scholarly journals A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jie Shen ◽  
Changzu Cai ◽  
Zhilong Yu ◽  
Yuhong Pang ◽  
Ying Zhou ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Live Cell ◽  
Anthrax Toxin ◽  
Cell Assay ◽  
Cell Lethality

scholarly journals hFUT1-Based Live-Cell Assay To Profile α1-2-Fucoside-Enhanced Influenza Virus A Infection

2020 ◽  
Vol 15 (4) ◽  
pp. 819-823 ◽  
Author(s):  
Senlian Hong ◽  
Geramie Grande ◽  
Chenhua Yu ◽  
Digantkumar G. Chapla ◽  
Natalie Reigh ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Live Cell ◽  
Influenza Virus A ◽  
Cell Assay

Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D

2007 ◽  
Vol 2 (1) ◽  
pp. 187-190 ◽  
Author(s):  
Ingrid Schmid ◽  
Christel Uittenbogaart ◽  
Beth D Jamieson
Keyword(s):  
Flow Cytometry ◽  
Actinomycin D ◽  
Live Cell ◽  
Hoechst 33342 ◽  
Cell Assay ◽  
Dual Laser

scholarly journals A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling

BMC Biology ◽  
2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Edward R. Ballister ◽  
Jessica Rodgers ◽  
Franck Martial ◽  
Robert J. Lucas
Keyword(s):  
Live Cell ◽  
Cell Assay

scholarly journals The heme biosynthetic enzyme, 5-aminolevulinic acid synthase (ALAS), and GTPases in control of mitochondrial dynamics and ER contact sites, regulate heme mobilization to the nucleus

2019 ◽  
Author(s):  
Osiris Martinez-Guzman ◽  
Mathilda M. Willoughby ◽  
Arushi Saini ◽  
Jonathan V. Dietz ◽  
Iryna Bohovych ◽  
...  
Keyword(s):  
Distribution Systems ◽  
Temporal Dynamics ◽  
Aminolevulinic Acid ◽  
Mitochondrial Dynamics ◽  
Live Cell ◽  
Mitochondrial Matrix ◽  
Biosynthetic Enzyme ◽  
Signaling Molecule ◽  
Contact Sites ◽  
Cell Assay

AbstractHeme is an iron-containing cofactor and signaling molecule that is essential for much of aerobic life. All heme-dependent processes in eukaryotes require that heme is trafficked from its site of synthesis in the mitochondria to hemoproteins located throughout the cell. However, the mechanisms governing the mobilization of heme out of the mitochondria, and the spatio-temporal dynamics of these processes, are poorly understood. Herein, using genetically encoded fluorescent heme sensors, we developed a live cell assay to monitor heme distribution dynamics between the mitochondrial inner-membrane, where heme is synthesized, and the mitochondrial matrix, cytosol, and nucleus. We found that heme distribution occurs simultaneously via parallel pathways. In fact, surprisingly, we find that trafficking to the nucleus is ∼25% faster than to the cytosol or mitochondrial matrix. Moreover, we discovered that the heme biosynthetic enzyme, 5-aminolevulinic acid synthase (ALAS), and GTPases in control of the mitochondrial dynamics machinery, Mgm1 and Dnm1, and ER contact sites, Gem1, regulate the flow of heme between the mitochondria and nucleus. Altogether, our results indicate that the nucleus acquires heme faster than the cytosol or mitochondrial matrix, presumably for mitochondrial-nuclear retrograde signaling, and that GTPases that regulate mitochondrial dynamics and ER contact sites are hard-wired to cellular heme distribution systems.Summary StatementThe factors that govern the trafficking of heme, an essential but potentially cytotoxic cofactor and signaling molecule, are poorly understood. Herein, we developed a live-cell assay to monitor heme distribution kinetics and identified the first enzyme in the heme synthesis pathway and GTPases in control of mitochondrial-ER contact sites and dynamics as being critical modulators of heme trafficking.

Live-cell assay to detect the dynamics of protein interactions

2006 ◽  
Author(s):  
Ignacio Demarco ◽  
Cynthia Booker ◽  
Richard Day
Keyword(s):  
Protein Interactions ◽  
Live Cell ◽  
Cell Assay

scholarly journals Anion transport by ortho-phenylene bis-ureas across cell and vesicle membranes

2018 ◽  
Vol 16 (7) ◽  
pp. 1083-1087 ◽  
Author(s):  
Christopher M. Dias ◽  
Hongyu Li ◽  
Hennie Valkenier ◽  
Louise E. Karagiannidis ◽  
Philip A. Gale ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Anion Transport ◽  
Live Cell ◽  
Cell Assay ◽  
Vesicle Membranes

These simple bis-ureas are found to be powerful anionophores in synthetic vesicles, and also in a live cell assay employing yellow fluorescent protein.

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