scholarly journals Ratiometric electrochemical proximity assay for sensitive one-step protein detection

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Kewei Ren ◽  
Jie Wu ◽  
Feng Yan ◽  
Huangxian Ju
Keyword(s):  
Protein Detection ◽  
One Step

scholarly journals One-step split GFP staining for sensitive protein detection and localization in mammalian cells

BioTechniques ◽  
2010 ◽  
Vol 49 (4) ◽  
pp. 727-736 ◽  
Author(s):  
Lara Kaddoum ◽  
Eddy Magdeleine ◽  
Geoffrey S. Waldo ◽  
Etienne Joly ◽  
Stéphanie Cabantous
Keyword(s):  
Mammalian Cells ◽  
Protein Detection ◽  
One Step ◽  
Detection And Localization

scholarly journals Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3195 ◽  
Author(s):  
Hiroki Ashiba ◽  
Chiaki Oyamada ◽  
Kazuya Hosokawa ◽  
Koji Ueno ◽  
Makoto Fujimaki
Keyword(s):  
Waveguide Mode ◽  
Protein Detection ◽  
Sensor Signal ◽  
C Reactive Protein ◽  
Step Method ◽  
Reactive Protein ◽  
Sensor Signals ◽  
Evanescent Light ◽  
One Step ◽  
Surface Performance

One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies were reacted with a target substance and applied onto a biotinylated sensing plate. The target substance was detected by observing changes in sensor signals caused by binding the immunocomplex to the sensing surface. Performance of the developed one-step method was examined using a C-reactive protein (CRP) as a target substance. A sensor signal corresponding to the concentration of CRP was obtained. The minimal detectable CRP concentration of the developed method was 10 pM. The developed method greatly simplifies quantitative protein detection without reducing sensitivity.

Fabrication of hand-in-hand nanostructure for one-step protein detection

2013 ◽  
Vol 49 (36) ◽  
pp. 3760 ◽  
Author(s):  
Chao Li ◽  
Zhaoyin Wang ◽  
Tao Gao ◽  
Aiping Duan ◽  
Genxi Li
Keyword(s):  
Protein Detection ◽  
One Step

A facile one-step gold nanoparticles enhancement based on sequential patterned lateral flow immunoassay device for C-reactive protein detection

2021 ◽  
Vol 329 ◽  
pp. 129241
Author(s):  
Yosita Panraksa ◽  
Amara Apilux ◽  
Sakda Jampasa ◽  
Songchan Puthong ◽  
Charles S. Henry ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
One Step

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

Nutrient-regulated gene expression in eukaryotes

2006 ◽  
Vol 73 ◽  
pp. 85-96 ◽  
Author(s):  
Richard J. Reece ◽  
Laila Beynon ◽  
Stacey Holden ◽  
Amanda D. Hughes ◽  
Karine Rébora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcriptional Control ◽  
Direct Interaction ◽  
Signalling Pathways ◽  
A Cell ◽  
One Step ◽  
Higher Eukaryotes ◽  
Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

Sign in / Sign up

Export Citation Format

Share Document