A dual-purpose basal medium for the microbiological assay of nicotinic acid (niacin) and pantothenic acid with Lactobacillus plantarum

The Analyst ◽  
1972 ◽  
Vol 97 (1153) ◽  
pp. 300
Author(s):  
E. C. Barton-Wright
Keyword(s):  
Nicotinic Acid ◽  
Lactobacillus Plantarum ◽  
Pantothenic Acid ◽  
Basal Medium ◽  
Microbiological Assay ◽  
Dual Purpose

Radioimmunoassay for pantothenic acid in blood and other tissues.

1979 ◽  
Vol 25 (1) ◽  
pp. 108-111 ◽  
Author(s):  
B W Wyse ◽  
C Wittwer ◽  
R G Hansen
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Lactobacillus Plantarum ◽  
Bovine Serum ◽  
Liquid Scintillation ◽  
Scintillation Counter ◽  
Pantothenic Acid ◽  
Biological Tissues ◽  
Binding Studies ◽  
Tissue Extracts

Abstract We describe a radioimmunoassay for pantothenic acid in biological tissues. D-Pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. For the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled pantothentic acid. The antibodies were precipitated and dissolved, and the radioactivity of the solution was measured in a liquid scintillation counter. Between 5 and 125 ng of pantothenic acid can be detected in 75 muL of tissue extract. Validation included recovery and precision studies, parallelism with tissue extracts, and competitive binding studies. Results of the radioimmunoassay and those of microbiological assay with use of Lactobacillus plantarum correlated well (r = 0.80).

scholarly journals Adaptive laboratory evolution and reverse engineering of single-vitamin prototrophies in Saccharomyces cerevisiae

2020 ◽  
Author(s):  
Thomas Perli ◽  
Dewi P.I. Moonen ◽  
Marcel van den Broek ◽  
Jack T. Pronk ◽  
Jean-Marc Daran
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Nicotinic Acid ◽  
Specific Growth ◽  
Pantothenic Acid ◽  
B Vitamins ◽  
Fast Growth ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
B Vitamin

AbstractQuantitative physiological studies on Saccharomyces cerevisiae commonly use synthetic media (SM) that contain a set of water-soluble growth factors that, based on their roles in human nutrition, are referred to as B-vitamins. Previous work demonstrated that, in S. cerevisiae CEN.PK113-7D, requirements for biotin could be eliminated by laboratory evolution. In the present study, this laboratory strain was shown to exhibit suboptimal specific growth rates when either inositol, nicotinic acid, pyridoxine, pantothenic acid, para-aminobenzoic acid (pABA) or thiamine were omitted from SM. Subsequently, this strain was evolved in parallel serial-transfer experiments for fast aerobic growth on glucose in the absence of individual B-vitamins. In all evolution lines, specific growth rates reached at least 90 % of the growth rate observed in SM supplemented with a complete B-vitamin mixture. Fast growth was already observed after a few transfers on SM without myo-inositol, nicotinic acid or pABA. Reaching similar results in SM lacking thiamine, pyridoxine or pantothenate required over 300 generations of selective growth. The genomes of evolved single-colony isolates were re-sequenced and, for each B-vitamin, a subset of non-synonymous mutations associated with fast vitamin-independent growth were selected. These mutations were introduced in a non-evolved reference strain using CRISPR/Cas9-based genome editing. For each B-vitamin, introduction of a small number of mutations sufficed to achieve substantially a increased specific growth rate in non-supplemented SM that represented at least 87% of the specific growth rate observed in fully supplemented complete SM.ImportanceMany strains of Saccharomyces cerevisiae, a popular platform organism in industrial biotechnology, carry the genetic information required for synthesis of biotin, thiamine, pyridoxine, para-aminobenzoic acid, pantothenic acid, nicotinic acid and inositol. However, omission of these B-vitamins typically leads to suboptimal growth. This study demonstrates that, for each individual B-vitamin, it is possible to achieve fast vitamin-independent growth by adaptive laboratory evolution (ALE). Identification of mutations responsible for these fast-growing phenotype by whole-genome sequencing and reverse engineering showed that, for each compound, a small number of mutations sufficed to achieve fast growth in its absence. These results form an important first step towards development of S. cerevisiae strains that exhibit fast growth on cheap, fully mineral media that only require complementation with a carbon source, thereby reducing costs, complexity and contamination risks in industrial yeast fermentation processes.

B-COMPLEX VITAMIN SUPPLEMENTATION OF PIG RATIONS BASED ON BARLEY GROWN ON GREY WOODED SOILS

1962 ◽  
Vol 42 (2) ◽  
pp. 183-190 ◽  
Author(s):  
B. D. Owen ◽  
A. Reddon ◽  
F. Whiting
Keyword(s):  
Nicotinic Acid ◽  
Post Partum ◽  
Pantothenic Acid ◽  
Vitamin Supplementation ◽  
Vitamin B ◽  
Growth Responses ◽  
Growing Period ◽  
Peace River ◽  
River Region ◽  
B Vitamin

Ninety-two sows and two hundred and sixteen pigs were employed in four gestation-lactation and three growing-finishing experiments to determine the adequacy of barley grown on the Grey Wooded soils of the Peace River region in northwestern Canada, as a source of riboflavin, pantothenic and nicotinic acid in swine rations. Vitamin B12 was assessed as a dietary essential in the all-plant rations. In the gestation-lactation experiments sow gains during gestation were greater in the B-vitamin supplemented lot and a trend toward heavier weaning weights of pigs was evident. Vitamin supplementation increased the level of vitamin B12 in sow’s milk collected 1 week post-partum and of both riboflavin and pantothenic acid collected 1 week and 6 weeks post-partum. Nicotinic acid content of sows’ milk was unaffected by supplementation. There was a highly significant decrease in riboflavin level in both control and supplemented lots between 1 and 6 weeks following parturition. The decrease was greatest in the unsupplemented lot.B-vitamin supplementation did not affect growing period gains or pig carcass quality in the growing-finishing experiments. However, certain significant growth responses were noted in the finishing period.The data indicate that the basal rations employed contained adequate riboflavin, pantothenic and nicotinic acid to support normal gestation, lactation and growth in Yorkshire swine.

scholarly journals Enhancement of Versatile Extracellular Cellulolytic and Hemicellulolytic Enzyme Productions by Lactobacillus plantarum RI 11 Isolated from Malaysian Food Using Renewable Natural Polymers

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2607 ◽  
Author(s):  
Nursyafiqah A. Mohamad Zabidi ◽  
Hooi Ling Foo ◽  
Teck Chwen Loh ◽  
Rosfarizan Mohamad ◽  
Raha Abdul Rahim
Keyword(s):  
Lactobacillus Plantarum ◽  
Yeast Extract ◽  
Palm Kernel ◽  
Basal Medium ◽  
Optimization Approach ◽  
Palm Kernel Cake ◽  
Natural Polymers ◽  
Cell Biomass ◽  
Specific Activities ◽  
Hemicellulolytic Enzyme

Lactobacillus plantarum RI 11 was reported recently to be a potential lignocellulosic biomass degrader since it has the capability of producing versatile extracellular cellulolytic and hemicellulolytic enzymes. Thus, this study was conducted to evaluate further the effects of various renewable natural polymers on the growth and production of extracellular cellulolytic and hemicellulolytic enzymes by this novel isolate. Basal medium supplemented with molasses and yeast extract produced the highest cell biomass (log 10.51 CFU/mL) and extracellular endoglucanase (11.70 µg/min/mg), exoglucanase (9.99 µg/min/mg), β-glucosidase (10.43 nmol/min/mg), and mannanase (8.03 µg/min/mg), respectively. Subsequently, a statistical optimization approach was employed for the enhancement of cell biomass, and cellulolytic and hemicellulolytic enzyme productions. Basal medium that supplemented with glucose, molasses and soybean pulp (F5 medium) or with rice straw, yeast extract and soybean pulp (F6 medium) produced the highest cell population of log 11.76 CFU/mL, respectively. However, formulated F12 medium supplemented with glucose, molasses and palm kernel cake enhanced extracellular endoglucanase (4 folds), exoglucanase (2.6 folds) and mannanase (2.6 folds) specific activities significantly, indicating that the F12 medium could induce the highest production of extracellular cellulolytic and hemicellulolytic enzymes concomitantly. In conclusion, L. plantarum RI 11 is a promising and versatile bio-transformation agent for lignocellulolytic biomass.

The Nicotinic Acid, Biotin, and Pantothenic Acid Content of Cows' Milk

1946 ◽  
Vol 32 (1) ◽  
pp. 73-91 ◽  
Author(s):  
John M. Lawrence ◽  
B. L. Herrington ◽  
L. A. Maynard
Keyword(s):  
Nicotinic Acid ◽  
Acid Content ◽  
Pantothenic Acid

scholarly journals Intracellular symbiosis and vitamin requirements of two insects, Lasioderma serricorne and Sitodrepa panicea

1944 ◽  
Vol 132 (867) ◽  
pp. 212-221 ◽  
Keyword(s):  
Growth Rate ◽  
Nicotinic Acid ◽  
Artificial Diet ◽  
Pantothenic Acid ◽  
Lasioderma Serricorne ◽  
White Flour ◽  
Vitamin Requirements ◽  
Pure Substances ◽  
Better Than

Normal larvae of two beetles, Lasioderma serricorne and Sitodrepa panicea , and larvae freed from their intracellular symbionts have been grown on various diets. On white flour, a food deficient in vitamins of the B group, normal larvae grow very much better than sterilized larvae, while no such difference in the growth rate occurs on a diet rich in vitamins of the B group, e. g. wholemeal flour plus yeast. On an artificial diet which contains the vitamins of the B complex in pure substances, the normal Lasioderma larva grows well or fairly well in the absence of either thiamin, riboflavin, nicotinic acid, pyridoxin or pantothenic acid, and the normal Sitodrepa larva in the absence of any of these vitamins except thiamin. The sterilized larvae of both species fail to grow in the absence of any of these five vitamins. It is concluded that the intracellular symbionts of Lasioderma and Sitodrepa supply vitamins of the B group.

The Production of Niacin by Microorganisms Isolated from Fermenting Corn Meal1

1990 ◽  
Vol 53 (10) ◽  
pp. 881-882 ◽  
Author(s):  
KAOUTHER BEN AMOR ◽  
MARION L. FIELDS
Keyword(s):  
Lactobacillus Plantarum ◽  
Bacillus Licheniformis ◽  
Synthetic Medium ◽  
Microbiological Assay ◽  
Corn Meal ◽  
Natural Fermentation ◽  
Assay Medium ◽  
Assay Procedure ◽  
Pure Cultures

Twenty-eight microorganisms, which were isolated from a natural fermentation of corn meal, were screened for niacin production in Difco niacin assay medium. Bacillus licheniformis strains 6, 9, and 10 grew in the synthetic medium free from niacin, indicating that these strains are indeed able to synthesize niacin. The amount of niacin was assayed according to a microbiological assay procedure using Lactobacillus plantarum ATCC 8014 as the assay organism. The three strains of B. licheniformis produced almost the same amount of niacin. When corn meal was fermented with pure cultures of B. licheniformis strains 9 and 10, the niacin content significantly (p<0.05) increased as compared to the nonfermented corn meal. Strain 10 produced the largest quantity of niacin in fermented corn meal at pH 7 and 35°C.

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