scholarly journals Enhancement of Versatile Extracellular Cellulolytic and Hemicellulolytic Enzyme Productions by Lactobacillus plantarum RI 11 Isolated from Malaysian Food Using Renewable Natural Polymers

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2607 ◽  
Author(s):  
Nursyafiqah A. Mohamad Zabidi ◽  
Hooi Ling Foo ◽  
Teck Chwen Loh ◽  
Rosfarizan Mohamad ◽  
Raha Abdul Rahim
Keyword(s):  
Lactobacillus Plantarum ◽  
Yeast Extract ◽  
Palm Kernel ◽  
Basal Medium ◽  
Optimization Approach ◽  
Palm Kernel Cake ◽  
Natural Polymers ◽  
Cell Biomass ◽  
Specific Activities ◽  
Hemicellulolytic Enzyme

Lactobacillus plantarum RI 11 was reported recently to be a potential lignocellulosic biomass degrader since it has the capability of producing versatile extracellular cellulolytic and hemicellulolytic enzymes. Thus, this study was conducted to evaluate further the effects of various renewable natural polymers on the growth and production of extracellular cellulolytic and hemicellulolytic enzymes by this novel isolate. Basal medium supplemented with molasses and yeast extract produced the highest cell biomass (log 10.51 CFU/mL) and extracellular endoglucanase (11.70 µg/min/mg), exoglucanase (9.99 µg/min/mg), β-glucosidase (10.43 nmol/min/mg), and mannanase (8.03 µg/min/mg), respectively. Subsequently, a statistical optimization approach was employed for the enhancement of cell biomass, and cellulolytic and hemicellulolytic enzyme productions. Basal medium that supplemented with glucose, molasses and soybean pulp (F5 medium) or with rice straw, yeast extract and soybean pulp (F6 medium) produced the highest cell population of log 11.76 CFU/mL, respectively. However, formulated F12 medium supplemented with glucose, molasses and palm kernel cake enhanced extracellular endoglucanase (4 folds), exoglucanase (2.6 folds) and mannanase (2.6 folds) specific activities significantly, indicating that the F12 medium could induce the highest production of extracellular cellulolytic and hemicellulolytic enzymes concomitantly. In conclusion, L. plantarum RI 11 is a promising and versatile bio-transformation agent for lignocellulolytic biomass.

scholarly journals Indirect Method for Quantification of Cell Biomass During Solid-State Fermentation of Palm Kernel Cake Based on Protein Content

2008 ◽  
Vol 1 (4) ◽  
pp. 385-393 ◽  
Author(s):  
Suraini Abd-Aziz ◽  
Gan Siew Hung ◽  
Mohd Ali Hassan ◽  
Mohamed Ismail Abdul Kari ◽  
Noraini Samat
Keyword(s):  
Solid State ◽  
Protein Content ◽  
Solid State Fermentation ◽  
Indirect Method ◽  
Palm Kernel ◽  
Palm Kernel Cake ◽  
Cell Biomass

scholarly journals Enhancement of β-Mannanase Production by Bacillus subtilis ATCC11774 through Optimization of Medium Composition

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3516 ◽  
Author(s):  
Nor Amalina Binti Mahamad Norizan ◽  
Murni Halim ◽  
Joo Shun Tan ◽  
Sahar Abbasiliasi ◽  
Miskandar Mat Sahri ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Yeast Extract ◽  
Palm Kernel ◽  
Fermentation Medium ◽  
Luria Bertani ◽  
Medium Composition ◽  
Constant Factor ◽  
Nutrient Broth ◽  
Palm Kernel Cake ◽  
Mannanase Activity

Palm kernel cake (PKC) has been largely produced in Malaysia as one of the cheap and abundant agro-waste by-products from the palm oil industry and it contains high fiber (mannan) content. The present study aimed to produce β-mannanase by Bacillus subtilis ATCC11774 via optimization of the medium composition using palm kernel cake as substrate in semi-solid fermentation. The fermentation nutrients such as PKC, peptone, yeast extract, sodium chloride, magnesium sulphate (MgSO2), initial culture pH and temperature were screened using a Plackett-Burman design. The three most significant factors identified, PKC, peptone and NaCl, were further optimized using central composite design (CCD), a response surface methodology (RSM) approach, where yeast extract and MgSO2 were fixed as a constant factor. The maximum β-mannanase activity predicted by CCD under the optimum medium composition of 16.50 g/L PKC, 19.59 g/L peptone, 3.00 g/L yeast extract, 2.72 g/L NaCl and 0.2 g/L MgSO2 was 799 U/mL. The validated β-mannanase activity was 805.12 U/mL, which was close to the predicted β-mannanas activity. As a comparison, commercial media such as nutrient broth, M9 and Luria bertani were used for the production of β-mannanase with activities achieved at 204.16 ± 9.21 U/mL, 50.32 U/mL and 88.90 U/mL, respectively. The optimized PKC fermentation medium was four times higher than nutrient broth. Hence, it could be a potential fermentation substrate for the production of β-mannanase activity by Bacillus subtilis ATCC11774.

scholarly journals Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

2019 ◽  
Vol 20 (20) ◽  
pp. 4979 ◽  
Author(s):  
Fu Haw Lee ◽  
Suet Ying Wan ◽  
Hooi Ling Foo ◽  
Teck Chwen Loh ◽  
Rosfarizan Mohamad ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Enzyme Activities ◽  
Hydrolytic Enzyme ◽  
Palm Kernel ◽  
Cellulosic Biomass ◽  
Fermented Foods ◽  
Palm Kernel Cake ◽  
Hemicellulolytic Enzyme ◽  
Extracellular Hydrolytic Enzyme

Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass.

Feeding behavior of feedlot-finished crossbred bulls fed palm kernel cake

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Mateus de M. Lisboa ◽  
Robério R. Silva ◽  
Fabiano F. da Silva ◽  
Maria M. S. Pereira ◽  
Gabriel D. Costa ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Palm Kernel ◽  
Palm Kernel Cake

Bioconvertibility of mannan-containing polysaccharides to bioethanol: a comparative study of palm kernel cake and copra meal feedstocks

2021 ◽  
Author(s):  
Ukponobong E. Antia ◽  
Nsikak U. Stephen ◽  
Anthony A. Onilude ◽  
Itoro-obong M. Udo ◽  
Tivkaa J. Amande
Keyword(s):  
Comparative Study ◽  
Palm Kernel ◽  
Palm Kernel Cake ◽  
Copra Meal

Effects of palm kernel cake in high-concentrate diets on carcass traits and meat quality of feedlot goats

2021 ◽  
pp. 104456
Author(s):  
Liliane Oliveira da Silva ◽  
Gleidson Giordano Pinto de Carvalho ◽  
Manuela Silva Libano Tosto ◽  
Victor Guimarães Oliveira Lima ◽  
Luis Gabriel Alves Cirne Cirne ◽  
...  
Keyword(s):  
Meat Quality ◽  
Carcass Traits ◽  
Palm Kernel ◽  
Palm Kernel Cake

scholarly journals Pengaruh Pemberian Bungkil Kelapa Sawit Segar (BKS) dan Fermentasi (BKSF) Terhadap Kualitas Fisik Telur Itik

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Arif Pranata
Keyword(s):  
Shell Thickness ◽  
Mass Shell ◽  
Egg Quality ◽  
Palm Kernel ◽  
Egg Mass ◽  
Palm Kernel Cake ◽  
Dietary Treatments ◽  
Range Test ◽  
Haugh Unit

This research was conducted to utilize fresh and fermented palm kernel cake in the ducks diets on egg physical quality. One hundred and five laying ducks were randomly devided in to five diets treatments in three replications with seven laying ducks each the dietary treatments were K = control, was diet without palm kernel cake, BKS 10 and BKS 30 diets with addition 10 % and 30 % fresh palm kernel cake, BKSF 10 and BKSF 30 diets with addition 10 % and 30 % fermented palm kernel cake. The data collected were egg mass, shell thickness, Haugh Unit and yolk colour and will be analyzed by a one way classification of variance analysis (CRD), followed by testing the significant mean by Duncan’s New Multiple Range Test (DMRT). The results showed the egg mass, shell thickness, and HU had not significant different and egg mass were (64,74, 65,71, 67,22, 65,39, 63,52 g), shell thickness were (0,47, 0,49, 0,44, 0,48, 0,45 mm), Haught Unit were (86,81, 87,44, 90,94, 88,15, 88,08 %). Yolk colour had significant different between the treatment (9.31, 9,72, 9,81, 10,69, 10,74). It be concluded that the utilization 30% of fresh and fermented palm kernel cake in feed duck had not increase egg mass, shell tickness, and haugh unit, except on yolk colour. Key words :Fermentation, Egg Quality, Laying Duck, Palm kernel cake

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