scholarly journals Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures

2010 ◽  
Vol 54 (4) ◽  
pp. 46 ◽  
Author(s):  
M. Riccio ◽  
E. Resca ◽  
T. Maraldi ◽  
A. Pisciotta ◽  
A. Ferrari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
3D Cultures ◽  
Human Dental Pulp ◽  
Mineralized Matrix ◽  
2D And 3D

scholarly journals Blockade of TLR2 Inhibits Porphyromonas gingivalis Suppression of Mineralized Matrix Formation by Human Dental Pulp Stem Cells

2011 ◽  
Vol 37 (6) ◽  
pp. 812-818 ◽  
Author(s):  
Valerie Tom-Kun Yamagishi ◽  
Calvin D. Torneck ◽  
Shimon Friedman ◽  
George T.-J. Huang ◽  
Michael Glogauer
Keyword(s):  
Stem Cells ◽  
Porphyromonas Gingivalis ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp ◽  
Mineralized Matrix

Differential mineralization of human dental pulp stem cells on diverse polymers

2018 ◽  
Vol 63 (3) ◽  
pp. 261-269 ◽  
Author(s):  
Christian Apel ◽  
Patricia Buttler ◽  
Jochen Salber ◽  
Anandhan Dhanasingh ◽  
Sabine Neuss
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Hyaluronic Acid ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Specific Cell ◽  
Alizarin Red ◽  
Alp Activity ◽  
Human Dental Pulp ◽  
Mineralized Matrix

Abstract In tissue engineering, biomaterials are used as scaffolds for spatial distribution of specific cell types. Biomaterials can potentially influence cell proliferation and extracellular matrix formation, both in positive and negative ways. The aim of the present study was to investigate and compare mineralized matrix production of human dental pulp stem cells (DPSC), cultured on 17 different well-characterized polymers. Osteogenic differentiation of DPSC was induced for 21 days on biomaterials using dexamethasone, L-ascorbic-acid-2-phosphate, and sodium β-glycerophosphate. Success of differentiation was analyzed by quantitative RealTime PCR, alkaline phosphatase (ALP) activity, and visualization of calcium accumulations by alizarin red staining with subsequent quantification by colorimetric method. All of the tested biomaterials of an established biomaterial bank enabled a mineralized matrix formation of the DPSC after osteoinductive stimulation. Mineralization on poly(tetrafluoro ethylene) (PTFE), poly(dimethyl siloxane) (PDMS), Texin, LT706, poly(epsilon-caprolactone) (PCL), polyesteramide type-C (PEA-C), hyaluronic acid, and fibrin was significantly enhanced (p<0.05) compared to standard tissue culture polystyrene (TCPS) as control. In particular, PEA-C, hyaluronic acid, and fibrin promoted superior mineralization values. These results were confirmed by ALP activity on the same materials. Different biomaterials differentially influence the differentiation and mineralized matrix formation of human DPSC. Based on the present results, promising biomaterial candidates for bone-related tissue engineering applications in combination with DPSC can be selected.

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Methylglyoxal-Dependent Glycative Stress Is Prevented by the Natural Antioxidant Oleuropein in Human Dental Pulp Stem Cells through Nrf2/Glo1 Pathway

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 716
Author(s):  
Simona Delle Delle Monache ◽  
Fanny Pulcini ◽  
Roberta Frosini ◽  
Vincenzo Mattei ◽  
Vincenzo Nicola Talesa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Great Promise ◽  
Oral Diseases ◽  
Bioactive Component ◽  
Glycation End Products ◽  
Abnormal Accumulation ◽  
Synthetic Agents ◽  
Human Dental Pulp

Methylglyoxal (MG) is a potent precursor of glycative stress (abnormal accumulation of advanced glycation end products, AGEs), a relevant condition underpinning the etiology of several diseases, including those of the oral cave. At present, synthetic agents able to trap MG are known; however, they have never been approved for clinical use because of their severe side effects. Hence, the search of bioactive natural scavengers remains a sector of strong research interest. Here, we investigated whether and how oleuropein (OP), the major bioactive component of olive leaf, was able to prevent MG-dependent glycative stress in human dental pulp stem cells (DPSCs). The cells were exposed to OP at 50 µM for 24 h prior to the administration of MG at 300 µM for additional 24 h. We found that OP prevented MG-induced glycative stress and DPSCs impairment by restoring the activity of Glyoxalase 1 (Glo1), the major detoxifying enzyme of MG, in a mechanism involving the redox-sensitive transcription factor Nrf2. Our results suggest that OP holds great promise for the development of preventive strategies for MG-derived AGEs-associated oral diseases and open new paths in research concerning additional studies on the protective potential of this secoiridoid.

scholarly journals Cdc42 is essential for the polarized movement and adhesion of human dental pulp stem cells

2018 ◽  
Vol 85 ◽  
pp. 104-112 ◽  
Author(s):  
Mingwei Li ◽  
Liang Ma ◽  
Bing Song ◽  
Dingyi Yu ◽  
Min Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp
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