scholarly journals Apoptotic effect of novel Schiff Based CdCl2(C14H21N3O2) complex is mediated via activation of the mitochondrial pathway in colon cancer cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Maryam Hajrezaie ◽  
Mohammadjavad Paydar ◽  
Chung Yeng Looi ◽  
Soheil Zorofchian Moghadamtousi ◽  
Pouya Hassandarvish ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Suppressive Effect ◽  
Great Promise ◽  
Potential Candidate ◽  
Colon Cancer Cells ◽  
Extrinsic Pathway ◽  
Apoptotic Pathway ◽  
Induction Of Apoptosis ◽  
Ht 29

Abstract The development of metal-based agents has had a tremendous role in the present progress in cancer chemotherapy. One well-known example of metal-based agents is Schiff based metal complexes, which hold great promise for cancer therapy. Based on the potential of Schiff based complexes for the induction of apoptosis, this study aimed to examine the cytotoxic and apoptotic activity of a CdCl2(C14H21N3O2) complex on HT-29 cells. The complex exerted a potent suppressive effect on HT-29 cells with an IC50 value of 2.57 ± 0.39 after 72 h of treatment. The collapse of the mitochondrial membrane potential and the elevated release of cytochrome c from the mitochondria to the cytosol indicate the involvement of the intrinsic pathway in the induction of apoptosis. The role of the mitochondria-dependent apoptotic pathway was further proved by the significant activation of the initiator caspase-9 and the executioner caspases-3 and -7. In addition, the activation of caspase-8, which is associated with the suppression of NF-κB translocation to the nucleus, also revealed the involvement of the extrinsic pathway in the induced apoptosis. The results suggest that the CdCl2(C14H21N3O2) complex is able to induce the apoptosis of colon cancer cells and is a potential candidate for future cancer studies.

scholarly journals A Schiff Base-Derived Copper (II) Complex Is a Potent Inducer of Apoptosis in Colon Cancer Cells by Activating the Intrinsic Pathway

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Maryam Hajrezaie ◽  
Mohammadjavad Paydar ◽  
Soheil Zorofchian Moghadamtousi ◽  
Pouya Hassandarvish ◽  
Nura Suleiman Gwaram ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Schiff Base ◽  
Cancer Cells ◽  
Chemotherapeutic Agents ◽  
Antiproliferative Effect ◽  
Great Promise ◽  
Potential Candidate ◽  
Colon Cancer Cells ◽  
Potent Inducer ◽  
Ht 29

Metal-based drugs with extensive clinical applications hold great promise for the development of cancer chemotherapeutic agents. In the last few decades, Schiff bases and their complexes have become well known for their extensive biological potential. In the present study, we examined the antiproliferative effect of a copper (II) complex on HT-29 colon cancer cells. The Cu(BrHAP)2Schiff base compound demonstrated a potent antiproliferative effect in HT-29 cells, with an IC50value of 2.87 μg/ml after 72 h of treatment. HT-29 cells treated with Cu (II) complexes underwent apoptosis death, as exhibited by a progressive elevation in the proportion of the G1cell population. At a concentration of 6.25 μg/ml, the Cu(BrHAP)2compound caused significant elevation in ROS production following perturbation of mitochondrial membrane potential and cytochromecrelease, as assessed by the measurement of fluorescence intensity in stained cells. Furthermore, the activation of caspases 3/7 and 9 was part of the Cu (II) complex-induced apoptosis, which confirmed the involvement of mitochondrial-mediated apoptosis. Meanwhile, there was no significant activation of caspase-8. Taken together, these results imply that the Cu(BrHAP)2compound is a potential candidate for furtherin vivoand clinical colon cancer studies to develop novel chemotherapeutic agents derived from metal-based agents.

scholarly journals Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells

2021 ◽  
Vol 18 (8) ◽  
pp. 4166
Author(s):  
Milena Villarini ◽  
Mattia Acito ◽  
Raffaella di Vito ◽  
Samuele Vannini ◽  
Luca Dominici ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Colon Cancer ◽  
Chlorogenic Acid ◽  
Cancer Cells ◽  
Herbaceous Plant ◽  
Colon Cancer Cells ◽  
Leaf Extracts ◽  
Caffeoylquinic Acids ◽  
Induction Of Apoptosis ◽  
Ht 29

(1) Background: Cynara cardunculus L. subsp. scolymus (L.) Hegi, popularly known as artichoke, is an herbaceous plant belonging to the Asteraceae family. Artichoke leaf extracts (ALEs) have been widely used in traditional medicine because of their hepatoprotective, cholagogic, hypoglycaemic, hypolipemic and antibacterial properties. ALEs are also recognized for their antioxidative and anti-inflammatory activities. In this study, we evaluated the cytotoxic, genotoxic, and apoptotic activities, as well as effect on cell growth of ALEs on human colon cancer HT-29 and RKO cells. HT-29 and RKO cells exhibit a different p53 status: RKO cells express the wild-type protein, whereas HT-29 cells express a p53-R273H contact mutant. (2) Methods: Four different ALEs were obtained by sequential extraction of dried artichoke leaves; ALEs were characterized for their content in chlorogenic acid, cynaropicrin, and caffeoylquinic acids. HT-29 and RKO cells were used for in vitro testing (i.e., cytotoxicity and genotoxicity assessment, cell cycle analysis, apoptosis induction). (3) Results: Two out of the four tested ALEs showed marked effects on cell vitality toward HT-29 and RKO tumour cells. The effect was accompanied by a genotoxic activity exerted at a non-cytotoxic concentrations, by a significant perturbation of cell cycle (i.e., with increase of cells in the sub-G1 phase), and by the induction of apoptosis. (4) Conclusions: ALEs rich in cynaropicrin, caffeoylquinic acids, and chlorogenic acid showed to be capable of affecting HT-29 and RKO colon cancer cells by inducing favourable biological effects: cell cycle perturbation, activation of mitochondrial dependent pathway of apoptosis, and the induction of genotoxic effects probably mediated by the induction of apoptosis. Taken together, these results weigh in favour of a potential cancer chemotherapeutic activity of ALEs.

Identification of Nrf2/STAT3 axis in induction of apoptosis through sub‐G 1 cell cycle arrest mechanism in HT‐29 colon cancer cells

2019 ◽  
Vol 120 (8) ◽  
pp. 14035-14043 ◽  
Author(s):  
Issa Tajmohammadi ◽  
Jamal Mohammadian ◽  
Mehdi Sabzichi ◽  
Shiva Mahmuodi ◽  
Mina Ramezani ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Colon Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Cycle Arrest ◽  
Induction Of Apoptosis ◽  
Ht 29

Induction of apoptosis in HT-29 colon cancer cells by crude saponin from Platycodi Radix

2008 ◽  
Vol 46 (12) ◽  
pp. 3753-3758 ◽  
Author(s):  
Jae-Yong Kim ◽  
Kyoung-Wuk Park ◽  
Kwang-Deog Moon ◽  
Mi-Kyung Lee ◽  
Jina Choi ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Platycodi Radix ◽  
Crude Saponin ◽  
Induction Of Apoptosis ◽  
Ht 29

Effect of Sulfur Enriched Young Radish Kimchi on the Induction of Apoptosis in HT-29 Human Colon Cancer Cells

2006 ◽  
Vol 11 (3) ◽  
pp. 184-190 ◽  
Author(s):  
Soon-Sun Bak ◽  
Chang-Suk Kong ◽  
Sook-Hee Rhee ◽  
Chi-Woong Rho ◽  
Nak-Ku Kim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Induction Of Apoptosis ◽  
Human Colon Cancer Cells ◽  
Ht 29

Induction of Apoptosis in HT-29 Colon Cancer Cells by Phloretin

2007 ◽  
Vol 10 (4) ◽  
pp. 581-586 ◽  
Author(s):  
So Young Park ◽  
Eun Ji Kim ◽  
Hyun-Kyung Shin ◽  
Dae Young Kwon ◽  
Myung Sunny Kim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Induction Of Apoptosis ◽  
Ht 29

scholarly journals Effect of sulfur‐enriched young radish kimchi on the induction of apoptosis in HT‐29 human colon cancer cells

2007 ◽  
Vol 21 (6) ◽  
Author(s):  
Soon‐Sun Bak ◽  
Chang‐Suk Kong ◽  
Sook‐Hee Rhee ◽  
Chi‐Woong Rho ◽  
Nak‐Ku Kim ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Induction Of Apoptosis ◽  
Human Colon Cancer Cells ◽  
Ht 29
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