scholarly journals Fast, three-dimensional super-resolution imaging of live cells

2011 ◽  
Vol 8 (6) ◽  
pp. 499-505 ◽  
Author(s):  
Sara A Jones ◽  
Sang-Hee Shim ◽  
Jiang He ◽  
Xiaowei Zhuang
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Live Cells ◽  
Resolution Imaging

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

scholarly journals Three-dimensional super-resolution imaging for fluorescence emission difference microscopy

AIP Advances ◽  
2015 ◽  
Vol 5 (8) ◽  
pp. 084901 ◽  
Author(s):  
Shangting You ◽  
Cuifang Kuang ◽  
Shuai Li ◽  
Xu Liu ◽  
Zhihua Ding
Keyword(s):  
Three Dimensional ◽  
Fluorescence Emission ◽  
Super Resolution ◽  
Resolution Imaging

Super-resolution imaging using a three-dimensional metamaterials nanolens

2010 ◽  
Vol 96 (2) ◽  
pp. 023114 ◽  
Author(s):  
B. D. F. Casse ◽  
W. T. Lu ◽  
Y. J. Huang ◽  
E. Gultepe ◽  
L. Menon ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

2021 ◽  
Author(s):  
Anna Loeschberger ◽  
Yauheni Novikau ◽  
Ralf Netz ◽  
Marie-Christin Spindler ◽  
Ricardo Benavente ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Living Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spatiotemporal Resolution ◽  
Coated Pits ◽  
Resolution Imaging

Three-dimensional (3D) multicolor super-resolution imaging in the 50-100 nm range in fixed and living cells remains challenging. We extend the resolution of structured illumination microscopy (SIM) by an improved nonlinear iterative reconstruction algorithm that enables 3D multicolor imaging with improved spatiotemporal resolution at low illumination intensities. We demonstrate the performance of dual iterative SIM (diSIM) imaging cellular structures in fixed cells including synaptonemal complexes, clathrin coated pits and the actin cytoskeleton with lateral resolutions of 60-100 nm with standard fluorophores. Furthermore, we visualize dendritic spines in 70 micrometer thick brain slices with an axial resolution < 200 nm. Finally, we image dynamics of the endoplasmatic reticulum and microtubules in living cells with up to 255 frames/s.

scholarly journals 3D tracking of extracellular vesicles by holographic fluorescence imaging

2020 ◽  
Vol 6 (45) ◽  
pp. eabc2508
Author(s):  
Matz Liebel ◽  
Jaime Ortega Arroyo ◽  
Vanesa Sanz Beltrán ◽  
Johann Osmond ◽  
Ala Jo ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Single Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Fluorescent Detection ◽  
Live Cells ◽  
Fluorescent Light ◽  
3D Tracking ◽  
Anisotropic Motion ◽  
Lag Times

Fluorescence microscopy is the method of choice in biology for its molecular specificity and super-resolution capabilities. However, it is limited to a narrow z range around one observation plane. Here, we report an imaging approach that recovers the full electric field of fluorescent light with single-molecule sensitivity. We expand the principle of digital holography to fast fluorescent detection by eliminating the need for phase cycling and enable three-dimensional (3D) tracking of individual nanoparticles with an in-plane resolution of 15 nm and a z-range of 8 mm. As a proof-of-concept biological application, we image the 3D motion of extracellular vesicles (EVs) inside live cells. At short time scales (<4 s), we resolve near-isotropic 3D diffusion and directional transport. For longer lag times, we observe a transition toward anisotropic motion with the EVs being transported over long distances in the axial plane while being confined in the horizontal dimension.

scholarly journals High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Karl Zhanghao ◽  
Wenhui Liu ◽  
Meiqi Li ◽  
Zihan Wu ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Developmental Stages ◽  
Membrane Separation ◽  
Optical Tomography ◽  
Super Resolution ◽  
Live Cells ◽  
Lipid Dynamics ◽  
Membrane Morphology ◽  
Intracellular Organelles ◽  
Resolution Imaging

AbstractLipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles’ biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome.

scholarly journals Instant super-resolution imaging in live cells and embryos via analog image processing

2013 ◽  
Vol 10 (11) ◽  
pp. 1122-1126 ◽  
Author(s):  
Andrew G York ◽  
Panagiotis Chandris ◽  
Damian Dalle Nogare ◽  
Jeffrey Head ◽  
Peter Wawrzusin ◽  
...  
Keyword(s):  
Image Processing ◽  
Super Resolution ◽  
Live Cells ◽  
Resolution Imaging ◽  
Analog Image Processing
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