scholarly journals Fosfomycin enhances phagocyte-mediated killing of Staphylococcus aureus by extracellular traps and reactive oxygen species

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Fengge Shen ◽  
Xudong Tang ◽  
Wei Cheng ◽  
Yang Wang ◽  
Chao Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Extracellular Traps

Piperine exhibits promising antibiofilm activity against Staphylococcus aureus by accumulating reactive oxygen species (ROS)

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Sharmistha Das ◽  
Payel Paul ◽  
Sudipta Chatterjee ◽  
Poulomi Chakraborty ◽  
Ranojit K. Sarker ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Reactive Oxygen ◽  
Antibiofilm Activity ◽  
Oxygen Species

scholarly journals Author Correction: Reactive oxygen species induce antibiotic tolerance during systemic Staphylococcus aureus infection

2020 ◽  
Vol 5 (3) ◽  
pp. 526-526 ◽  
Author(s):  
Sarah E. Rowe ◽  
Nikki J. Wagner ◽  
Lupeng Li ◽  
Jenna E. Beam ◽  
Alec D. Wilkinson ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Reactive Oxygen ◽  
Antibiotic Tolerance ◽  
Oxygen Species ◽  
Aureus Infection

scholarly journals Regulation of Staphylococcus aureus Infection of Macrophages by CD44, Reactive Oxygen Species, and Acid Sphingomyelinase

2018 ◽  
Vol 28 (10) ◽  
pp. 916-934 ◽  
Author(s):  
Cao Li ◽  
Yuqing Wu ◽  
Andrea Riehle ◽  
Véronique Orian-Rousseau ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Reactive Oxygen ◽  
Acid Sphingomyelinase ◽  
Oxygen Species ◽  
Aureus Infection

scholarly journals Statistical classification of dynamic bacterial growth with sub-inhibitory concentrations of nanoparticles and its implications for disease treatment

2020 ◽  
Author(s):  
A-Andrew D Jones ◽  
David Medina-Cruz ◽  
Na Yoon(Julie) Kim ◽  
Gujie Mi ◽  
Caterina Bartomeu-Garcia ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Growth Parameters ◽  
Reactive Oxygen ◽  
Rapid Screening ◽  
Enthalpies Of Formation ◽  
Bacterial Membrane ◽  
Initial Surface ◽  
Oxygen Species ◽  
Development Of Resistance

Nanoparticles are promising alternatives to antibiotics since nanoparticles are easy to manufacture, non-toxic, and do not promote resistance. Nanoparticles act via physical disruption of the bacterial membrane and/or the generation of high concentrations of reactive-oxygen species locally. Potential for physical disruption of the bacterial membrane may be quantified by free energy methods, such as the extended Derjuan-Landau-Verwey-Overbeek theory, which predicts the initial surface-material interactions. The generation of reactive-oxygen species may be quantified using enthalpies of formation to predict minimum inhibitory concentrations. Neither of these two quantitative structure-activity values describes the dynamic, in situ behavioral changes in the bacteria’s struggle to survive. In this paper, borrowing parameters from logistic, oscillatory, and diauxic growth models, we use principal component analysis and agglomerative hierarchical clustering to classify survival modes across nanoparticle types and concentrations. We compare the growth parameters of 170 experimental interactions between nanoparticles and bacteria. The bacteria studied include Escherichia coli, Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Helicobacter pylori, and were tested across multiple concentrations of liposomal drug delivery systems, amphiphilic peptide, and silver and selenium nanoparticles. Clustering reveals specific pairs of bacteria and nanoparticles where the nanoparticle induced growth dynamics could potentially spread the infection through the development of resistance and tolerance. This rapid screening also shows that bacteria generated nanoparticles do not induce growth modes indicative of the development of resistance. This methodology can be used to rapidly screen for novel therapeutics that do not induce resistance before using more robust intracellular content screening. This methodology can also be used as a quality check on batch manufactured nanoparticles.

scholarly journals Piperine Exhibits Promising Antibiofilm Activity Against Staphylococcus Aureus by Accumulating Reactive Oxygen Species (ROS)

2021 ◽  
Author(s):  
Sharmistha Das ◽  
Payel Paul ◽  
Sudipta Chatterjee ◽  
Poulomi Chakraborty ◽  
Ranojit K. Sarker ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Reactive Oxygen ◽  
Antibiofilm Activity ◽  
Host Immune System ◽  
Oxygen Species ◽  
Bacterial Cells ◽  
Protein Assay ◽  
Area Of Concern

Abstract Biofilm, an aggregated form of microbial existence has been a major area of concern in the healthcare units. These sessile microbes not only protect themselves from the host immune system but also exhibit high resistance against several antimicrobials. One such widely reported Gram-positive pathogen is Staphylococcus aureus. This human commensal is known to cause severe harmful diseases like bacteremia, sepsis, pneumonia, etc. Thus, strategies need to be undertaken to deal with such biofilm challenges. In this respect, we aimed to inhibit microbial biofilm formation of Staphylococcus aureus under the influence of a natural compound, piperine. Our study revealed that the higher concentrations of piperine exhibited considerable antimicrobial activity against Staphylococcus aureus. Hence, lower concentrations of piperine were tested to examine its antibiofilm activity. Several experiments like crystal violet (CV) assay, total biofilm protein assay, and fluorescence microscopy observation established that lower concentrations (8 µg/mL and 16 µg/mL) of piperine showed efficient antibiofilm activity against Staphylococcus aureus. It was also noticed that the lower concentrations of piperine did not compromise the microbial growth of Staphylococcus aureus while exhibiting antibiofilm activity. In this connection, we also noticed that the lower concentrations of piperine showed a considerable reduction in microbial metabolic activity. Furthermore, we observed that the compound was found to accumulate reactive oxygen species in the bacterial cells that could play an important role in the inhibition of biofilm formation. Thus, piperine could be considered as a potential antibiofilm agent against the biofilm formation caused by Staphylococcus aureus.

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