scholarly journals Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Dehui Xu ◽  
Biqing Wang ◽  
Yujing Xu ◽  
Zeyu Chen ◽  
Qinjie Cui ◽  
...  
Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4288
Author(s):  
Fernanda Malhão ◽  
Ana Catarina Macedo ◽  
Carla Costa ◽  
Eduardo Rocha ◽  
Alice Abreu Ramos

Fucoxanthin (Fx) is a carotenoid derived from marine organisms that exhibits anticancer activities. However, its role as a potential drug adjuvant in breast cancer (BC) treatment is still poorly explored. Firstly, this study investigated the cytotoxic effects of Fx alone and combined with doxorubicin (Dox) and cisplatin (Cis) on a panel of 2D-cultured BC cell lines (MCF7, SKBR3 and MDA-MB-231) and one non-tumoral cell line (MCF12A). Fucoxanthin induced cytotoxicity against all the cell lines and potentiated Dox cytotoxic effects towards the SKBR3 and MDA-MB-231 cells. The combination triggering the highest cytotoxicity (Fx 10 µM + Dox 1 µM in MDA-MB-231) additionally showed significant induction of cell death and genotoxic effects, relative to control. In sequence, the same combination was tested on 3D cultures using a multi-endpoint approach involving bioactivity assays and microscopy techniques. Similar to 2D cultures, the combination of Fx and Dox showed higher cytotoxic effects on 3D cultures compared to the isolated compounds. Furthermore, this combination increased the number of apoptotic cells, decreased cell proliferation, and caused structural and ultrastructural damages on the 3D models. Overall, our findings suggest Fx has potential to become an adjuvant for Dox chemotherapy regimens in BC treatment.


2020 ◽  
Vol 17 (12) ◽  
pp. 1795-1802
Author(s):  
Dongli Li ◽  
Yuran Ma ◽  
Wenfeng Liu ◽  
Xiang Ren ◽  
Min Chen ◽  
...  

Author(s):  
Roya Karimi ◽  
Zahra Barabadi ◽  
Bagher Larijani ◽  
Gholamreza Tavoosidana ◽  
Nasrin Lotfibakhshaiesh ◽  
...  

2017 ◽  
Vol 67 (3-4) ◽  
pp. 327-342 ◽  
Author(s):  
Anne-Helen Lutter ◽  
Jenny Scholka ◽  
Heiko Richter ◽  
Ursula Anderer

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0221942 ◽  
Author(s):  
Stefania Vernazza ◽  
Sara Tirendi ◽  
Sonia Scarfì ◽  
Mario Passalacqua ◽  
Francesco Oddone ◽  
...  

2010 ◽  
Vol 54 (4) ◽  
pp. 46 ◽  
Author(s):  
M. Riccio ◽  
E. Resca ◽  
T. Maraldi ◽  
A. Pisciotta ◽  
A. Ferrari ◽  
...  

Sensors ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 32045-32055 ◽  
Author(s):  
Tao Wang ◽  
Ryan Green ◽  
Rajesh Nair ◽  
Mark Howell ◽  
Subhra Mohapatra ◽  
...  

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1910
Author(s):  
Csilla Sebők ◽  
Patrik Tráj ◽  
Júlia Vörösházi ◽  
Máté Mackei ◽  
Márton Papp ◽  
...  

The liver with resident tissue macrophages is the site of vivid innate immunity, activated also by pathogen-associated molecular patterns (PAMPs) leaking through the intestinal barrier. As gut-derived inflammatory diseases are of outstanding importance in broiler chickens, the present study aimed to establish a proper hepatic inflammatory model by comparing the action of different PAMPs from poultry pathogens on chicken 2D and 3D primary hepatocyte—non-parenchymal cell co-cultures, the latter newly developed with a magnetic bioprinting method. The cultures were challenged by the bacterial endotoxins lipopolysaccharide (LPS) from Escherichia coli, lipoteichoic acid (LTA) from Staphylococcus aureus and by enterotoxin (ETxB) from Escherichia coli, Salmonella Typhimurium derived flagellin, phorbol myristate acetate (PMA) as a model proinflammatory agent and polyinosinic polycytidylic acid (poly I:C) for mimicking viral RNA exposure. Cellular metabolic activity was assessed with the CCK-8 test, membrane damage was monitored with the lactate dehydrogenase (LDH) leakage assay and interleukin-6 and -8 (Il-6 and -8) concentrations were measured in cell culture medium with a chicken specific ELISA. Both LPS and LTA increased the metabolic activity of the 3D cultures, concomitantly decreasing the LDH leakage, while in 2D cultures ETxB stimulated, PMA and poly I:C depressed the metabolic activity. Based on the moderately increased extracellular LDH activity, LTA seemed to diminish cell membrane integrity in 2D and poly I:C in both cell culture models. The applied endotoxins remarkably reduced the IL-8 release of 3D cultured cells, suggesting the effective metabolic adaptation and the presumably initiated anti-inflammatory mechanisms of the 3D spheroids. Notwithstanding that the IL-6 and IL-8 production of 2D cells was mostly not influenced by the endotoxins used, only the higher LTA dose was capable to evoke an IL-8 surge. Flagellin, PMA and poly I:C exerted proinflammatory action in certain concentrations in both 2D and 3D cultures, reflected by the increased cellular IL-6 release. Based on these data, LTA, flagellin, PMA and poly I:C can be considered as potent candidates to induce inflammation in chicken primary hepatic cell cultures, while LPS failed to trigger proinflammatory cytokine production, suggesting the relatively high tolerance of avian liver cells to certain bacterial endotoxins. These results substantiate that the established 3D co-cultures seemed to be proper tools for testing potential proinflammatory molecules; however, the remarkable differences between 2D and 3D models should be addressed and further studied.


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