scholarly journals Cell Fusion Contributes to Pericyte Formation after Stroke

2008 ◽  
Vol 29 (3) ◽  
pp. 480-485 ◽  
Author(s):  
Marina Piquer-Gil ◽  
José M García-Verdugo ◽  
Ivan Zipancic ◽  
María J Sánchez ◽  
Manuel Álvarez-Dolado
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Cell Fusion ◽  
Basal Lamina ◽  
Direct Evidence ◽  
Vascular Tissue ◽  
Mouse Bone Marrow ◽  
Mural Cells ◽  
Bone Marrow Derived Cells ◽  
Bone Marrow Chimeras

Recent reports have shown that bone marrow-derived cells (BMDCs) contribute to the formation of vasculature after stroke. However, the mechanism by which mural cells are formed from BMDC remains elusive. Here, we provide direct evidence that the cell fusion process contributes to the formation of pericytes after stroke. We generated mouse bone marrow chimeras using a cre/lox system that allows the detection of fusion events by X-gal staining. In these mice, we detected X-gal-positive cells that expressed vimentin and desmin, specific markers of mature murine pericytes. Electron microscopy confirmed that fused cells possessed basal lamina and characteristics of pericytes. Furthermore, induction of stroke increased significantly the presence of fused cells in the ischemic area. These cells expressed markers of developing pericytes such as NG2. We conclude that cell fusion participates actively in the generation of vascular tissue through pericyte formation under normal as well as pathologic conditions.

scholarly journals Characterization of cell fusion in an experimental mouse model of endometriosis†

2018 ◽  
Vol 100 (2) ◽  
pp. 390-397 ◽  
Author(s):  
A Tal ◽  
R Tal ◽  
S Shaikh ◽  
S Gidicsin ◽  
R Mamillapalli ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mouse Model ◽  
Cell Fusion ◽  
Bone Marrow Cells ◽  
Low Frequency ◽  
Uterine Cavity ◽  
Stromal Compartment ◽  
Experimental Mouse Model ◽  
Experimental Mouse ◽  
Bone Marrow Derived Cells

Abstract Cell fusion is involved in the development of some adult organs, is implicated in the pathogenesis of specific types of cancer, and is known to participate in repair/regeneration processes mediated by bone-marrow-derived cells (BMDCs). Endometriosis is a disease characterized by growth of functional endometrial tissue outside of the uterine cavity. Endometriosis shares some molecular properties with cancer and BMDCs home to endometriosis lesions in a mouse model. Our objective was to determine if cell fusion can occur in endometriosis and establish whether bone-marrow-derived cells participate in cell fusion events in lesions. We employed a Cre-Lox system to identify cell fusion events in a mouse model of endometriosis. Fused cells were detected in endometriotic lesions, albeit at a low frequency (∼1 in 400 cells), localized to the stromal compartment, and displayed restricted proliferation. Using 5-fluorouracil-based nongonadotoxic bone marrow transplantation model, we demonstrate that bone marrow cells represent a principal cell source for fusion events in lesions. Cell fusion progeny uniformly lacked expression of selected markers of hematopoietic, endothelial, and epithelial markers, though they expressed the mesenchymal/stromal markers Sca-1 and CD29. This study is the first to describe the phenomenon of cell fusion in endometriosis and points to a mesenchymal population derived from cell fusion events with limited proliferative activity, properties previously attributed to endometrial stem cells. Their putative role in the pathogenesis of the disease remains to be elucidated.

scholarly journals Ultrastructural study of spontaneous bone marrow rosette-forming cells.

1977 ◽  
Vol 72 (3) ◽  
pp. 773-777 ◽  
Author(s):  
B Nabarra ◽  
J Charreire ◽  
J F Cavellier ◽  
J F Bach
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bone Marrow ◽  
T Cell ◽  
Ultrastructural Study ◽  
Target Cells ◽  
Mouse Bone Marrow ◽  
Thymic Hormone ◽  
Mouse Lymphocytes ◽  
Scanning Electron

Mouse bone marrow contains spontaneous rosette-forming cells (RFC) which include more than 70% T-cell precursors, as assessed by their transformation into theta-positive cells after incubation with thymic hormone. Such spontaneous RFC, examined in C57B1/6 mouse bone marrow by electron and scanning electron microscopy, have consistently been shown to be small, inactive mouse lymphocytes when macrophages have been eliminated by cell preincubation. These data suggest that thymic hormone target cells include small quiescent lymphocytes.

CHARACTERIZATION OF MATURATION AND FUNCTION OF NATURAL KILLER CELLS IN XENOGENEIC (RAT ± MOUSE) BONE MARROW CHIMERAS

1993 ◽  
Vol 55 (2) ◽  
pp. 355-360 ◽  
Author(s):  
MARCEL R. M. VAN DEN BRINK ◽  
SAMUEL W. FRENCH ◽  
TRACI P. BECK ◽  
MARY LYNN HRONAKES ◽  
SHERRY M. WREN ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Mouse Bone Marrow ◽  
Killer Cells ◽  
And Function ◽  
Bone Marrow Chimeras

scholarly journals Cell Fusion Reprogramming Leads to a Specific Hepatic Expression Pattern during Mouse Bone Marrow Derived Hepatocyte Formation In Vivo

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33945 ◽  
Author(s):  
Oscar Quintana-Bustamante ◽  
Esther Grueso ◽  
Ramon Garcia-Escudero ◽  
Elvira Arza ◽  
Alberto Alvarez-Barrientos ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Expression Pattern ◽  
Cell Fusion ◽  
Mouse Bone Marrow ◽  
Hepatic Expression

Immune function in fully allogeneic mouse bone marrow chimeras

1981 ◽  
Vol 19 (2) ◽  
pp. 268-283 ◽  
Author(s):  
Susan E. Krown ◽  
Richard Coico ◽  
Margrit P. Scheid ◽  
Gabriel Fernandes ◽  
Robert A. Good
Keyword(s):  
Bone Marrow ◽  
Immune Function ◽  
Mouse Bone Marrow ◽  
Bone Marrow Chimeras

scholarly journals Mild Cerebellar Neurodegeneration of Aged Heterozygous PCD Mice Increases Cell Fusion of Purkinje and Bone Marrow-Derived Cells

2012 ◽  
Vol 21 (7) ◽  
pp. 1595-1602 ◽  
Author(s):  
David Díaz ◽  
Javier S. Recio ◽  
Eduardo Weruaga ◽  
Jose R. Alonso
Keyword(s):  
Bone Marrow ◽  
Cell Fusion ◽  
Bone Marrow Derived Cells
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