scholarly journals Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Inbal Shainer ◽  
Adi Buchshtab ◽  
Thomas A. Hawkins ◽  
Stephen W. Wilson ◽  
Roger D. Cone ◽  
...  
Keyword(s):  
Bac Transgenesis

scholarly journals BAC Transgenesis in Human Embryonic Stem Cells as a Novel Tool to Define the Human Neural Lineage

Stem Cells ◽  
2009 ◽  
Vol 27 (3) ◽  
pp. 521-532 ◽  
Author(s):  
Dimitris G. Placantonakis ◽  
Mark J. Tomishima ◽  
Fabien Lafaille ◽  
Sabrina C. Desbordes ◽  
Fan Jia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Neural Lineage ◽  
Bac Transgenesis

scholarly journals Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit

2020 ◽  
Vol 9 (5) ◽  
pp. 1100-1116
Author(s):  
Binhui Zhao ◽  
Pankaj Chaturvedi ◽  
David L. Zimmerman ◽  
Andrew S. Belmont
Keyword(s):  
Single Step ◽  
Multigene Expression ◽  
Bac Transgenesis

scholarly journals A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) Transgenesis in Zebrafish

2016 ◽  
Vol 6 (4) ◽  
pp. 829-834 ◽  
Author(s):  
Fernando Fuentes ◽  
Eric Reynolds ◽  
Stephen W. Lewellis ◽  
Gayatri Venkiteswaran ◽  
Holger Knaut
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Bac Transgenesis

scholarly journals β-Globin cis-elements determine differential nuclear targeting through epigenetic modifications

2013 ◽  
Vol 203 (5) ◽  
pp. 767-783 ◽  
Author(s):  
Qian Bian ◽  
Nimish Khanna ◽  
Jurgis Alvikas ◽  
Andrew S. Belmont
Keyword(s):  
Globin Gene ◽  
Nuclear Periphery ◽  
Artificial Chromosome ◽  
Pericentric Heterochromatin ◽  
Cis Elements ◽  
Nuclear Targeting ◽  
Bac Transgenesis ◽  
Flanking Sequences ◽  
Peripheral Heterochromatin

Increasing evidence points to nuclear compartmentalization as a contributing mechanism for gene regulation, yet mechanisms for compartmentalization remain unclear. In this paper, we use autonomous targeting of bacterial artificial chromosome (BAC) transgenes to reveal cis requirements for peripheral targeting. Three peripheral targeting regions (PTRs) within an HBB BAC bias a competition between pericentric versus peripheral heterochromatin targeting toward the nuclear periphery, which correlates with increased H3K9me3 across the β-globin gene cluster and locus control region. Targeting to both heterochromatin compartments is dependent on Suv39H-mediated H3K9me3 methylation. In different chromosomal contexts, PTRs confer no targeting, targeting to pericentric heterochromatin, or targeting to the periphery. A combination of fluorescent in situ hybridization, BAC transgenesis, and knockdown experiments reveals that peripheral tethering of the endogenous HBB locus depends both on Suv39H-mediated H3K9me3 methylation over hundreds of kilobases surrounding HBB and on G9a-mediated H3K9me2 methylation over flanking sequences in an adjacent lamin-associated domain. Our results demonstrate that multiple cis-elements regulate the overall balance of specific epigenetic marks and peripheral gene targeting.

scholarly journals Establishment of an Efficient BAC Transgenesis Protocol and its Application to Functional Characterization of the Mouse Brachyury Locus

2004 ◽  
Vol 53 (4) ◽  
pp. 311-320 ◽  
Author(s):  
Kuniya ABE ◽  
Masaaki HAZAMA ◽  
Hideki KATOH ◽  
Ken-ichi YAMAMURA ◽  
Misao SUZUKI
Keyword(s):  
Functional Characterization ◽  
Bac Transgenesis
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