scholarly journals Lactate Chemical Exchange Saturation Transfer (LATEST) Imaging in vivo: A Biomarker for LDH Activity

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Catherine DeBrosse ◽  
Ravi Prakash Reddy Nanga ◽  
Puneet Bagga ◽  
Kavindra Nath ◽  
Mohammad Haris ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Magnetic Resonance ◽  
Musculoskeletal Diseases ◽  
Chemical Exchange ◽  
Radioactive Isotopes ◽  
Chemical Exchange Saturation Transfer ◽  
Resonance Spectroscopy ◽  
Multiple Quantum ◽  
Saturation Transfer ◽  
Wide Range

Abstract Non-invasive imaging of lactate is of enormous significance in cancer and metabolic disorders where glycolysis dominates. Here, for the first time, we describe a chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) method (LATEST), based on the exchange between lactate hydroxyl proton and bulk water protons to image lactate with high spatial resolution. We demonstrate the feasibility of imaging lactate with LATEST in lactate phantoms under physiological conditions, in a mouse model of lymphoma tumors and in skeletal muscle of healthy human subjects pre- and post-exercise. The method is validated by measuring LATEST changes in lymphoma tumors pre- and post-infusion of pyruvate and correlating them with lactate determined from multiple quantum filtered proton magnetic resonance spectroscopy (SEL-MQC 1H-MRS). Similarly, dynamic LATEST changes in exercising human skeletal muscle are correlated with lactate determined from SEL-MQC 1H-MRS. The LATEST method does not involve injection of radioactive isotopes or labeled metabolites. It has over two orders of magnitude higher sensitivity compared to conventional 1H-MRS. It is anticipated that this technique will have a wide range of applications including diagnosis and evaluation of therapeutic response of cancer, diabetes, cardiac and musculoskeletal diseases. The advantages of LATEST over existing methods and its potential challenges are discussed.

scholarly journals pH Mapping of Skeletal Muscle by Chemical Exchange Saturation Transfer (CEST) Imaging

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2610
Author(s):  
Yu-Wen Chen ◽  
Hong-Qing Liu ◽  
Qi-Xuan Wu ◽  
Yu-Han Huang ◽  
Yu-Ying Tung ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Magnetic Resonance ◽  
Ph Value ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Resonance Spectroscopy ◽  
Saturation Transfer ◽  
Ph Changes ◽  
Cest Imaging

Magnetic resonance imaging (MRI) is extensively used in clinical and basic biomedical research. However, MRI detection of pH changes still poses a technical challenge. Chemical exchange saturation transfer (CEST) imaging is a possible solution to this problem. Using saturation transfer, alterations in the exchange rates between the solute and water protons because of small pH changes can be detected with greater sensitivity. In this study, we examined a fatigued skeletal muscle model in electrically stimulated mice. The measured CEST signal ratio was between 1.96 ppm and 2.6 ppm in the z-spectrum, and this was associated with pH values based on the ratio between the creatine (Cr) and the phosphocreatine (PCr). The CEST results demonstrated a significant contrast change at the electrical stimulation site. Moreover, the pH value was observed to decrease from 7.23 to 7.15 within 20 h after electrical stimulation. This pH decrease was verified by 31P magnetic resonance spectroscopy and behavioral tests, which showed a consistent variation over time.

Tetrakis-(N-methyl-4-pyridinium)-porphyrin as a diamagnetic chemical exchange saturation transfer (diaCEST) MRI contrast agent

2021 ◽  
Author(s):  
Subhayan Chakraborty ◽  
Mainak Das ◽  
A. Srinivasan ◽  
Arindam Ghosh
Keyword(s):  
Contrast Agent ◽  
Chemical Exchange ◽  
Mri Contrast Agent ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Mri Contrast ◽  
Wide Range ◽  
Good Contrast

Easily synthesizable tetrakis-(N-methyl-4-pyridinium)-porphyrin as a diaCEST agent that shows nearly pH independent good contrast in a wide range of pH.

Glymphatic System Visualized by Chemical-Exchange-Saturation-Transfer Magnetic Resonance Imaging

2020 ◽  
Vol 11 (13) ◽  
pp. 1978-1984 ◽  
Author(s):  
Yuanfeng Chen ◽  
Zhuozhi Dai ◽  
Ruhang Fan ◽  
David John Mikulis ◽  
Jinming Qiu ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
Saturation Transfer ◽  
Resonance Imaging ◽  
Glymphatic System
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