scholarly journals BAC Transgenesis in Human Embryonic Stem Cells as a Novel Tool to Define the Human Neural Lineage

Stem Cells ◽  
2009 ◽  
Vol 27 (3) ◽  
pp. 521-532 ◽  
Author(s):  
Dimitris G. Placantonakis ◽  
Mark J. Tomishima ◽  
Fabien Lafaille ◽  
Sabrina C. Desbordes ◽  
Fan Jia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Neural Lineage ◽  
Bac Transgenesis

E-Cadherin-Expressing Feeder Cells Promote Neural Lineage Restriction of Human Embryonic Stem Cells

2012 ◽  
Vol 21 (1) ◽  
pp. 30-41 ◽  
Author(s):  
Rebecca N. Moore ◽  
Jocie F. Cherry ◽  
Vani Mathur ◽  
Rick Cohen ◽  
Martin Grumet ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Cells ◽  
Neural Lineage ◽  
E Cadherin

scholarly journals Soluble Amyloid Precursor Protein Induces Rapid Neural Differentiation of Human Embryonic Stem Cells

2011 ◽  
Vol 286 (27) ◽  
pp. 24264-24274 ◽  
Author(s):  
Kristine K. Freude ◽  
Mahmud Penjwini ◽  
Joy L. Davis ◽  
Frank M. LaFerla ◽  
Mathew Blurton-Jones
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Amyloid Precursor Protein ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Precursor Protein ◽  
Stem Cell Marker ◽  
Loss Of Function ◽  
Neural Lineage

Human embryonic stem cells (hESCs) offer tremendous potential for not only treating neurological disorders but also for their ability to serve as vital reagents to model and investigate human disease. To further our understanding of a key protein involved in Alzheimer disease pathogenesis, we stably overexpressed amyloid precursor protein (APP) in hESCs. Remarkably, we found that APP overexpression in hESCs caused a rapid and robust differentiation of pluripotent stem cells toward a neural fate. Despite maintenance in standard hESC media, up to 80% of cells expressed the neural stem cell marker nestin, and 65% exhibited the more mature neural marker β-3 tubulin within just 5 days of passaging. To elucidate the mechanism underlying the effects of APP on neural differentiation, we examined the proteolysis of APP and performed both gain of function and loss of function experiments. Taken together, our results demonstrate that the N-terminal secreted soluble forms of APP (in particular sAPPβ) robustly drive neural differentiation of hESCs. Our findings not only reveal a novel and intriguing role for APP in neural lineage commitment but also identify a straightforward and rapid approach to generate large numbers of neurons from human embryonic stem cells. These novel APP-hESC lines represent a valuable tool to investigate the potential role of APP in development and neurodegeneration and allow for insights into physiological functions of this protein.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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