Enhanced proliferation and differentiation of retinal progenitor cells through a self-healing injectable hydrogel

2019 ◽  
Vol 7 (6) ◽  
pp. 2335-2347 ◽  
Author(s):  
Fang Jiang ◽  
Zhimin Tang ◽  
Yuanhao Zhang ◽  
Yahan Ju ◽  
Huiqin Gao ◽  
...  
Keyword(s):  
Schiff Base ◽  
Progenitor Cells ◽  
Self Healing ◽  
Retinal Progenitor Cells ◽  
Injectable Hydrogel ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation

Fabrication of self-healing injectable CS-Odex hydrogels via a dynamic Schiff-base linkage for RPC delivery.

scholarly journals Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation

2021 ◽  
Author(s):  
Jian Ma ◽  
Xiaoyun Fang ◽  
Min Chen ◽  
Yao Wang ◽  
Li Zhang
Keyword(s):  
Western Blot ◽  
Progenitor Cells ◽  
Neuronal Differentiation ◽  
Collagen Gel ◽  
Time Lapse ◽  
Retinal Progenitor Cells ◽  
Western Blot Assay ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation ◽  
And Migration

Abstract Background: Therapeutic applications of retinal progenitor cells (RPCs) are hindered by their limited proliferation and differentiation capacity and poor ability to migrate into damaged retinal tissue. Our study aimed to explore the effects of HA-CD44 interactions on the regulation of RPCs migration, proliferation and differentiation, and to investigate the underlying regulation mechanisms.Methods: Mouse RPCs were isolated and amplified. Western blot and flow cytometry analyses were used to investigate the expression of CD44 in RPCs. The effects of HA-CD44 interactions on the RPCs behaviors, including migration, proliferation and differentiation, were investigated by MTT assay, CCK8 assay, vertical collagen gel invasion assay, time-lapse imaging, immunocytochemistry, RT-PCR and western blot assay. Furthermore, the downstream signals of HA-CD44 interactions were investigated.Results: CD44 was expressed in RPCs, and HA-CD44 interaction markedly improved RPCs adhesion and migration. The stimulation of miR-21 expression by HA-CD44 interaction was PKC/Nanog-dependent in RPCs. Treatment of RPCs with PKC- or Nanog-specific ASODN or miR-21 antagomir effectively blocked HA-mediated RPCs adhesion and migration. Moreover, ROK/Gab-1 associated PI3K/AKT signaling activation was required in the HA-CD44 interaction mediated RPCs proliferation and neuronal differentiation.Conclusions: Our findings demonstrated new roles for HA-CD44 interaction in regulating both migration, proliferation and neuronal differentiation of RPCs. HA-CD44 signaling could comprise a novel approach to control RPC fates, which may be instructive for the application of RPCs for future therapeutic application.

scholarly journals miR-762 regulates the proliferation and differentiation of retinal progenitor cells by targeting NPDC1

Cell Cycle ◽  
2020 ◽  
Vol 19 (14) ◽  
pp. 1754-1767
Author(s):  
Huiqin Gao ◽  
Ni Ni ◽  
Dandan Zhang ◽  
Yuyao Wang ◽  
Zhimin Tang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation

Reciprocal Actions of microRNA-9 and TLX in the Proliferation and Differentiation of Retinal Progenitor Cells

2014 ◽  
Vol 23 (22) ◽  
pp. 2771-2781 ◽  
Author(s):  
Yamin Hu ◽  
Min Luo ◽  
Ni Ni ◽  
Yuan Den ◽  
Jing Xia ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Proliferation And Differentiation

Different characteristics of rat retinal progenitor cells from different culture periods

2003 ◽  
Vol 341 (3) ◽  
pp. 213-216 ◽  
Author(s):  
Tadamichi Akagi ◽  
Masatoshi Haruta ◽  
Joe Akita ◽  
Akihiro Nishida ◽  
Yoshihito Honda ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Retinal Progenitor Cells ◽  
Retinal Progenitor ◽  
Different Characteristics
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