scholarly journals Sustained delivery of calcium and orthophosphate ions from amorphous calcium phosphate and poly(L-lactic acid)-based electrospinning nanofibrous scaffold

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Xufeng Niu ◽  
Zhongning Liu ◽  
Feng Tian ◽  
Siqian Chen ◽  
Lei Lei ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Nanofibrous Scaffold ◽  
Sustained Delivery

scholarly journals Improved Biocompatibility of Novel Biodegradable Scaffold Composed of Poly-L-lactic Acid and Amorphous Calcium Phosphate Nanoparticles in Porcine Coronary Artery

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Dongsheng Gu ◽  
Gaoke Feng ◽  
Guanyang Kang ◽  
Xiaoxin Zheng ◽  
Yuying Bi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lactic Acid ◽  
Calcium Phosphate ◽  
Coronary Arteries ◽  
Amorphous Calcium Phosphate ◽  
Serum Levels ◽  
Porcine Coronary Artery ◽  
Biodegradable Scaffold ◽  
Biodegradable Poly ◽  
Acidic Degradation

Using poly-L-lactic acid for implantable biodegradable scaffold has potential biocompatibility issue due to its acidic degradation byproducts. We have previously reported that the addition of amorphous calcium phosphate improved poly-L-lactic acid coating biocompatibility. In the present study, poly-L-lactic acid and poly-L-lactic acid/amorphous calcium phosphate scaffolds were implanted in pig coronary arteries for 28 days. At the follow-up angiographic evaluation, no case of stent thrombosis was observed, and the arteries that were stented with the copolymer scaffold had significantly less inflammation and nuclear factor-κB expression and a greater degree of reendothelialization. The serum levels of vascular endothelial growth factor and nitric oxide, as well the expression of endothelial nitric oxide synthase and platelet-endothelial cell adhesion molecule-1, were also significantly higher. In conclusion, the addition of amorphous calcium phosphate to biodegradable poly-L-lactic acid scaffold minimizes the inflammatory response, promotes the growth of endothelial cells, and accelerates the reendothelialization of the stented coronary arteries.

scholarly journals Evaluation of Long-Term Inflammatory Responses after Implantation of a Novel Fully Bioabsorbable Scaffold Composed of Poly-L-lactic Acid and Amorphous Calcium Phosphate Nanoparticles

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Gaoke Feng ◽  
Thanh Dinh Nguyen ◽  
Xin Yi ◽  
Yongnan Lyu ◽  
Zhiyuan Lan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Calcium Phosphate ◽  
Protein Expression ◽  
Amorphous Calcium Phosphate ◽  
Inflammatory Responses ◽  
Serum Levels ◽  
Fibrosis Score ◽  
Inflammatory Score ◽  
Significant Difference

Objectives. Our previous studies have confirmed the superior biocompatibility of the poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) scaffolds compared to PLLA scaffolds at 1-month. In the present study, the long-term inflammatory responses of PLLA/ACP scaffolds in a porcine coronary model have been explored. Methods. The 24 PLLA scaffolds and 24 PLLA/ACP scaffolds were implanted into the coronary arteries of 24 miniature pigs. Serum levels of ALT, AST, and CRP were measured before operation, as well as 1-month, 6-months, 12-months, and 24-months. The vascular segments were taken for pathomorphological observation. HE staining was used for the inflammatory score and fibrosis score. Immunohistochemical staining detected positive expression indexes of MMP-9 and NF-κB. The expression of inflammation-related proteins of IL-1 and IL-6 was detected by Western Blot in surrounding tissues of scaffolds. Results. There was no significant difference between the two groups in ALT, AST, and UR at different time points (P<0.05). The inflammation score in the PLLA/ACP group was lower than that in the PLLA group at 6-months, 12-months, and 24-months (P<0.05), and the fibrosis score was reduced in the PLLA/ACP group than that in the PLLA group at 12-months and 24-months (P<0.05). The expression of MMP-9 and NF-κB in the PLLA/ACP group was significantly less than that in the PLLA group at 6-months, 12-months, and 24-months (P<0.05). The protein expression of IL-1 in the PLLA/ACP group was decreased than that in the PLLA group at 12-months and 24-months (P<0.05). Furthermore, the protein expression of IL-1 was significantly lower than that in the PLLA group at 6-months, 12-months, and 24-months (P<0.01). Conclusions. The supplement of ACP nanoparticles can effectively reduce the long-term inflammatory reaction caused by PLLA and has good safety and biocompatibility. The novel bioabsorbable PLLA/ACP scaffold provides reliable guidance for the development and clinical application of bioabsorbable scaffolds in the future.

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