Label-free molecular binding assays using holographic video microscopy

2011 ◽  
Author(s):  
Fook Chiong Cheong ◽  
David G. Grier
Keyword(s):  
Video Microscopy ◽  
Label Free ◽  
Binding Assays ◽  
Molecular Binding ◽  
Holographic Video Microscopy

Self-referencing a single waveguide grating sensor in a micron-sized deep flow chamber for label-free biomolecular binding assays

Lab on a Chip ◽  
2005 ◽  
Vol 5 (9) ◽  
pp. 959 ◽  
Author(s):  
Po Ki Yuen ◽  
Norman H. Fontaine ◽  
Mark A. Quesada ◽  
Prantik Mazumder ◽  
Richard Bergman ◽  
...  
Keyword(s):  
Flow Chamber ◽  
Label Free ◽  
Binding Assays ◽  
Waveguide Grating ◽  
Biomolecular Binding

scholarly journals How to copy and paste DNA microarrays

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stefan D. Krämer ◽  
Johannes Wöhrle ◽  
Philipp A. Meyer ◽  
Gerald A. Urban ◽  
Günter Roth
Keyword(s):  
Real Time ◽  
Dna Microarray ◽  
Dna Microarrays ◽  
Label Free ◽  
Binding Assays ◽  
Single Stranded Dna ◽  
Whole Process ◽  
Double Stranded Dna ◽  
Cost Efficient ◽  
Copy And Paste

Abstract Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.

scholarly journals Holographic molecular binding assays

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yvonne Zagzag ◽  
M. Francesca Soddu ◽  
Andrew D. Hollingsworth ◽  
David G. Grier
Keyword(s):  
Binding Assays ◽  
Molecular Binding

scholarly journals Detection of molecular binding via charge-induced mechanical response of optical fibers

2014 ◽  
Vol 5 (11) ◽  
pp. 4375-4381 ◽  
Author(s):  
Yan Guan ◽  
Xiaonan Shan ◽  
Shaopeng Wang ◽  
Peiming Zhang ◽  
Nongjian Tao
Keyword(s):  
Optical Fibers ◽  
Molecular Interactions ◽  
Mechanical Response ◽  
Optical Detection ◽  
Detection Technique ◽  
Label Free ◽  
Molecular Binding ◽  
A Charge

We report a charge sensitive optical detection technique for the label-free study of molecular interactions.

Real-time label-free detection of dividing cells by means of lensfree video-microscopy

2014 ◽  
Vol 19 (03) ◽  
pp. 1 ◽  
Author(s):  
Srikanth V. Kesavan ◽  
Fabrice P. Navarro Y Garcia ◽  
Mathilde Menneteau ◽  
Frédérique Mittler ◽  
Brigitte David-Watine ◽  
...  
Keyword(s):  
Real Time ◽  
Video Microscopy ◽  
Label Free ◽  
Label Free Detection ◽  
Dividing Cells

scholarly journals Profiling Established Cell Lines as a Means to Screening Diversity

1998 ◽  
Vol 3 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Evelyn Good ◽  
Scott Perschke ◽  
Rani Lopez ◽  
Sonia Chang ◽  
April Kinsler ◽  
...  
Keyword(s):  
Cell Lines ◽  
Specific Binding ◽  
Target Material ◽  
Type I ◽  
Binding Assays ◽  
Molecular Binding ◽  
Established Cell Lines ◽  
Established Cell ◽  
Adenocarcinoma Of The Colon ◽  
Cell Profile

Cell lines provide a readily available source of target material for functional and molecular binding screens in drug discovery. The Cell PROFILE® program at NovaScreen® represents an effort to identify receptors and enzymes expressed in established cell lines that are relevant to important drug screening endeavors. In this report, we present data on a selected number of receptors and enzymes for four cell lines studied in this survey. The objective of this survey was not to compare one cell line with another, but to illustrate the diversity of pharmacologic targets and the untapped potential of databases for readily obtainable cell lines. The following cell lines, which are all derived from human tumors, were included in this study (with some relevant pharmacologic/pathologic targets): HT-29, derived from an adenocarcinoma of the colon (colorectal cancer); SK-N-MC, derived from a neuroepithelioma (NPY receptors, apoptosis, HIV type I infection); H-4, derived from a neuroglioma (Alzheimer's disease); and LNCaP, derived from a prostate carcinoma (androgen receptor, prostate cancer). Specific to this survey were receptor-binding assays for androgens, corticotropin-releasing factor, endothelin, GABA, NMDA, somatostatin, and alpha and beta adrenergic ligands, as well as binding sites for ion channels. A comparison of specific binding of these various sites between target tissues routinely used in our assays and established cell lines reveals a diversity of receptors heretofore not reported for the latter and represents a potential database for screening and pharmacologic research.

scholarly journals Combining Label‐free and Flourometric Functional Assays with Biophysical Binding Assays in Drug Discovery: A Comparison of Positives from an HTS Campaign

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Matthew Wayne Olson ◽  
Jukka Kervinen ◽  
Carsten Schubert ◽  
Jennifer Kirkpatrick ◽  
James Kranz ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Label Free ◽  
Binding Assays ◽  
Functional Assays

scholarly journals Flow visualization and flow cytometry with holographic video microscopy

2009 ◽  
Vol 17 (15) ◽  
pp. 13071 ◽  
Author(s):  
Fook Chiong Cheong ◽  
Bo Sun Rémi Dreyfus ◽  
Jesse Amato-Grill ◽  
Ke Xiao ◽  
Lisa Dixon ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Flow Visualization ◽  
Video Microscopy ◽  
Holographic Video Microscopy
Sign in / Sign up

Export Citation Format

Share Document