scholarly journals Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3195 ◽  
Author(s):  
Hiroki Ashiba ◽  
Chiaki Oyamada ◽  
Kazuya Hosokawa ◽  
Koji Ueno ◽  
Makoto Fujimaki
Keyword(s):  
Waveguide Mode ◽  
Protein Detection ◽  
Sensor Signal ◽  
C Reactive Protein ◽  
Step Method ◽  
Reactive Protein ◽  
Sensor Signals ◽  
Evanescent Light ◽  
One Step ◽  
Surface Performance

One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies were reacted with a target substance and applied onto a biotinylated sensing plate. The target substance was detected by observing changes in sensor signals caused by binding the immunocomplex to the sensing surface. Performance of the developed one-step method was examined using a C-reactive protein (CRP) as a target substance. A sensor signal corresponding to the concentration of CRP was obtained. The minimal detectable CRP concentration of the developed method was 10 pM. The developed method greatly simplifies quantitative protein detection without reducing sensitivity.

A facile one-step gold nanoparticles enhancement based on sequential patterned lateral flow immunoassay device for C-reactive protein detection

2021 ◽  
Vol 329 ◽  
pp. 129241
Author(s):  
Yosita Panraksa ◽  
Amara Apilux ◽  
Sakda Jampasa ◽  
Songchan Puthong ◽  
Charles S. Henry ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
One Step

Myocardial Infarction Biomarker C‐reactive Protein Detection on Nanocomposite Aptasensor

2020 ◽  
Author(s):  
Jing Li ◽  
Haitao Li ◽  
Jinpeng Xu ◽  
Xingzhou Zhao ◽  
Shujiang Song ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein

Evaluation of 2-Methacryloyloxyethyl Phosphorylcholine Polymeric Nanoparticle for Immunoassay of C-Reactive Protein Detection

2004 ◽  
Vol 76 (9) ◽  
pp. 2649-2655 ◽  
Author(s):  
Jongwon Park ◽  
Shigeru Kurosawa ◽  
Junji Watanabe ◽  
Kazuhiko Ishihara
Keyword(s):  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Polymeric Nanoparticle

scholarly journals Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

2014 ◽  
Vol 05 (03) ◽  
pp. 153-158 ◽  
Author(s):  
Hanen Chammem ◽  
Imen Hafaid ◽  
Olivier Meilhac ◽  
Farid Menaa ◽  
Laurence Mora ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Plasma ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein

One-step homogeneous C-reactive protein assay for saliva

2011 ◽  
Vol 373 (1-2) ◽  
pp. 19-25 ◽  
Author(s):  
Chamindie Punyadeera ◽  
Goce Dimeski ◽  
Karam Kostner ◽  
Peter Beyerlein ◽  
Justin Cooper-White
Keyword(s):  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein ◽  
One Step

scholarly journals Ultrarapid, Ultrasensitive One-Step Kinetic Immunoassay for C-Reactive Protein (CRP) in Whole Blood Samples: Measurement of the Entire CRP Concentration Range with a Single Sample Dilution

2002 ◽  
Vol 48 (2) ◽  
pp. 269-277 ◽  
Author(s):  
Piia Tarkkinen ◽  
Tom Palenius ◽  
Timo Lövgren
Keyword(s):  
Whole Blood ◽  
Solid Phase ◽  
Point Of Care ◽  
High Sensitivity ◽  
Clinical Samples ◽  
Cardiac Complications ◽  
Fluorescence Signal ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
One Step

Abstract Background: Recently, measurement of very low concentrations of C-reactive protein (CRP) has gained popularity as a potential new means for predicting the risk of future cardiac complications. In this study, we demonstrate the feasibility of a kinetic, one-step microparticle assay for quantitative determination of extremely low and high CRP concentrations in the limited timeframe typical for point-of-care testing. Methods: A noncompetitive, kinetic CRP immunoassay was developed that uses individual, porous microparticles as the solid phase. The microparticles were covalently coated with a monoclonal capture antibody, and the monoclonal detection antibody was labeled with europium. The one-step binding reaction was stopped by washing after 2 min of incubation, and the fluorescence signal of individual particles was measured. Results: The analytical detection limit (mean of zero calibrator + 3 SD) was 0.00016 mg/L CRP. Clinical samples were diluted 400-fold before assay to cover the CRP concentration range of 0.064–1200 mg/L. The assay correlated well with the Dade Behring N High Sensitivity CRP assay (for 0–10 mg/L, r = 0.969, Sy|x = 0.68, n = 54; for 0–350 mg/L, r = 0.969, Sy|x = 11.7, n = 100). The within- and between-run CVs based on calculated concentrations were, respectively, 9–16% and 14% at 0.11 mg/L, 4.5–12% and 8.2% at 4.2 mg/L, and 3.5–6.3% and 4.4% at 105 mg/L, with a CV <15% at 0.2 mg/L and above. Conclusions: Use of the kinetic microparticle approach combined with time-resolved fluorometry allows ultrasensitive quantification of CRP in whole blood in 2 min with a linear assay range spanning more than four orders of magnitude.

Disposable Polydimethylsiloxane/Silicon Hybrid Chips for Protein Detection

2004 ◽  
Author(s):  
Shifeng Li ◽  
David Fozdar ◽  
Dongbing Shao ◽  
Shaochen Chen ◽  
Pierre N. Floriano ◽  
...  
Keyword(s):  
Protein Detection ◽  
Ccd Camera ◽  
Multiplex Assay ◽  
Pdms Layer ◽  
C Reactive Protein ◽  
Fluorescent Assay ◽  
Reactive Protein ◽  
Agarose Beads ◽  
Fluid Network ◽  
Pressure Compensation

This paper presents disposable protein analysis chips with single and multiple chambers - constructed from poly (dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting agarose beads. The sample is introduced into the fluid network in the top PDMS layer where it is directed to the bead chamber. After reaction of the analyte with the probe beads, signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single chamber protein chip was found to be 1ng/mL. Additionally, using the back pressure compensation method, the signals from each of the four-chamber chip were found to be within 10% of each other. Moreover, the fabrication of the multiple-chamber chip may increase throughput and multiplex assay capacity.

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