Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients

2006 ◽  
Vol 1 (6) ◽  
pp. 2643-2649 ◽  
Author(s):  
Trevor G Shepherd ◽  
Brigitte L Thériault ◽  
Elizabeth J Campbell ◽  
Mark W Nachtigal
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Cells ◽  
Cancer Cells ◽  
Primary Culture ◽  
Ovarian Cancer Cells ◽  
Ovarian Surface ◽  
Ovarian Surface Epithelial

scholarly journals Erratum: Corrigendum: Primary culture of ovarian surface epithelial cells and ascites-derived ovarian cancer cells from patients

2015 ◽  
Vol 10 (9) ◽  
pp. 1457-1457 ◽  
Author(s):  
Trevor G Shepherd ◽  
Brigitte L Thériault ◽  
Elizabeth J Campbell ◽  
Mark W Nachtigal
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Cells ◽  
Cancer Cells ◽  
Primary Culture ◽  
Ovarian Cancer Cells ◽  
Ovarian Surface ◽  
Ovarian Surface Epithelial

scholarly journals Evidence and potential clinical significance of changes in gene network interactions in ovarian cancer

2015 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Christopher G. Hill ◽  
John F. McDonald
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Cells ◽  
Cancer Cells ◽  
Gene Network ◽  
Pearson Correlation ◽  
Regulatory Control ◽  
Ovarian Cancer Cells ◽  
New Class ◽  
Regulatory Mutations ◽  
Ovarian Surface Epithelial

The molecular basis of cancer is not merely the consequence of structural and/or regulatory mutations in genes, but additionally to disruptions in networks of regulatory interactions existing among these genes and other components of the genome. Disruptions in network relationships may manifest as the loss, gain or reversal of functionally significant interactive gene relationships in cancer cells. In this study, we first employ an unsupervised (Pearson correlation) approach to quantitatively estimate the overall change in network relationships between precursor (control) ovarian surface epithelial cells and ovarian cancer epithelial cells. We find that ovarian cancer cells display a significant overall reduction in correlated gene network interactions relative to normal precursor cells reflective of an overall loss of regulatory control.  We next focus on gene relationships that qualitatively change between normal and cancer samples. We find that biological processes significantly over represented among differentially expressed genes are substantially different from those associated with genes involved in qualitatively disrupted network interactions. Our findings provide novel insights into the processes underlying ovarian cancer and identify a potential new class of genes for targeted therapy.

scholarly journals Loss of GATA6 Leads to Nuclear Deformation and Aneuploidy in Ovarian Cancer

2009 ◽  
Vol 29 (17) ◽  
pp. 4766-4777 ◽  
Author(s):  
Callinice D. Capo-chichi ◽  
Kathy Q. Cai ◽  
Joseph R. Testa ◽  
Andrew K. Godwin ◽  
Xiang-Xi Xu
Keyword(s):  
Ovarian Cancer ◽  
Nuclear Envelope ◽  
Cancer Cells ◽  
Human Cancer ◽  
Ovarian Surface Epithelium ◽  
Surface Epithelium ◽  
Ovarian Cancer Cells ◽  
Ovarian Surface ◽  
Ovarian Surface Epithelial ◽  
Ovarian Tumorigenesis

ABSTRACT A prominent hallmark of most human cancer is aneuploidy, which is a result of the chromosomal instability of cancer cells and is thought to contribute to the initiation and progression of most carcinomas. The developmentally regulated GATA6 transcription factor is commonly lost in ovarian cancer, and the loss of its expression is closely associated with neoplastic transformation of the ovarian surface epithelium. In the present study, we found that reduction of GATA6 expression with small interfering RNA (siRNA) in human ovarian surface epithelial cells resulted in deformation of the nuclear envelope, failure of cytokinesis, and formation of polyploid and aneuploid cells. We further discovered that loss of the nuclear envelope protein emerin may mediate the consequences of GATA6 suppression. The nuclear phenotypes were reproduced by direct suppression of emerin with siRNA. Thus, we conclude that diminished expression of GATA6 leads to a compromised nuclear envelope that is causal for polyploidy and aneuploidy in ovarian tumorigenesis. The loss of emerin may be the basis of nuclear morphological deformation and subsequently the cause of aneuploidy in ovarian cancer cells.

In vitro model of normal, immortalized ovarian surface epithelial and ovarian cancer cells for chemoprevention of ovarian cancer

2005 ◽  
Vol 98 (2) ◽  
pp. 182-192 ◽  
Author(s):  
Molly Brewer ◽  
J. Taylor Wharton ◽  
Jian Wang ◽  
Amanda McWatters ◽  
Nelly Auersperg ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
In Vitro Model ◽  
Ovarian Cancer Cells ◽  
Ovarian Surface ◽  
Ovarian Surface Epithelial ◽  
Vitro Model

DNA interstrand cross-links induced by cis-dichlorodiammine platinum in ovarian cancer cells growing in primary culture

1988 ◽  
Vol 37 (9) ◽  
pp. 1835-1836
Author(s):  
M. Broggini ◽  
G. Balconi ◽  
F. Bertolero ◽  
E. Erba ◽  
F. Landoni ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Primary Culture ◽  
Ovarian Cancer Cells ◽  
Interstrand Cross Links ◽  
Cross Links
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