Label free two photon imaging to assess tissue morphology and function

2021 ◽  
Author(s):  
Irene Georgakoudi
Keyword(s):  
Label Free ◽  
Two Photon ◽  
Tissue Morphology ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
And Function

scholarly journals High-resolution, label-free two-photon imaging of diseased human corneas

2018 ◽  
Vol 23 (03) ◽  
pp. 1 ◽  
Author(s):  
Ana Batista ◽  
Hans Georg Breunig ◽  
Aisada König ◽  
Andreas Schindele ◽  
Tobias Hager ◽  
...  
Keyword(s):  
High Resolution ◽  
Label Free ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Optical changes in THP-1 macrophage metabolism in response to pro- and anti-inflammatory stimuli reported by label-free two-photon imaging

2020 ◽  
Vol 25 (01) ◽  
pp. 1 ◽  
Author(s):  
Isabel S. Smokelin ◽  
Craig Mizzoni ◽  
Josh Erndt-Marino ◽  
David Kaplan ◽  
Irene Georgakoudi
Keyword(s):  
Label Free ◽  
Two Photon ◽  
Inflammatory Stimuli ◽  
Photon Imaging ◽  
Anti Inflammatory ◽  
Two Photon Imaging

Multiparametric, label-free two-photon imaging of metabolic function (Conference Presentation)

2020 ◽  
Author(s):  
Irene Georgakoudi ◽  
Dimitra Pouli ◽  
Zhiyi Liu ◽  
Yang Zhang ◽  
Christopher Polleys
Keyword(s):  
Metabolic Function ◽  
Label Free ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

In vivo two-photon imaging of hippocampal circuit structure and function in mice

2010 ◽  
Vol 68 ◽  
pp. e446
Author(s):  
Masaaki Sato ◽  
Masako Kawano ◽  
Junichi Nakai ◽  
Yasunori Hayashi
Keyword(s):  
Structure And Function ◽  
Two Photon ◽  
Circuit Structure ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
And Function

scholarly journals Synaptic Structure and Function in the Mouse Somatosensory Cortex during Chronic Pain:In VivoTwo-Photon Imaging

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Sun Kwang Kim ◽  
Kei Eto ◽  
Junichi Nabekura
Keyword(s):  
Somatosensory Cortex ◽  
Structure And Function ◽  
Imaging Studies ◽  
Two Photon ◽  
Synaptic Structure ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
And Function ◽  
The Brain

Recent advances in two-photon microscopy and fluorescence labeling techniques have enabled us to directly see the structural and functional changes in neurons and glia, and even at synapses, in the brain of living animals. Long-termin vivotwo-photon imaging studies have shown that some postsynaptic dendritic spines in the adult cortex are rapidly eliminated or newly generated, in response to altered sensory input or synaptic activity, resulting in experience/activity-dependent rewiring of neuronal circuits.In vivoCa2+imaging studies have revealed the distinct, input-specific response patterns of excitatory neurons in the brain. These updatedin vivoapproaches are just beginning to be used for the study of pathophysiological mechanisms of chronic diseases. In this paper, we introduce recentin vivotwo-photon imaging studies demonstrating how plastic changes in synaptic structure and function of the mouse somatosensory cortex, following peripheral injury, contribute to chronic pain conditions, like neuropathic and inflammatory pain.

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