scholarly journals Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit

2020 ◽  
Vol 9 (5) ◽  
pp. 1100-1116
Author(s):  
Binhui Zhao ◽  
Pankaj Chaturvedi ◽  
David L. Zimmerman ◽  
Andrew S. Belmont
Keyword(s):  
Single Step ◽  
Multigene Expression ◽  
Bac Transgenesis

V884: Demonstration of a Novel Single-Step Percutaneous Access Sheath for Nephrostolithotomy: A Video Presentation

2005 ◽  
Vol 173 (4S) ◽  
pp. 240-240
Author(s):  
Premal J. Desai ◽  
David A. Hadley ◽  
Lincoln J. Maynes ◽  
D. Duane Baldwin
Keyword(s):  
Single Step ◽  
Video Presentation ◽  
Percutaneous Access ◽  
Access Sheath

Effect of Lipoprotein(a) and LDL on Plasminogen Binding to Extracellular Matrix and on Matrix-dependent Plasminogen Activation by Tissue Plasminogen Activator

1996 ◽  
Vol 75 (03) ◽  
pp. 497-502 ◽  
Author(s):  
Hadewijch L M Pekelharing ◽  
Henne A Kleinveld ◽  
Pieter F C.C.M Duif ◽  
Bonno N Bouma ◽  
Herman J M van Rijn
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Binding Sites ◽  
Umbilical Vein ◽  
Single Step ◽  
Plasminogen Activation ◽  
Structural Homology ◽  
Tissue Plasminogen ◽  
Umbilical Vein Endothelial Cells

SummaryLp(a) is an LDL-like lipoprotein plus an additional apolipoprotein apo(a). Based on the structural homology of apo(a) with plasminogen, it is hypothesized that Lp(a) interferes with fibrinolysis. Extracellular matrix (ECM) produced by human umbilical vein endothelial cells was used to study the effect of Lp(a) and LDL on plasminogen binding and activation. Both lipoproteins were isolated from the same plasma in a single step. Plasminogen bound to ECM via its lysine binding sites. Lp(a) as well as LDL were capable of competing with plasminogen binding. The degree of inhibition was dependent on the lipoprotein donor as well as the ECM donor. When Lp(a) and LDL obtained from one donor were compared, Lp(a) was always a much more potent competitor. The effect of both lipoproteins on plasminogen binding was reflected in their effect on plasminogen activation. It is speculated that Lp(a) interacts with ECM via its LDL-like lipoprotein moiety as well as via its apo(a) moiety.

Purification of Urokinase from Complex Mixtures Using Immobilized Monoclonal Antibody Against Urokinase Light Chain

1983 ◽  
Vol 49 (01) ◽  
pp. 024-027 ◽  
Author(s):  
David Vetterlein ◽  
Gary J Calton
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Culture Media ◽  
Biological Fluids ◽  
Single Step ◽  
High Yield ◽  
Step Method ◽  
Commercial Preparation ◽  
E Coli ◽  
Tissue Culture Media

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

Distribution of Aedes aegypti and Aedes albopictus in different eco zones of Thiruvananthapuram city with special reference to dengue viremia in humans

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 223-230
Author(s):  
S. Sunil Kumar ◽  
D.A. Evans ◽  
K. Muthulakshmi ◽  
T. DilipKumar ◽  
R. Heera Pillai ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Dengue Shock Syndrome ◽  
Single Step ◽  
Equal Distribution ◽  
Distribution Study ◽  
Urban Zone ◽  
High Prevalence ◽  
Vector Management ◽  
Efficient Vector

Mosquito index study of three ecologically different ecozones of the Thiruvananthapuram district, Kerala showed sharp difference on the proportionate distribution of Aedes aegypti and Aedes albopictus. Human dengue viremia (HDV) was very high in those ecozones where A.aegypti density was high and HDV was low where A.albopictus was high. In a coastal zone of Thiruvananthapuram city, A. aegypti was the most abundant vector and in a hilly, arid suburban zone, A.albopictus was the abundant vector. In the urban zone both species of mosquitoes showed equal distribution. Study on the circulating serotypes in the serum of HDV by Single step single tube Multiplex PCR showed all the four serotypes viz DENV1, DENV2, DENV3 and DENV4 in patients of Thiruvananthapuram city, which indicated the possibility of Dengue Shock Syndrome, unless there is efficient vector management. Among the four dengue serotypes, Type 1 was the most abundant virus. Abundance of microhabitats in Thiruvananthapuram city, which support A. aegypti may be the reason for high prevalence of dengue fever in the urban zone.

Monobloco – cimentação de pino e preenchimento em único passo em dente tratado endodonticamente

2020 ◽  
Vol 10 (37) ◽  
pp. 10-19
Author(s):  
Patrícia Ferronato Araújo ◽  
Ana Carolina Portes Pasmadjian ◽  
Antonio Setsuo Sakamoto Junior
Keyword(s):  
Composite Resin ◽  
Case Reports ◽  
Single Step ◽  
Resin Cement ◽  
Endodontically Treated Teeth ◽  
Posterior Teeth ◽  
Elasticity Module ◽  
Post And Core Technique ◽  
Post And Core ◽  
Root Fiber

The literature reports several materials for the restoration of endodontically treated teeth, with coronal destruction that requires an intra-root fiber post to stabilize the prosthetic part. The intra-radicular post of composite resin reinforced with fiberglass can be used as retainers in rehabilitating endodontically treated teeth due to their adhesiveness, pleasing aesthetics, and elasticity module close to the dentin less wear on the remaining structure. This clinical case reports the step-by-step application of dual resin cement (Rebilda DC Dentine - Voco) used as the cementation material of the post and material for the filling core in a single step, in an upper right lateral incisor. Moreover, it describes the advantages and indications of the post-and-core technique, also called “monobloc” which can be used in both anterior and posterior teeth. This protocol minimizes the adhesive interfaces, the chair time, and the steps of the clinical procedure.

0303 Issues in commercial application of single-step genomic BLUP for genetic evaluation in American Angus

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 144-145
Author(s):  
D. A. L. Lourenco ◽  
S. Tsuruta ◽  
B. D. Fragomeni ◽  
Y. Masuda ◽  
I. Pocrnic ◽  
...  
Keyword(s):  
Genetic Evaluation ◽  
Single Step ◽  
Commercial Application
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