Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures

2017 ◽  
Vol 67 (3-4) ◽  
pp. 327-342 ◽  
Author(s):  
Anne-Helen Lutter ◽  
Jenny Scholka ◽  
Heiko Richter ◽  
Ursula Anderer
Keyword(s):  
Human Chondrocytes ◽  
Tetrazolium Salts ◽  
3D Cultures ◽  
2D And 3D

scholarly journals Fucoxanthin Holds Potential to Become a Drug Adjuvant in Breast Cancer Treatment: Evidence from 2D and 3D Cell Cultures

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4288
Author(s):  
Fernanda Malhão ◽  
Ana Catarina Macedo ◽  
Carla Costa ◽  
Eduardo Rocha ◽  
Alice Abreu Ramos
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
3D Models ◽  
Anticancer Activities ◽  
Cytotoxic Effects ◽  
3D Cultures ◽  
3D Cell Cultures ◽  
Tumoral Cell ◽  
Microscopy Techniques ◽  
2D And 3D

Fucoxanthin (Fx) is a carotenoid derived from marine organisms that exhibits anticancer activities. However, its role as a potential drug adjuvant in breast cancer (BC) treatment is still poorly explored. Firstly, this study investigated the cytotoxic effects of Fx alone and combined with doxorubicin (Dox) and cisplatin (Cis) on a panel of 2D-cultured BC cell lines (MCF7, SKBR3 and MDA-MB-231) and one non-tumoral cell line (MCF12A). Fucoxanthin induced cytotoxicity against all the cell lines and potentiated Dox cytotoxic effects towards the SKBR3 and MDA-MB-231 cells. The combination triggering the highest cytotoxicity (Fx 10 µM + Dox 1 µM in MDA-MB-231) additionally showed significant induction of cell death and genotoxic effects, relative to control. In sequence, the same combination was tested on 3D cultures using a multi-endpoint approach involving bioactivity assays and microscopy techniques. Similar to 2D cultures, the combination of Fx and Dox showed higher cytotoxic effects on 3D cultures compared to the isolated compounds. Furthermore, this combination increased the number of apoptotic cells, decreased cell proliferation, and caused structural and ultrastructural damages on the 3D models. Overall, our findings suggest Fx has potential to become an adjuvant for Dox chemotherapy regimens in BC treatment.

scholarly journals Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems

2017 ◽  
Vol 14 (2) ◽  
pp. 103-112 ◽  
Author(s):  
Suhaeb A. Mahmod ◽  
Simmrat Snigh ◽  
Ivan Djordjevic ◽  
Yong Mei Yee ◽  
Rohana Yusof ◽  
...  
Keyword(s):  
3D Culture ◽  
Human Chondrocytes ◽  
Culture Systems ◽  
Chondrogenic Phenotype ◽  
2D And 3D

scholarly journals Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures

2020 ◽  
Vol 17 (12) ◽  
pp. 1795-1802
Author(s):  
Dongli Li ◽  
Yuran Ma ◽  
Wenfeng Liu ◽  
Xiang Ren ◽  
Min Chen ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
3D Cultures ◽  
Pancreatic Cancer Cells ◽  
2D And 3D

Comparison of insulin secretion by transduced adipose‐derived and endometrial‐derived stem cells in 2D and 3D cultures on fibrin scaffold

2020 ◽  
Author(s):  
Roya Karimi ◽  
Zahra Barabadi ◽  
Bagher Larijani ◽  
Gholamreza Tavoosidana ◽  
Nasrin Lotfibakhshaiesh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Insulin Secretion ◽  
3D Cultures ◽  
Fibrin Scaffold ◽  
2D And 3D

scholarly journals 2D- and 3D-cultures of human trabecular meshwork cells: A preliminary assessment of an in vitro model for glaucoma study

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0221942 ◽  
Author(s):  
Stefania Vernazza ◽  
Sara Tirendi ◽  
Sonia Scarfì ◽  
Mario Passalacqua ◽  
Francesco Oddone ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
In Vitro Model ◽  
Preliminary Assessment ◽  
3D Cultures ◽  
Trabecular Meshwork Cells ◽  
Vitro Model ◽  
2D And 3D

scholarly journals Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures

2010 ◽  
Vol 54 (4) ◽  
pp. 46 ◽  
Author(s):  
M. Riccio ◽  
E. Resca ◽  
T. Maraldi ◽  
A. Pisciotta ◽  
A. Ferrari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
3D Cultures ◽  
Human Dental Pulp ◽  
Mineralized Matrix ◽  
2D And 3D

scholarly journals Surface Acoustic Waves (SAW)-Based Biosensing for Quantification of Cell Growth in 2D and 3D Cultures

Sensors ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 32045-32055 ◽  
Author(s):  
Tao Wang ◽  
Ryan Green ◽  
Rajesh Nair ◽  
Mark Howell ◽  
Subhra Mohapatra ◽  
...  
Keyword(s):  
Cell Growth ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
3D Cultures ◽  
2D And 3D
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