal surgery. Mice was laparotomized and injected intraperitoneally with CT-26 colon carcinoma cells and metastatic noduli in the peritoneal cavity was quantified after treatment with a CXCR2 antagonist or integrin-αV-antibody. CT-26 cells expressed cell surface chemokine receptors CXCR2, CXCR3, CXCR4 and CXCR5. Stimulation with the CXCR2 ligand, CXCL2, dose-dependently increased proliferation and migration of CT-26 cells in vitro. The CXCR2 antagonist, SB225002, dose-dependently decreased CXCL2-induced proliferation and migration of colon cancer cells in vitro. Intraperitoneal administration of CT-26 colon cancer cells resulted in wide-spread growth of metastatic nodules at the peritoneal surface of laparotomized animals. Laparotomy increased gene expression of CXCL2 at the incisional line. Pretreatment with CXCR2 antagonist reduced metastatic nodules by 70%. Moreover, stimulation with CXCL2 increased CT-26 cell adhesion to extracellular matrix (ECM) proteins in a CXCR2-dependent manner. CT-26 cells expressed the αV, β1 and β3 integrin subunits and immunoneutralization of αV abolished CXCL2-triggered adhesion of CT-26 to vitronectin, fibronectin and fibrinogen. Finally, inhibition of the αV integrin significantly attenuated the number of carcinomatosis nodules by 69% in laparotomized mice. These results were validated by use of the human colon cancer cell line HT-29 in vitro. Our data show that colon cancer cell adhesion and growth on peritoneal wound sites is mediated by a CXCL2-CXCR2 signaling axis and αV integrin-dependent adhesion to ECM proteins.

Inhibition of Cancer Cell Adhesion, Migration and Proliferation by a Bispecific Antibody That Targets Two Distinct Epitopes on αv Integrins

2021 ◽  
pp. 167090
Author(s):  
Eugenio Gallo ◽  
Abdellali Kelil ◽  
Michael Haughey ◽  
Mariana Cazares-Olivera ◽  
Bradley P. Yates ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Cell ◽  
Bispecific Antibody ◽  
Cancer Cell Adhesion

scholarly journals Senescent Peritoneal Mesothelial Cells Promote Ovarian Cancer Cell Adhesion

2009 ◽  
Vol 174 (4) ◽  
pp. 1230-1240 ◽  
Author(s):  
Krzysztof Ksiazek ◽  
Justyna Mikula-Pietrasik ◽  
Katarzyna Korybalska ◽  
Grzegorz Dworacki ◽  
Achim Jörres ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Cancer Cell Adhesion

Prostate Cancer Cell Adhesion to Bone Marrow Endothelium

2004 ◽  
Vol 64 (6) ◽  
pp. 2083-2089 ◽  
Author(s):  
Victor I. Romanov ◽  
Terry Whyard ◽  
Howard L. Adler ◽  
Wayne C. Waltzer ◽  
Stanley Zucker
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Adhesion
Sign in / Sign up

Export Citation Format

Share Document