scholarly journals Erratum: Corrigendum: Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Hiromitsu Shirasawa ◽  
Jin Kumagai ◽  
Emiko Sato ◽  
Katsuya Kabashima ◽  
Yukiyo Kumazawa ◽  
...  
Keyword(s):  
Electric Field ◽  
Alternating Current ◽  
Immunofluorescence Staining ◽  
Novel Method ◽  
Mammalian Eggs ◽  
Current Electric Field

scholarly journals Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Hiromitsu Shirasawa ◽  
Jin Kumagai ◽  
Emiko Sato ◽  
Katsuya Kabashima ◽  
Yukiyo Kumazawa ◽  
...  
Keyword(s):  
Electric Field ◽  
Three Dimensional ◽  
Alternating Current ◽  
Immunofluorescence Staining ◽  
Ac Electric Field ◽  
Novel Method ◽  
A New Technique ◽  
Antigen Antibody ◽  
First Time ◽  
Mammalian Eggs

Abstract Recently, a new technique was developed for non-catalytically mixing microdroplets. In this method, an alternating-current (AC) electric field is used to promote the antigen–antibody reaction within the microdroplet. Previously, this technique has only been applied to histological examinations of flat structures, such as surgical specimens. In this study, we applied this technique for the first time to immunofluorescence staining of three-dimensional structures, specifically, mammalian eggs. We diluted an antibody against microtubules from 1:1,000 to 1:16,000 and compared the chromatic degree and extent of fading across dilutions. In addition, we varied the frequency of AC electric-field mixing from 5 Hz to 46 Hz and evaluated the effect on microtubule staining. Microtubules were more strongly stained after AC electric-field mixing for only 5 minutes, even when the concentration of primary antibody was 10 times lower than in conventional methods. AC electric-field mixing also alleviated microtubule fading. At all frequencies tested, AC electric-field mixing resulted in stronger microtubule staining than in controls. There was no clear difference in a microtubule staining between frequencies. These results suggest that the novel method could reduce antibody consumption and shorten immunofluorescence staining time.

scholarly journals Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yoshitaro Saito ◽  
Kazuhiro Imai ◽  
Ryuta Nakamura ◽  
Hiroshi Nanjo ◽  
Kaori Terata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electric Field ◽  
Alternating Current ◽  
Novel Method ◽  
Current Electric Field

scholarly journals Effect of Oxidative Stress on Physicochemical Quality of Taiwanese Seagrape (Caulerpa lentillifera) with the Application of Alternating Current Electric Field (ACEF) during Post-Harvest Storage

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1011
Author(s):  
Andi Syahrullah Sulaimana ◽  
Chao-Kai Chang ◽  
Chih-Yao Hou ◽  
Bara Yudhistira ◽  
Fuangfah Punthi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electric Field ◽  
Alternating Current ◽  
Chlorophyll Degradation ◽  
Physicochemical Quality ◽  
Post Harvest ◽  
Caulerpa Lentillifera ◽  
Different Seasons ◽  
Current Electric Field

This study aims to determine the physicochemical quality of seagrape (Caulerpa lentillifera) as a freshness label for products cultivated in different seasons. The applied post-harvest storage experiments compared between, within and without seawater that led to oxidative stress conditions. Water content, malondialdehyde (MDA) compound, total phenolic content (TPC), and chlorophyll content were observed at 0, 3, 6, and 9 days of storage. The storage without seawater showed sharper quality reductions by reaching 20–40% of water loss, 70–90% of MDA production, 15–25% of TPC reduction, and 40–60% of total chlorophyll degradation. The storage within seawater showed lower quality reductions due to the specific growth rates still reaching 5–10%. This study found that the greater the physicochemical quality, the slower the decomposition rates of the stored seagrape during storage. Therefore, the seagrapes’ obvious discoloration occurred earlier in winter, followed by summer and spring. Kinetics of chlorophyll degradation on seagrape in different seasons meet different order-reactions during storage. Furthermore, alternating current electric field (ACEF) treatment with 125 kV/m of intensity for 60 min can lower the spring seagrapes’ physicochemical quality by reaching 10–30% of inhibition, resulting in the shelf-life extension for up to 12 days of post-harvest storage.

Smectic C to Cubic Phase Transition of 4′-n-Docosyloxy-3′-nitrobiphenyl-4-carboxylic Acid (ANBC-22) and Alternating-Current Electric Field Effect

2009 ◽  
Vol 113 (3) ◽  
pp. 640-646 ◽  
Author(s):  
Shoichi Kutsumizu ◽  
Kouhei Hosoyama ◽  
Makoto Yamada ◽  
Katsufumi Tanaka ◽  
Ryuichi Akiyama ◽  
...  
Keyword(s):  
Phase Transition ◽  
Carboxylic Acid ◽  
Electric Field ◽  
Field Effect ◽  
Alternating Current ◽  
Cubic Phase ◽  
Electric Field Effect ◽  
Smectic C ◽  
Current Electric Field
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