scholarly journals Direct Evidence of Lack of Colocalisation of Fluorescently Labelled Gold Labels Used in Correlative Light Electron Microscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Benjamin T. Miles ◽  
Alexander B. Greenwood ◽  
David Benito-Alifonso ◽  
Hugh Tanner ◽  
M. Carmen Galan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Direct Evidence

Detection of Amorphous Silica in Air-Oxidized Ti3SiC2 at 500–1000°C by NMR and SIMS

2010 ◽  
Vol 434-435 ◽  
pp. 169-172 ◽  
Author(s):  
Wei Kong Pang ◽  
It Meng Low ◽  
J.V. Hanna
Keyword(s):  
Electron Microscopy ◽  
Mass Spectrometry ◽  
Transmission Electron Microscopy ◽  
Amorphous Silica ◽  
Direct Evidence ◽  
Secondary Ion Mass Spectrometry ◽  
Amorphous Sio2 ◽  
Transmission Electron ◽  
First Time ◽  
Secondary Ion

The use of secondary-ion mass spectrometry (SIMS), nuclear magnetic resonance (NMR) and transmission electron microscopy (TEM) to detect the existence of amorphous silica in Ti3SiC2 oxidised at 500–1000°C is described. The formation of an amorphous SiO2 layer and its growth in thickness with temperature was monitored using dynamic SIMS. Results of NMR and TEM verify for the first time the direct evidence of amorphous silica formation during the oxidation of Ti3SiC2 at 1000°C.

Endocytosis and storage of plasma factor V by human megakaryocytes

2005 ◽  
Vol 94 (09) ◽  
pp. 585-592 ◽  
Author(s):  
Youko Suehiro ◽  
Dragoslava Kika Veljkovic ◽  
Nola Fuller ◽  
Yasuaki Motomura ◽  
Jean Marc Massé ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Direct Evidence ◽  
Human Platelet ◽  
Factor V ◽  
Platelet Factor ◽  
Exogenous Protein ◽  
Plasma Factor ◽  
And Storage

SummaryFactor V is an essential coagulation cofactor that circulates in plasma and platelet α-granules where it is stored complexed to multimerin 1 (MMRN1). To gain insights into the origin and processing of human platelet factor V, and factor V-MMRN1 complexes, we studied factor V in cultured megakaryocytes. Factor V mRNA was detected in all megakaryocyte cultures. However, like albumin, IgG and fibrinogen, factorV protein was detectable only in megakaryocytes cultured with exogenous protein. The amount of factor V associated with megakaryocytes was influenced by the exogenous factorV concentration. Similar to platelet factor V, megakaryocyte factor V was proteolyzed and complexed with megakaryocyte-synthesized MMRN1. With secretagogues, megakaryocytes released factorV, IgG, fibrinogen and MMRN1. Immunofluorescent and electron microscopy confirmed factorV uptake by endocytosis and its trafficking to megakaryocyte α-granules. These data provide direct evidence that human megakaryocytes process plasma-derived factor V into α-granules and generate factor V-MMRN1 complexes from endogenously and exogenously synthesized proteins.

scholarly journals Cell Fusion Contributes to Pericyte Formation after Stroke

2008 ◽  
Vol 29 (3) ◽  
pp. 480-485 ◽  
Author(s):  
Marina Piquer-Gil ◽  
José M García-Verdugo ◽  
Ivan Zipancic ◽  
María J Sánchez ◽  
Manuel Álvarez-Dolado
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Cell Fusion ◽  
Basal Lamina ◽  
Direct Evidence ◽  
Vascular Tissue ◽  
Mouse Bone Marrow ◽  
Mural Cells ◽  
Bone Marrow Derived Cells ◽  
Bone Marrow Chimeras

Recent reports have shown that bone marrow-derived cells (BMDCs) contribute to the formation of vasculature after stroke. However, the mechanism by which mural cells are formed from BMDC remains elusive. Here, we provide direct evidence that the cell fusion process contributes to the formation of pericytes after stroke. We generated mouse bone marrow chimeras using a cre/lox system that allows the detection of fusion events by X-gal staining. In these mice, we detected X-gal-positive cells that expressed vimentin and desmin, specific markers of mature murine pericytes. Electron microscopy confirmed that fused cells possessed basal lamina and characteristics of pericytes. Furthermore, induction of stroke increased significantly the presence of fused cells in the ischemic area. These cells expressed markers of developing pericytes such as NG2. We conclude that cell fusion participates actively in the generation of vascular tissue through pericyte formation under normal as well as pathologic conditions.

Effect of CuO on the Thermal Stability of Polyaniline Nanofibers

2014 ◽  
Vol 12 (1) ◽  
pp. 215-221 ◽  
Author(s):  
Mohammad Mizanur Rahman Khan ◽  
Yee Keat Wee ◽  
Wan Ahmad Kamil Mahmood
Keyword(s):  
Electron Microscopy ◽  
Thermal Stability ◽  
Composite Nanofibers ◽  
Direct Evidence ◽  
Synthesis Process ◽  
Polyaniline Nanofibers ◽  
Pani Nanofibers ◽  
Transmission Electron ◽  
Thermal Stability Of ◽  
Scanning Electron

Abstract Direct evidence of the control of thermal stability of polyaniline (PANI) nanofibers is reported by systematically varying the loadings of CuO in the synthesis process. The addition of Cu was maintained in the range from 0.005 g to 1.0 g. The freshly prepared PANI-CuO composite nanofibers were investigated by means of field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermogravimetric analysis (TGA). TGA data showed that the thermal stability of PANI nanofibers was greatly improved when the CuO were added, except for the highest addition of CuO (1.00 g). We also found that 0.01 g loading of CuO is favorable to obtain comparatively more thermally stable PANI than the other amount. The degradation of PANI chains started at 360°C for the PANI-CuO composites obtained at 0.01 g CuO addition, which is the highest temperature compared to PANI and the samples synthesized at other amount of CuO loadings.

Strain-Field Induced Crosshatch Formation During Molecular Beam Epitaxy of InGaAs/GaAs Films

1993 ◽  
Vol 312 ◽  
Author(s):  
X. C. Zhou ◽  
J. Jiang ◽  
A. Y. Du ◽  
J. W. Zhao ◽  
S. M. Mu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Molecular Beam Epitaxy ◽  
Molecular Beam ◽  
Direct Evidence ◽  
Surface Strain ◽  
Misfit Dislocations ◽  
Strain Fields ◽  
Transmission Electron ◽  
Gaas Films ◽  
Reflection Electron Microscopy

AbstractUsing reflection electron microscopy (REM), transmission electron microscopy (TEM), and Nomarski optical microscopy we obtained direct evidence that local surface strain-fields, originated from misfit dislocations, are responsible for the formation of morphological crosshatches during molecular beam epitaxy of lattice mismatched InGaAs/GaAs layers. A mechanism is proposed to correlate the formation of the crosshatched patterns with the variation of the growth rate across the epitaxial surface under the perturbation of network shaped strain-fields in the surface.

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