scholarly journals Novel Biogenic Silver Nanoparticle-Induced Reactive Oxygen Species Inhibit the Biofilm Formation and Virulence Activities of Methicillin-Resistant Staphylococcus aureus (MRSA) Strain

2020 ◽  
Vol 8 ◽  
Author(s):  
Reham Samir Hamida ◽  
Mohamed Abdelaal Ali ◽  
Doaa A. Goda ◽  
Mahmoud Ibrahim Khalil ◽  
Mayasar Ibrahim Al-Zaban
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticle ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Methicillin Resistant

scholarly journals Piperine Exhibits Promising Antibiofilm Activity Against Staphylococcus Aureus by Accumulating Reactive Oxygen Species (ROS)

2021 ◽  
Author(s):  
Sharmistha Das ◽  
Payel Paul ◽  
Sudipta Chatterjee ◽  
Poulomi Chakraborty ◽  
Ranojit K. Sarker ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Reactive Oxygen ◽  
Antibiofilm Activity ◽  
Host Immune System ◽  
Oxygen Species ◽  
Bacterial Cells ◽  
Protein Assay ◽  
Area Of Concern

Abstract Biofilm, an aggregated form of microbial existence has been a major area of concern in the healthcare units. These sessile microbes not only protect themselves from the host immune system but also exhibit high resistance against several antimicrobials. One such widely reported Gram-positive pathogen is Staphylococcus aureus. This human commensal is known to cause severe harmful diseases like bacteremia, sepsis, pneumonia, etc. Thus, strategies need to be undertaken to deal with such biofilm challenges. In this respect, we aimed to inhibit microbial biofilm formation of Staphylococcus aureus under the influence of a natural compound, piperine. Our study revealed that the higher concentrations of piperine exhibited considerable antimicrobial activity against Staphylococcus aureus. Hence, lower concentrations of piperine were tested to examine its antibiofilm activity. Several experiments like crystal violet (CV) assay, total biofilm protein assay, and fluorescence microscopy observation established that lower concentrations (8 µg/mL and 16 µg/mL) of piperine showed efficient antibiofilm activity against Staphylococcus aureus. It was also noticed that the lower concentrations of piperine did not compromise the microbial growth of Staphylococcus aureus while exhibiting antibiofilm activity. In this connection, we also noticed that the lower concentrations of piperine showed a considerable reduction in microbial metabolic activity. Furthermore, we observed that the compound was found to accumulate reactive oxygen species in the bacterial cells that could play an important role in the inhibition of biofilm formation. Thus, piperine could be considered as a potential antibiofilm agent against the biofilm formation caused by Staphylococcus aureus.

scholarly journals Annihilation of Methicillin-resistant Staphylococcus aureus via Photobleaching of Staphyloxanthin

2017 ◽  
Author(s):  
Pu-Ting Dong ◽  
Haroon Mohammad ◽  
Xiaoyu Wang ◽  
Jie Hui ◽  
Junjie Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Transient Absorption ◽  
Reactive Oxygen ◽  
Imaging Study ◽  
Infection Model ◽  
Oxygen Species ◽  
Methicillin Resistant ◽  
Antibiotic Development ◽  
Absorption Imaging

Given that the dearth of new antibiotic development loads an existential burden on successful infectious disease therapy1, health organizations are calling for alternative approaches to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. Here, we report a drug-free photonic approach to eliminate MRSA through photobleaching of staphyloxanthin, an indispensable membrane-bound antioxidant of S. aureus2-5. The photobleaching process, uncovered through a transient absorption imaging study and quantitated by absorption spectroscopy and mass spectrometry, decomposes staphyloxanthin and sensitizes MRSA to reactive oxygen species attack. Consequently, staphyloxanthin bleaching by low-level blue light eradicates MRSA synergistically with external or internal reactive oxygen species. The effectiveness of this synergistic therapy is validated in MRSA culture, MRSA-infected macrophage cells, S. aureus biofilms, and a mouse wound infection model. Collectively, these findings highlight broad applications of staphyloxanthin photobleaching for treatment of MRSA infections.

scholarly journals Reactive Oxygen Species-Dependent Innate Immune Mechanisms Control Methicillin-Resistant Staphylococcus aureus Virulence in the Drosophila Larval Model

mBio ◽  
2021 ◽  
Author(s):  
Elodie Ramond ◽  
Anne Jamet ◽  
Xiongqi Ding ◽  
Daniel Euphrasie ◽  
Clémence Bouvier ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Virulence Factors ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Innate Immune ◽  
Oxygen Species ◽  
Methicillin Resistant ◽  
Content Type ◽  
Mrsa Strains

The pathogenicity of methicillin-resistant S. aureus (MRSA) strains relies on their ability to produce a wide variety of tightly regulated virulence factors. Current in vivo models to analyze host-pathogen interactions are limited and difficult to manipulate.

scholarly journals l-Ascorbyl 2,6-dipalmitate inhibits biofilm formation and virulence in methicillin-resistant Staphylococcus aureus and prevents triacylglyceride accumulation in Caenorhabditis elegans

RSC Advances ◽  
2017 ◽  
Vol 7 (38) ◽  
pp. 23392-23406 ◽  
Author(s):  
Sivasamy Sethupathy ◽  
Loganathan Vigneshwari ◽  
Alaguvel Valliammai ◽  
Krishnaswamy Balamurugan ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Staphylococcus Aureus ◽  
Caenorhabditis Elegans ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

In the present study, the antibiofilm, antipathogenic and anticarotenogenic potential ofl-ascorbyl 2,6-dipalmitate (ADP) against methicillin-resistantStaphylococcus aureus(MRSA) has been evaluated.

Piperine exhibits promising antibiofilm activity against Staphylococcus aureus by accumulating reactive oxygen species (ROS)

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Sharmistha Das ◽  
Payel Paul ◽  
Sudipta Chatterjee ◽  
Poulomi Chakraborty ◽  
Ranojit K. Sarker ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Staphylococcus Aureus ◽  
Reactive Oxygen ◽  
Antibiofilm Activity ◽  
Oxygen Species
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