scholarly journals Two-photon excited fluorescence of intrinsic fluorophores enables label-free assessment of adipose tissue function

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlo Amadeo Alonzo ◽  
Sevasti Karaliota ◽  
Dimitra Pouli ◽  
Zhiyi Liu ◽  
Katia P. Karalis ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Label Free ◽  
Two Photon ◽  
Intrinsic Fluorophores

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Markus Seeger ◽  
Christoph Dehner ◽  
Dominik Jüstel ◽  
Vasilis Ntziachristos
Keyword(s):  
Wound Healing ◽  
Label Free ◽  
Third Harmonic ◽  
Two Photon ◽  
Non Invasive ◽  
Photon Excitation ◽  
Free Mode ◽  
Spatio Temporal ◽  
And Function

AbstractThe non-invasive investigation of multiple biological processes remains a methodological challenge as it requires capturing different contrast mechanisms, usually not available with any single modality. Intravital microscopy has played a key role in dynamically studying biological morphology and function, but it is generally limited to resolving a small number of contrasts, typically generated by the use of transgenic labels, disturbing the biological system. We introduce concurrent 5-modal microscopy (Co5M), illustrating a new concept for label-free in vivo observations by simultaneously capturing optoacoustic, two-photon excitation fluorescence, second and third harmonic generation, and brightfield contrast. We apply Co5M to non-invasively visualize multiple wound healing biomarkers and quantitatively monitor a number of processes and features, including longitudinal changes in wound shape, microvascular and collagen density, vessel size and fractality, and the plasticity of sebaceous glands. Analysis of these parameters offers unique insights into the interplay of wound closure, vasodilation, angiogenesis, skin contracture, and epithelial reformation in space and time, inaccessible by other methods. Co5M challenges the conventional concept of biological observation by yielding multiple simultaneous parameters of pathophysiological processes in a label-free mode.

In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser

2020 ◽  
Vol 45 (10) ◽  
pp. 2704
Author(s):  
Ting Wu ◽  
Jiuling Liao ◽  
Jia Yu ◽  
Yufeng Gao ◽  
Hui Li ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Label Free ◽  
Two Photon ◽  
Photon Excitation ◽  
Femtosecond Fiber Laser ◽  
Two Photon Excitation

scholarly journals Label-free near-infrared reflectance microscopy as a complimentary tool for two-photon fluorescence brain imaging

2015 ◽  
Vol 6 (11) ◽  
pp. 4483 ◽  
Author(s):  
Anna Letizia Allegra Mascaro ◽  
Irene Costantini ◽  
Emilia Margoni ◽  
Giulio Iannello ◽  
Alessandro Bria ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Near Infrared ◽  
Infrared Reflectance ◽  
Label Free ◽  
Near Infrared Reflectance ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Reflectance Microscopy ◽  
Photon Fluorescence

scholarly journals High-resolution, label-free two-photon imaging of diseased human corneas

2018 ◽  
Vol 23 (03) ◽  
pp. 1 ◽  
Author(s):  
Ana Batista ◽  
Hans Georg Breunig ◽  
Aisada König ◽  
Andreas Schindele ◽  
Tobias Hager ◽  
...  
Keyword(s):  
High Resolution ◽  
Label Free ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging
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